Purification and characterization of a novel RNase with antiproliferative activity from the mushroom Lactarius flavidulus

Wu, Ying‐Ying; Wang, Hexiang; Ng, T.B.

doi:10.1038/ja.2011.112

Cited by 13 publications

(4 citation statements)

References 35 publications

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“…Zhao et al (16) noted that Schizophyllum commune RNase had no effect on the proliferation of leukemia and lymphoma cells. Only a few mushroom RNases have been shown to inhibit the growth of leukemia cells (36,40,59,60). We found that the anti-proliferative activities of Hohenbuehelia serotina RNase toward L1210 cells are not as remarkable as those of Pleurotus sajor-caju RNase (40), but more effective than those of Hypsizigus marmoreus RNase (45).…”

Section: Inhibition Rates (%)mentioning

confidence: 85%

A novel ribonuclease with antiproliferative activity toward leukemia and lymphoma cells and HIV-1 reverse transcriptase inhibitory activity from the mushroom, Hohenbuehelia serotina

Zhang

Zhao

Wang

et al. 2013

International Journal of Molecular Medicine

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In this study, a 27-kDa ribonuclease (RNase) was purified from the dried fruiting bodies of the mushroom, Hohenbuehelia serotina. The isolation protocol involved anion exchange chromatography, affinity chromatography, cation exchange chromatography and gel filtration in succession. The RNase was unadsorbed on DEAE-cellulose, but was adsorbed on Affi-gel blue gel and CM-cellulose. The N-terminal amino acid sequence was TVGGSLAEKGN which showed homology to other fungal RNases to a certain degree. The RNase exhibited maximal RNase activity at pH 5 and 80˚C. It demonstrated the highest ribonucleolytic activity toward poly(C), a relatively high activity toward poly(U), and a considerably weaker activity toward poly(A) and (G). The RNase inhibited human immunodeficiency virus type 1 (HIV-1) reverse transcriptase with an IC50 of 50 µM and reduced [3H-methyl]-thymidine uptake by L1210 leukemia cells and MBL2 lymphoma cells with an IC50 of 25 µM and 40 µM, respectively.

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Section: Inhibition Rates (%)mentioning

confidence: 85%

A novel ribonuclease with antiproliferative activity toward leukemia and lymphoma cells and HIV-1 reverse transcriptase inhibitory activity from the mushroom, Hohenbuehelia serotina

Zhang

Zhao

Wang

et al. 2013

International Journal of Molecular Medicine

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“…The purified protein was stored in a buffer containing 10 mM Tris-HCl pH7.5, 50 mM NaCl, 4 mM DTT, and 40% glycerol. The ribonucleolytic activity of the purified EDN was assessed using the Kunitz assay that measures the release of nucleotides into the acid soluble fraction [58].…”

Section: Methodsmentioning

confidence: 99%

RNase 2/EDN cleaves the anticodon loops of tRNAs to produce immunostimulatory tRNA halves in asthma

Shigematsu

Pawar

Kawamura

et al. 2019

Preprint

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In the pathogenesis of asthma, inflammatory mediators cause structural and functional changes in resident airway cells including airway smooth muscle (ASM) cells. Here, we report that intranasal treatment of mice with house dust mite (HDM) highly upregulated the expression of tRNA half molecules in asthmatic lungs. The 5′-tRNA haves contain a 2′,3′-cyclic phosphate (cP) and thus belong to cP-containing RNAs (cP-RNAs). Capturing the whole cP-RNA transcriptome using cP-RNA-seq revealed the global upregulation of not only tRNA halves but also the cP-RNAs derived from mRNAs and rRNAs. Our investigation of the biological significance of the major upregulated 5′-tRNA half species in human primary ASM cells revealed that the 5′-tRNA halves repress autophosphorylation at Tyr416 of Src protein kinase, a key regulator of the cellular focal adhesion pathway, leading to reduction of ASM cellular focal adhesion. These results assign a novel role as a protein modification modulator to tRNA half molecules and unveil a tRNA-engaged pathway in the molecular pathogenesis of asthma.

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“…[ 12 ] A 14.6 kDa RNase was purified from the fruit body of Lactarius flavidulus , which inhibited the proliferation of HepG2 cells and L1210 cells. [ 13 ]…”

Section: Introductionmentioning

confidence: 99%

Purification of a novel protein with cytotoxicity against non‐small‐cell lung cancer cells from Boletus bicolor

Zhang

Zhou

Liu

et al. 2021

Archiv der Pharmazie

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A novel protein (D1 component) was purified from Boletus bicolor by ion‐exchange chromatography and gel chromatography on a HiTrap™ Q HP column, a diethylaminoethanol cellulose‐52 column, and a Sephadex G75 column, respectively. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis revealed that the D1 component was a single protein band with a molecular weight of about 40 kDa. The sulforhodamine B assay showed that at concentrations as low as 25–75 μg/ml, protein D1 significantly inhibited the proliferation of human lung adenocarcinoma cell lines (A549 and H1299 cells) and had little effect on human normal kidney cells (HEK293 cells). After labeling protein D1, it was found that D1 could enter the cytoplasm of tumor cells and colocated with lysosomes. Flow cytometry results demonstrated that protein D1 induced apoptosis and G1 phase arrest of the cell cycle in A549 and H1299 cells. The Western blot analysis results showed that the expression of apoptosis and cell cycle‐related proteins of cleaved caspase‐3, cytochrome c, Bax, P16, and P21 was significantly upregulated, whereas the expression of Bcl‐2, CDK4, cyclin D, p‐Rb, and E2F was significantly downregulated after treatment with protein D1. Therefore, D1 exhibits potential to be developed into an antitumor agent.

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Purification and characterization of a novel RNase with antiproliferative activity from the mushroom Lactarius flavidulus

Cited by 13 publications

References 35 publications

A novel ribonuclease with antiproliferative activity toward leukemia and lymphoma cells and HIV-1 reverse transcriptase inhibitory activity from the mushroom, Hohenbuehelia serotina

A novel ribonuclease with antiproliferative activity toward leukemia and lymphoma cells and HIV-1 reverse transcriptase inhibitory activity from the mushroom, Hohenbuehelia serotina

RNase 2/EDN cleaves the anticodon loops of tRNAs to produce immunostimulatory tRNA halves in asthma

Purification of a novel protein with cytotoxicity against non‐small‐cell lung cancer cells from Boletus bicolor

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