Purification and characterization of a 1,3-β-d-glucanase from Streptomyces torulosus PCPOK-0324

Park, Jae Kweon; Kim, Jeong-Dong; Park, Yong Il; Kim, Se‐Kwon

doi:10.1016/j.carbpol.2011.09.058

Cited by 27 publications

(23 citation statements)

References 43 publications

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“…Recently, Park et al . reported synergistic, antifungal activity of 1,3‐β‐D‐glucanase with antibiotic extract from S. torulosus PCPOK0324. Multiplicity of β‐1,3‐glucanases with different molecular weights is also reported in other actinomycetes, such as Cellulosimicrobium cellulans which has isoforms of 40.8, 28.6, and 54.5 kDa .…”

Section: Discussionmentioning

confidence: 99%

“…Zymogram analysis with laminarin as substrate revealed only one enzyme possessing the b-1,3-glucanase activity, with an approximate molecular weight of 131.8 kDa in the mycolytic enzymes of S. violaceusniger MTCC 3959. Recently, Park et al [26] reported synergistic, antifungal activity of 1,3-b-D-glucanase with antibiotic extract from S. torulosus PCPOK0324. Multiplicity of b-1,3-glucanases with different molecular weights is also reported in other actinomycetes, such as Cellulosimicrobium cellulans which has isoforms of 40.8, 28.6, and 54.5 kDa [56].…”

Section: Discussionmentioning

confidence: 99%

“…Actinomycetes (actinobacteria), particularly Streptomyces sp. are known to produce mycolytic enzymes like chitinase , protease , laminarinase (β‐1,3‐glucanase) and also combinations of these enzymes . In the present study, the potential of Streptomyces violaceusniger MTCC 3959 to synthesize mycolytic enzymes which includes chitinase, β‐1,3‐glucanase, and protease was examined.…”

Section: Introductionmentioning

confidence: 98%

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Mycolytic enzymes produced by Streptomyces violaceusniger and their role in antagonism towards wood‐rotting fungi

Nagpure

Choudhary

Gupta

2013

J. Basic Microbiol.

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Extracellular mycolytic enzymes produced under submerged fermentation by the fungal antagonist Streptomyces violaceusniger MTCC 3959 were characterized. This streptomycete produced higher amounts of extracellular chitinase and protease during late exponential phase, whereas β-1,3-glucanase production was at peak in mid-stationary phase. Cell-free culture filtrate (CCF) exhibited a broad range of antifungal activity against both white rot and brown rot fungi. The inhibitory activity was completely lost after treatment with proteinase K and heat, indicating that extracellular antifungal metabolites are heat labile and proteinaceous in nature. Optimum pH and temperature for enzyme activity were: 9.0 and 60 °C for chitinase; 6.0 and 60 °C for β-1,3-glucanase; and 9.0 and 70 °C for protease. Mycolytic enzymes were moderately thermostable, and had a wide pH stability range extending from pH 5.0 to 10.0. The zymogram analysis of CCF revealed five chitinase isoenzymes with an apparent molecular weight of 20.8, 33.3, 45.6, 67.4, and 114.8 kDa, one β-1,3-glucanase appeared as a single band of ∼131.8 kDa and four protease isoenzymes with approximate molecular weights of 22.8, 62.52, 74.64, and 120.5 kDa. S. violaceusniger MTCC 3959 produced mycolytic enzymes that can be effectively used for suppression of phytopathogenic basidiomycetes. It has the potential to be an effective biofungicide.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Mycolytic enzymes produced by Streptomyces violaceusniger and their role in antagonism towards wood‐rotting fungi

Nagpure

Choudhary

Gupta

2013

J. Basic Microbiol.

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“…The antifungal activity of S. lydicus A02 is mainly due to the production of natamycin, a tetraene macrolide antibiotic widely used as a food preservative and fungicide, with potential application for plant fungal disease control in agricultural production [7][8][9][10]. Glucanases and chitinases are pathogenesisrelated (PR) proteins; they can degrade fungal cell walls and have been widely used as antifungal agents in plant protection [11,12]. The wild-type S. lydicus A02 strain exhibited no glucanase activity on CMC-Na plates by congo red staining and very weak chitinase activity when measuring the chitin hydrolysis zones [13].…”

Section: Introductionmentioning

confidence: 99%

Heterologous coexpression of Vitreoscilla hemoglobin and Bacillus megaterium glucanase in Streptomyces lydicus A02 enhanced its production of antifungal metabolites

Dong

et al. 2015

Enzyme and Microbial Technology

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“…Natamycin is a tetraene macrolide antibiotic widely used as a food preservative and fungicide in food, medicine, and veterinary products, and it has the potential to control plant fungal diseases in agricultural production (Ture et al, 2011;Currie et al, 1990;Lu et al, 2008;Wu et al, 2014a). Pathogenesis-related (PR) proteins such as chitinases and glucanases can degrade fungal cell walls and play important roles in plant defence against pathogens, and are widely used as antifungal agents in plant protection (Errakhi et al, 2007;Shi et al, 2010;Park et al, 2012). Paenibacillus polymyxa strains produce several antibiotics and hydrolytic enzymes, which play an important role in the biocontrol of plant pathogens (Deng et al, 2011;Ito and Koyama, 1972;Raza et al, 2009;Beatty and Jensen, 2002).…”

Section: Introductionmentioning

confidence: 99%

Expression of Paenibacillus polymyxa β-1,3-1,4-glucanase in Streptomyces lydicus A01 improves its biocontrol effect against Botrytis cinerea

Liu

Luo

et al. 2015

Biological Control

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h i g h l i g h t sStrain A21 was isolated from special eco-environment in Tibet, China. First report on expression of P. polymyxa glucanase in industrial strain. Enhanced antifungal activity is due to synergy of three factors. a b s t r a c tStreptomyces lydicus strain A01, which can produce natamycin and chitinase, has a significant inhibition effect on gray mold disease caused by Botrytis cinerea. However, it has no detectable glucanase activity. Strain A21 isolated from the snow covered high altitude area in Tibet, China, also has a high antagonistic activity against B. cinerea. It displayed an obvious halo on lichen polysaccharides plates by congo red staining, indicating a strong glucanase activity. A21 was identified as Paenibacillus polymyxa using 16S rDNA gene analysis and biochemical and physiological analysis. To obtain the synergistic antifungal effects of natamycin, chitinase, and glucanases on B. cinerea, this study transformed the b-1,3-1,4-glucanase gene from P. polymyxa A21 to S. lydicus A01. The engineered S. lydicus AG01 showed substantially high glucanase activity, and had similar natamycin production and chitinase activity as the wild-type strain A01. Compared to the wild-type strain A01, the antifungal effects of S. lydicus AG01 on B. cinerea, including inhibition of spore germination and mycelial growth, were highly improved. The improved biocontrol effect of S. lydicus AG01 is likely attributed to the heterologous expression of glucanase from P. polymyxa, which acted synergistically with natamycin and chitinase to increase the antifungal activity of the strain.

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Purification and characterization of a 1,3-β-d-glucanase from Streptomyces torulosus PCPOK-0324

Cited by 27 publications

References 43 publications

Mycolytic enzymes produced by Streptomyces violaceusniger and their role in antagonism towards wood‐rotting fungi

Mycolytic enzymes produced by Streptomyces violaceusniger and their role in antagonism towards wood‐rotting fungi

Heterologous coexpression of Vitreoscilla hemoglobin and Bacillus megaterium glucanase in Streptomyces lydicus A02 enhanced its production of antifungal metabolites

Expression of Paenibacillus polymyxa β-1,3-1,4-glucanase in Streptomyces lydicus A01 improves its biocontrol effect against Botrytis cinerea

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