Purification and Characterization of a Novel β-Cypermethrin-Degrading Aminopeptidase from <i>Pseudomonas aeruginosa</i> GF31

Tang, Aixing; Liu, Hu; Liu, Youyan; Li, Qingyun; Qing, Yi-Ming

doi:10.1021/acs.jafc.7b03288

Cited by 41 publications

(32 citation statements)

References 41 publications

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“…Proteomics study of strain SG4 has confirmed differential protein expressions in the presence of cypermethrin [17]. Other cypermethrin-degrading microbial genera mainly include Pseudomonas, Micrococcus, Acinetobacter, Aspergillus, Trichoderma, and Candida [1,2,[44][45][46]. These microbes use pyrethroids as the sole sources of carbon and nitrogen to fulfill their nutritional requirements [1].…”

Section: Discussionmentioning

confidence: 99%

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

et al. 2020

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Cypermethrin is popularly used as an insecticide in households and agricultural fields, resulting in serious environmental contamination. Rapid and effective techniques that minimize or remove insecticidal residues from the environment are urgently required. However, the currently available cypermethrin-degrading bacterial strains are suboptimal. We aimed to characterize the kinetics and metabolic pathway of highly efficient cypermethrin-degrading Bacillus thuringiensis strain SG4. Strain SG4 effectively degraded cypermethrin under different conditions. The maximum degradation was observed at 32 °C, pH 7.0, and a shaking speed of 110 rpm, and about 80% of the initial dose of cypermethrin (50 mg·L−1) was degraded in minimal salt medium within 15 days. SG4 cells immobilized with sodium alginate provided a higher degradation rate (85.0%) and lower half-life (t1/2) of 5.3 days compared to the 52.9 days of the control. Bioaugmentation of cypermethrin-contaminated soil slurry with strain SG4 significantly enhanced its biodegradation (83.3%). Analysis of the degradation products led to identification of nine metabolites of cypermethrin, which revealed that cypermethrin could be degraded first by cleavage of its ester bond, followed by degradation of the benzene ring, and subsequent metabolism. A new degradation pathway for cypermethrin was proposed based on analysis of the metabolites. We investigated the active role of B. thuringiensis strain SG4 in cypermethrin degradation under various conditions that could be applied in large-scale pollutant treatment.

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Section: Discussionmentioning

confidence: 99%

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

et al. 2020

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“…Pseudomonas bacterial strains are known for their metabolic capability, environmental versatility and ability to degrade different xenobiotic compounds including permethrin ( Maloney et al, 1988 ), 3-phenoxybenzoate ( Topp and Akhtar, 1991 ), β-cyfluthrin ( Saikia et al, 2005 ), cypermethrin ( Jilani and Khan, 2006 ), diazinon ( Cycoń et al, 2009 ), 4-chloro-2-nitrophenol ( Arora and Bae, 2014 ), 4-chloro-3-nitrophenol ( Arora et al, 2014 ), fenpropathrin ( Song et al, 2015 ), and β-cypermethrin ( Zhang et al, 2011 ; Tang et al, 2017 ). Pseudomonas fulva , one of the highly potent degrading microbes of genus Pseudomonas , is a ubiquitous environmental bacterium.…”

Section: Discussionmentioning

confidence: 99%

“…Hydrolysis plays a significant role in the biodegradation of SPs, which could be attributed to the presence of cyclopropane carboxylic acid moieties connected to aromatic alcohols with a central ester (or ether) bond in SPs. Ester bond is generally considered susceptible to degrading microbes ( Stok et al, 2004 ; Heidari et al, 2005 ; Tallur et al, 2008 ; Wang et al, 2009 ; Zhang et al, 2011 ; Chen et al, 2015 ; Tang et al, 2017 ). In this study, D-phenothrin was initially hydrolyzed via cleavage of the ester bond to yield the main intermediate products 3-phenoxybenzaldehyde and 1,2-benzenedicarboxylic butyl dacyl ester ( Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

“…JZ-1 ( Wang et al, 2009 ), Stenotrophomonas sp. ZS-S-01 ( Chen et al, 2011d ), Ochrobactrum anthropic YZ-1 ( Zhai et al, 2012 ), Brevibacterium aureum DG-12 ( Chen et al, 2013 ), Serratia marcescens ( Cycoń et al, 2014 ), Bacillus thuringiensis ZS-19 ( Chen et al, 2015 ), Pseudomonas aeruginosa GF31 ( Tang et al, 2017 ), and Acinetobacter baumannii ZH-14 ( Zhan et al, 2018b ) have already been isolated from contaminated soils. However, microbial degradation of D-phenothrin and its pathway has never been investigated.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a Pyrethroid-Degrading Pseudomonas fulva Strain P31 and Biochemical Degradation Pathway of D-Phenothrin

et al. 2018

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D-phenothrin is one of the most popular pyrethroid insecticides for its broad spectrum and high insecticidal activity. However, continuous use of D-phenothrin has resulted in serious environmental contamination and raised public concern about its impact on human health. Biodegradation of D-phenothrin has never been investigated and its metabolic behaviors remain unknown. Here, a novel bacterial strain P31 was isolated from active sludge, which completely degraded (100%) D-phenothrin at 50 mg⋅L-1 in 72 h. Based on the morphology, 16S rRNA gene and Biolog tests, the strain was identified as Pseudomonas fulva. Biodegradation conditions were optimized as 29.5°C and pH 7.3 by utilizing response surface methodology. Strain P31 depicted high tolerance and strong D-phenothrin degradation ability through hydrolysis pathway. Strain P31 degraded D-phenothrin at inhibition constant (Ki) of 482.1673 mg⋅L-1 and maximum specific degradation constant (qmax) of 0.0455 h-1 whereas critical inhibitor concentration remained as 41.1189 mg⋅L-1. The 3-Phenoxybenzaldehyde and 1,2-benzenedicarboxylic butyl dacyl ester were identified as the major intermediate metabolites of D-phenothrin degradation pathway through high-performance liquid chromatography and gas chromatography-mass spectrometry. Bioaugmentation of D-phenothrin-contaminated soils with strain P31 dramatically enhanced its degradation, and over 75% of D-phenothrin was removed from soils within 10 days. Moreover, the strain illustrated a remarkable capacity to degrade other synthetic pyrethroids, including permethrin, cyhalothrin, β-cypermethrin, deltamethrin, fenpropathrin, and bifenthrin, exhibiting great potential in bioremediation of pyrethroid-contaminated environment.

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“…Thus, far, many bacterial strains have been isolated to degrade SPs, including Ochrobactrum anthropi YZ-1 (Zhai et al, 2012 ), Brevibacterium aureum DG-12 (Chen et al, 2013 ), Catellibacterium sp. CC-5 (Zhao et al, 2013 ), Serratia marcescens (Cycoń et al, 2014 ), Acinetobacter calcoaceticus MCm5 (Akbar et al, 2015 ), Bacillus thuringiensis ZS-19 (Chen et al, 2015 ), and Pseudomonas aeruginosa GF31 (Tang et al, 2017 ). However, little is known about the kinetics and metabolic behaviors of permethrin.…”

Section: Introductionmentioning

confidence: 99%

Kinetics and Novel Degradation Pathway of Permethrin in Acinetobacter baumannii ZH-14

et al. 2018

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Persistent use of permethrin has resulted in its ubiquitous presence as a contaminant in surface streams and soils, yet little is known about the kinetics and metabolic behaviors of this pesticide. In this study, a novel bacterial strain Acinetobacter baumannii ZH-14 utilizing permethrin via partial hydrolysis pathways was isolated from sewage sludge. Response surface methodology based on Box-Behnken design of cultural conditions was used for optimization resulting in 100% degradation of permethrin (50 mg·L−1) within 72 h. Strain ZH-14 degraded permethrin up to a concentration of 800 mg·L−1. Biodegradation kinetics analysis indicated that permethrin degradation by this strain was concentration dependent, with a maximum specific degradation rate, half-saturation constant, and inhibition constant of 0.0454 h−1, 4.7912 mg·L−1, and 367.2165 mg·L−1, respectively. High-performance liquid chromatography and gas chromatography-mass spectrometry identified 3-phenoxybenzenemethanol and 3-phenoxybenzaldehyde as the major intermediate metabolites of the permethrin degradation pathway. Bioaugmentation of permethrin-contaminated soils with strain ZH-14 significantly enhanced degradation, and over 85% of permethrin was degraded within 9 days with the degradation process following the first-order kinetic model. In addition to degradation of permethrin, strain ZH-14 was capable of degrading a large range of synthetic pyrethroids such as deltamethrin, bifenthrin, fenpropathrin, cyhalothrin, and beta-cypermethrin which are also widely used pesticides with environmental contamination problems, suggesting the promising potentials of A. baumannii ZH-14 in bioremediation of pyrethroid-contaminated terrestrial and aquatic environments.

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Purification and Characterization of a Novel β-Cypermethrin-Degrading Aminopeptidase from Pseudomonas aeruginosa GF31

Cited by 41 publications

References 41 publications

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

Characterization of a Pyrethroid-Degrading Pseudomonas fulva Strain P31 and Biochemical Degradation Pathway of D-Phenothrin

Kinetics and Novel Degradation Pathway of Permethrin in Acinetobacter baumannii ZH-14

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