Purification and Characterization of a Novel Lipase from Antarctic Krill

Chen, Xin; Wang, Chunlan; Xu, Jiakun; Wang, Fang; Jiang, Yihui; Chen, Yixuan; Zhao, Xiaolan

doi:10.1007/s11802-020-4174-1

Cited by 6 publications

(4 citation statements)

References 37 publications

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“…was 37000 Dalton using PAGE -SDS . Tako, et al (25) found the molecular weight of the purified lipase from Rhizopus oryzae was 35000 Dalton .These findings were different from the result recorded by (7,24) that the molecular weight of the purified lipase from Antaractic Krill was 71270 Dalton and Aspergillus terreus reached 46300 Dalton. These variation in molecular weight of lipase could be due the source of enzyme and the methods of determination…”

Section: Figure 1 Ammonium Sulfate Salt Precipitation Of Lipasementioning

confidence: 84%

“…Whereas, (17) mentioned that the optimal pH for the Chinook salmon lipase was 8.5 . Optimum temperature for lipase activity Figure (7) shows the effect of temperature on the activity of the lipase. The enzymatic reaction carried out at different temperature ranging between (20 -90 ° C) with a difference of 5 degrees between one treatment and another.…”

Section: Figure 1 Ammonium Sulfate Salt Precipitation Of Lipasementioning

confidence: 99%

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Extraction, Purification and Characterization of Lipase From the Digestive Duct of Common Carp Cyprinus Carpio L.

Ali¹,

Nasser²,

Shaker³

2022

Iraqi J. Agric. Sci.

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This study was aimed to use digestive duct of common carp Cyprinus Carpio L. as economic source for fish lipase extraction . The enzyme was extracted using potassium phosphate buffer (pH 7 , 0.2 M ). The crude extract then precipitated at the saturation range between 40 – 80 % and dialyzed . Further purified carried out through ion exchange chromatography using DEAE-Cellulose column (2.5 × 30 cm) and gel filtration chromatography by Sephadex G-100 column (1.5 x 60 cm). The purified lipase had a molecular weight of 37.150KDa ,and optimum pH and temperature for enzyme activity about 45◦C and 6.5 using olive oil as substrate .The specific activity, number of purification folds and yield of purified Lipase were 146.48U/mg, 9.76 and 40.50% respectively.

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Section: Figure 1 Ammonium Sulfate Salt Precipitation Of Lipasementioning

confidence: 84%

Section: Figure 1 Ammonium Sulfate Salt Precipitation Of Lipasementioning

confidence: 99%

Extraction, Purification and Characterization of Lipase From the Digestive Duct of Common Carp Cyprinus Carpio L.

Ali¹,

Nasser²,

Shaker³

2022

Iraqi J. Agric. Sci.

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“…They have significant economic value as they can be used as food, oil, medicine, and raw materials in food processing [194]. As most soil/water saline zones are concentrated by the seas/oceans, and due to the vast availability of coastal lands (oceans represent 70% of the Earth's surface), experts predict that marine agriculture, which involves cultivating crops along the coastline, will become an important sustainable solution for the reactivation of these areas [195], especially after the discovery and development of several salt-tolerant crops. For example, some types of Halophytes, called also saltwater vegetables, with varying degrees of salt tolerance, can be irrigated with seawater or planted on salty and alkaline soils.…”

Section: Plantation Of Halophyte Plantsmentioning

confidence: 99%

Revitalization Potential of Marginal Areas for Sustainable Rural Development in the Puglia Region, Southern Italy: Part I: A Review

Alhajj Ali,

Tallou,

Vivaldi

et al. 2024

Agronomy

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Feeding nine billion people by 2050 will be a challenge due to climate change. There is a significant portion of abandoned and unused marginal lands across the nation and in the Puglia region, in Southern Italy. Innovative techniques and practices in the frame of climate-smart agriculture can help rehabilitate marginal lands into productive and profitable areas. The objective of this study was to systematically review the literature on marginal areas in Puglia, responding to the lack of information in this context and evaluate their revitalization potential. We systematically reviewed the literature on unused/marginal areas and identified related studies dealing with different types of marginal areas and their potential for sustainable rural development. Marginal areas in Puglia represent a range of historical rural landscapes that support biodiversity, the economy, and ecological services. However, the analysis of the current situation in Puglia’s marginal areas indicates a lack of infrastructure and scarce resources, which led to land abandonment and the migration of local residents, resulting in the deterioration of the ecological system. Therefore, establishing a sustainable policy is crucial for preserving the local heritage and economy of the region. However, policymakers should carefully study the challenges and opportunities arising from local contexts before embarking on ambitious place-based innovation strategies. The analysis indicates that both biophysical and socio-economic factors are strategic elements for improving the revitalization potential of marginal areas for sustainable development. This review provides useful information regarding the revitalization potential of marginal areas for food, feed, and non-food production, which is crucial in the implementation of a sustainable development strategy for rural communities in Puglia but can also be applied to similar areas in other countries. However, the success of the sustainable development strategy in Puglia’s marginal areas should consider the vital function of farmers’ self-organization and social capital as key factors in the adoption of agricultural innovations for the revitalization of these areas.

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“…Antarctic krill is the most successful single species on the planet, a diversity of endogenous enzymes exist in Antarctic krill, including protease (Sun et al, 2021;Zhou et al, 2018), lipase (Chen et al, 2020), chitinase (El Knidri et al, 2018) and glucosidase (Turkiewicz et al, 2000), and so on. The endogenous enzymes play potential roles in the procedure of fluoride migration which can only be effectively prevented by low temperature (-30 °C) (Sands et al, 1998 .…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of Antarctic krill chitinase and its role on free fluoride release from Antarctic krill cuticle

Peng

2022

Food Sci. Technol

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To investigate factors that influence free fluoride release in Antarctic krill cuticle, the enzyme properties of a chitinase from Antarctic krill were analyzed to identify its role on free fluoride release from Antarctic krill cuticle. The chitinase was purified by ammonium sulfate precipitation,ion and gel chromatography, and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The molecular mass of the chitinase purified from Antarctic krill was 59.0 kDa and its peptide sequences were similar to chitinase precursor sequences from Penaeus vannamei. The optimal pH of the chitinase was pH 6.5 and temperature 45 °C and exhibited high stability within pH 5.0-6.0 and temperatures below 35 °C. The chitinase activity was increased by Ca 2+ and Mg 2+ , while inhibited by Fe 2+ , Cu 2+ and Co 2+ . The purified chitinase had Km and Vmax values of 5.88 mmol/L and 2.11 μmol/min•mL, respectively. The endogenous chitinase purified from Antarctic krill could degrade chitin fibers and induced the release of free fluoride from Antarctic krill cuticle. Under experimental conditions, the free fluoride release law in Antarctic krill cuticle can be described by equation: 0.0111 0.

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Purification and Characterization of a Novel Lipase from Antarctic Krill

Cited by 6 publications

References 37 publications

Extraction, Purification and Characterization of Lipase From the Digestive Duct of Common Carp Cyprinus Carpio L.

Extraction, Purification and Characterization of Lipase From the Digestive Duct of Common Carp Cyprinus Carpio L.

Revitalization Potential of Marginal Areas for Sustainable Rural Development in the Puglia Region, Southern Italy: Part I: A Review

Purification and characterization of Antarctic krill chitinase and its role on free fluoride release from Antarctic krill cuticle

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