Purification and characterization of a feruloyl esterase from Fusarium oxysporum catalyzing esterification of phenolic acids in ternary water–organic solvent mixtures

Topakas, Evangelos; Stamatis, Haralambos; Biely, Peter; Kekos, Dimitris; Macris, Basil J.; Christakopoulos, Paul

doi:10.1016/s0168-1656(02)00363-2

Cited by 116 publications

(72 citation statements)

References 39 publications

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“…However, large scale practical application of this enzyme-containing microemulsion may be considerably hindered due to the presence of high concentrations of surfactants as well as by the fact that the solubility of relatively polar phenolic acids in such media is very limited. On the other hand, it is expected that the solubility of such phenolic acids, in surfactant free ternary systems will be much higher than in other organic systems used as reaction media, due to the presence of large amount of the polar alcohol [14,30].…”

Section: Application Of the Surfactant Free Ternary Systems Containinmentioning

confidence: 99%

Lipase biocatalytic processes in surfactant free microemulsion-like ternary systems and related organogels

Zoumpanioti

Karali

Xenakis

et al. 2006

Enzyme and Microbial Technology

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Section: Application Of the Surfactant Free Ternary Systems Containinmentioning

confidence: 99%

Lipase biocatalytic processes in surfactant free microemulsion-like ternary systems and related organogels

Zoumpanioti

Karali

Xenakis

et al. 2006

Enzyme and Microbial Technology

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“…During the last 16 years, FAEs have gained increased attention as tools for the synthesis of novel hydroxycinnamates with enhanced anti-oxidant activity and tailor-made lipophilicity with potential applications in the food, pharmaceutical, and cosmetics industries [1][2][3][4][5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties

et al. 2018

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Twenty-eight fungal feruloyl esterases (FAEs) were evaluated for their synthetic abilities in a ternary system of n-hexane: t-butanol: 100 mM MOPS-NaOH pH 6.0 forming detergentless microemulsions. Five main derivatives were synthesized, namely prenyl ferulate, prenyl caffeate, butyl ferulate, glyceryl ferulate, and L-arabinose ferulate, offering, in general, higher yields when more hydrophilic alcohol substitutions were used. Acetyl xylan esterase-related FAEs belonging to phylogenetic subfamilies (SF) 5 and 6 showed increased synthetic yields among tested enzymes. In particular, it was shown that FAEs belonging to SF6 generally transesterified aliphatic alcohols more efficiently while SF5 members preferred bulkier L-arabinose. Predicted surface properties and structural characteristics were correlated with the synthetic potential of selected tannase-related, acetyl-xylan-related, and lipase-related FAEs (SF1-2, -6, -7 members) based on homology modeling and small molecular docking simulations.

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“…Subsequently, other in vitro observations confirmed the existence of FAE in animal and human intestines (3, 23). These results suggest that FAE plays an important role in releasing free FA from different feruloylated forms in foodstuff in the gut.Although FAEs from some eukaryotic cells (4,7,11,12,14,22,26,39) and from only one prokaryotic cell (13) have been purified and characterized to date, there is no information about FAE from intestinal bacteria. It is reported that FAEs from various sources show different properties with respect to such characteristics as optimal temperature and optimal pH.…”

mentioning

confidence: 99%

Purification and Characterization of a Feruloyl Esterase from the Intestinal BacteriumLactobacillus acidophilus

Wang

Geng

Egashira

et al. 2004

Appl Environ Microbiol

109

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Dietary ferulic acid (FA), a significant antioxidant substance, is currently the subject of extensive research. FA in cereals exists mainly as feruloylated sugar ester. To release FA from food matrices, it is necessary to cleave ester cross-linking by feruloyl esterase (FAE) (hydroxycinnamoyl esterase; EC 3.1.1.73). In the present study, the FAE from a human typical intestinal bacterium, Lactobacillus acidophilus, was isolated, purified, and characterized for the first time. The enzyme was purified in successive steps including hydrophobic interaction chromatography and anion-exchange chromatography. The purified FAE appeared as a single band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular mass of 36 kDa. It has optimum pH and temperature characteristics (5.6 and 37°C, respectively). Ferulic acid (FA) is a common component in plant cell walls and shows strong antioxidant potential by its radical-scavenging ability (16). In recent years, the results of many in vitro and in vivo studies have indicated that FA prevents low-density lipoprotein from oxidation (2, 27, 29, 31), exhibits inhibitory effects on tumor promotion (1, 17), and protects against certain chronic diseases such as coronary heart disease and some cancers (6,20,21,37).In cereals, FA is mainly ester linked with arabinose or galactose residues (which constitute side chains of cell wall polysaccharides) (15,18,19,28,34,38). Wende et al. showed that FA was released from 2-O-␤-D-xylopyranosyl-(5-O-feruloyl)-Larabinose by rat gut microorganisms quite quickly and presumed that feruloyl esterase (FAE) would be produced by microorganisms, with high-level activity which is closely related to the release (40). Subsequently, other in vitro observations confirmed the existence of FAE in animal and human intestines (3, 23). These results suggest that FAE plays an important role in releasing free FA from different feruloylated forms in foodstuff in the gut.Although FAEs from some eukaryotic cells (4,7,11,12,14,22,26,39) and from only one prokaryotic cell (13) have been purified and characterized to date, there is no information about FAE from intestinal bacteria. It is reported that FAEs from various sources show different properties with respect to such characteristics as optimal temperature and optimal pH. Therefore, it is necessary to purify the FAE from human gut bacteria to further study the mechanism of release of FA from complex food matrices in vitro. Nishizawa et al. studied the FAE activities of typical intestinal bacteria and found that among the intestinal bacteria tested, Lactobacillus acidophilus exhibited the highest level of activity with respect to feruloylated arabinose ester (30).Some investigations indicate that the presence of xylanase enhances FAE activity. In most of these studies, however, destarched wheat bran was used as a substrate; these experiments could not supply sufficiently detailed information to explain the releasing mechanism of dietary FA and the interaction between xylanase and FAE to ...

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Purification and characterization of a feruloyl esterase from Fusarium oxysporum catalyzing esterification of phenolic acids in ternary water–organic solvent mixtures

Cited by 116 publications

References 39 publications

Lipase biocatalytic processes in surfactant free microemulsion-like ternary systems and related organogels

Lipase biocatalytic processes in surfactant free microemulsion-like ternary systems and related organogels

The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties

Purification and Characterization of a Feruloyl Esterase from the Intestinal BacteriumLactobacillus acidophilus

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