Purification and characterization of a maltogenic amylase from Fenugreek (Trigonella foenum graecum) seeds using the Box Benkhen Design (BBD)

Identification of a new oat β -amylase by functional proteomics

“…ACCEPTED MANUSCRIPT 4 then taken up for the growth of the embryo. -amylase and limit dextrinase would occur to attack the linear and branched glucans of starch granule and β-amylase would act on the nonreducing terminus of the linear glucans to release successive maltose units which are degraded finally by the action of -glucosidase to give units of glucose.…”

Optimised amylases extraction from oat seeds and its impact on bread properties

“…In this work, a Box-Behnken design [21][22][23] was set up to look for the best experimental conditions of four independent factors affecting the extraction of the amylase activity namely: the ratio seed weight (g)/buffer volume (ml) (X 1 ), germination days (X 2 ), temperature (X 3 ) and pH (X 4 ) [11].…”

“…1C that there is a gradual increase in the enzyme yields upon increasing the days of germination. Thus, it was implied that an increasing of germination days (7)(8)(9)(10)(11)(12)(13)(14) was favourable for the production of amylase from oat. At 20 • C and pH 7, the amylase yield can reach more than 80 U/g when we use a ratio of 0.1 and germination days upper than 7 days.…”

“…These results were in agreement with others, for example, Uno-Okamura et al [15], who reported an increase in amylase activity of oat coleoptile or leaf by almost 3-fold during germination. Or, Khemakhem et al [11] have shown that extraction of amylase from fenugreek was significantly increased over 7 days. The results presented above showed that the ratio and germination days were the more relevant factors for the amylase yield.…”

Physiological responses of fenugreek seedlings and plants treated with cadmium

“…GA is a classical plant hormone that mediates cell elongation, seed germination, flowering, and other physiological responses inducing synthesis of alpha-amylase activity (Richards et al 2001). In order to verify the involvement of Cd 2+ ions in the disruption of amylase activities during seedling development, the total amylolytic activity was measured under standard conditions defined previously by Khemakhem et al (2012). Amylase activity increased linearly up to 10 days post-germination.…”