Purification and characterization of a Fusarium oxysporum feruloyl esterase (FoFAE-I) catalysing transesterification of phenolic acid esters

Expression, characterization and structural modelling of a feruloyl esterase from the thermophilic fungus Myceliophthora thermophila

“…39 kDa, which is similar to type B Fae-1 from N. crassa expressed in P. pastoris (35 kDa; Crepin et al 2003b) and T. stipitatus TsFaeB (35 kDa;Garcia-Conesa et al 2004). The pH and temperature optima (pH 7 and 50°C) of the recombinant enzyme are in the range reported for type B esterases, such as FoFaeB from F. oxysporum (Topakas et al 2003c), StFaeB from M. thermophila ATCC 34628 (Topakas et al 2004), Fae-1 from N. crassa (Crepin et al 2003b) and also other types of FAEs such as AN1772.2 from A. nidulans (Shin and Chen 2007) and EstA from Piromyces equi (Fillingham et al 1999). The enzyme is stable over a broad range of pH, in accordance with FAEs from the WT strain of M. thermophila ATCC 34628 , FoFaeC from F. oxysporum (Moukouli et al 2008) and AN1772.2 from A. nidulans (Shin and Chen 2007).…”

“…This catalytic behaviour is slightly different from the known type B FAEs from Fae-1 from N. crassa (Crepin et al 2003b), TsFaeB from T. stipitatus (Garcia-Conesa et al 2004), PfFaeB from P. funiculosum (Kroon et al 2000), AoFaeB from A. oryzae (Koseki et al 2009b), AN1772.2 from A. nidulans (Shin and Chen 2007), FoFaeB from F. oxysporum (Topakas et al 2003c) and StFaeB from M. thermophila ATCC 34628 (Topakas et al 2004). In type B esterases, the catalytic preference against the four methyl hydroxycinnamates (MFA, MpCA, MCA and MSA), as described by the catalytic efficiency (k cat /K m ), shows a strong preference on substrates containing one or two hydroxyl substitutions, such as p-coumaric or caffeic acids, following the sequence MpCA>MCA and MFA with no detectable activity against MSA.…”

The Use of a Novel Ferulic Acid Esterase from Lactobacillus acidophilus K1 for the Release of Phenolic Acids from Brewer's Spent Grain

“…There are a significant number of works concerning the purification and characterization of fungal FAEs produced by Aspergillus 9,22,32,40,56,65 , Sporotrichum 58,59 and Fusarium 57,60 species. The number of papers concerning the closer characterization of bacterial FAEs is much lower and some example microorganisms are Streptomyces 20,25,42 and Pseudomonas 24 Clostridium 15,35,41 .…”

Lipase biocatalytic processes in surfactant free microemulsion-like ternary systems and related organogels

“…However, large scale practical application of this enzyme-containing microemulsion may be considerably hindered due to the presence of high concentrations of surfactants as well as by the fact that the solubility of relatively polar phenolic acids in such media is very limited. On the other hand, it is expected that the solubility of such phenolic acids, in surfactant free ternary systems will be much higher than in other organic systems used as reaction media, due to the presence of large amount of the polar alcohol [14,30].…”