Purification and characterization of a Cys-Gly hydrolase from the gastropod mollusk,<i>Patella caerulea</i>

Cappiello, Mario; Spinelli, Matteo; Mormino, Maurizio; Renzone, Giovanni; Scaloni, Andrea; Moschini, Roberta; Balestri, Francesco; Mura, Umberto; Corso, Antonella Del

doi:10.3109/14756366.2016.1158170

Cited by 2 publications

(3 citation statements)

References 42 publications

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“…In this study, Family T3 peptidases (MEROPS database) found in the DG, SG and CG of P. canaliculata (Table S2), fulfill intracellular functions (excepting A0S2T7PH69) as all members have a threonine residue in their catalytic site and are self‐processing proteins with aminopeptidase and aminotransferase activities in their mature forms 30 . Although the physiological role of threonine peptidases of Family T3 eucaryotes is still under study, some reports suggest (a) associations with catalytic subunits of eukaryotic proteasomes where cellular protein turnover occurs, and (b) regulation of prooxidative state by preventing oxidative damage to macromolecules through the regulation of glutathione levels 76,77 …”

Section: Discussionmentioning

confidence: 89%

“…30 Although the physiological role of threonine peptidases of Family T3 eucaryotes is still under study, some reports suggest (a) associations with catalytic subunits of eukaryotic proteasomes where cellular protein turnover occurs, and (b) regulation of prooxidative state by preventing oxidative damage to macromolecules through the regulation of glutathione levels. 76,77 4.2 Diversity and origin of lipases Pomacea canaliculata consumes lipids such as fatty acids. Macrophytes can have ≤4% of fatty acids and triacylglycerols.…”

Section: Threonine Peptidasesmentioning

confidence: 99%

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Proteomic analysis of digestive tract peptidases and lipases from the invasive gastropod Pomacea canaliculata

Escobar‐Correas

Mendoza-Porras

Castro-Vázquez

et al. 2022

Pest Management Science

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Background The invasive gastropod Pomacea canaliculata has received great attention in the last decades as a result of its negative impact on crops agriculture, yet knowledge of their digestive physiology remains incomplete, particularly the enzymatic breakdown of macromolecules such as proteins and lipids. Results Discovery proteomics revealed aspartic peptidases, cysteine peptidases, serine peptidases, metallopeptidases and threonine peptidases, as well as acid and neutral lipases and phospholipases along the digestive tract of P. canaliculata. Peptides specific to peptidases (139) and lipases (14) were quantified by targeted mass spectrometry. Digestion begins in the mouth via diverse salivary peptidases (nine serine peptidases; seven cysteine peptidases, one aspartic peptidase and 22 metallopeptidases) and then continues in the oesophagus (crop) via three luminal metallopeptidases (Family M12) and six serine peptidases (Family S1). Downstream, the digestive gland provides a battery of enzymes composed of aspartic peptidase (one), cysteine peptidases (nine), serine peptidases (12) and metallopeptidases (24), including aminopeptidases, carboxypeptidases and dipeptidases). The coiled gut has M1 metallopeptidases that complete the digestion of small peptides. Lipid extracellular digestion is completed by triglyceride lipases. Conclusion From an integrative physiological and anatomical perspective, P. canaliculata shows an unexpected abundance and diversity of peptidases, which participate mainly in extracellular digestion. Moreover, the previously unknown occurrence of luminal lipases from the digestive gland is reported for the first time. Salivary and digestive glands were the main tissues involved in the synthesis and secretion of these enzymes, but plausibly the few luminally exclusive peptidases are secreted by ventrolateral pouches or epithelial unicellular glands. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 89%

Section: Threonine Peptidasesmentioning

confidence: 99%

Proteomic analysis of digestive tract peptidases and lipases from the invasive gastropod Pomacea canaliculata

Escobar‐Correas

Mendoza-Porras

Castro-Vázquez

et al. 2022

Pest Management Science

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“…In the network (Figure ), glycine and glutamate were the node molecules. Glycine is the smallest of the 20 amino acids commonly found in proteins, but it is not essential to human diet, as it is biosynthesized in the body from the amino acid serine (Cappiello et al, ). In most organisms, the enzyme serine hydroxymethyltransferase catalyzes this transformation via the cafactor pyridoxal phosphate.…”

Section: Discussionmentioning

confidence: 99%

Metabonomic analysis of serum reveals antifatigue effects of Yi Guan Jian on fatigue mice using gas chromatography coupled to mass spectrometry

Shui

Cai

Huang

et al. 2017

Biomedical Chromatography

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Yi Guan Jian (YGJ), one of the most commonly used traditional Chinese medicines, has been reported to possess significant antifatigue effects. However, the mechanisms underlying its antifatigue effects remain largely unresolved. In this study, a metabonomics approach, involving gas chromatography coupled to mass spectrometry and a multivariate statistical technique, was developed to estimate the extent to which YGJ alleviated the exhausting swimming-induced fatigue of mice. High-dose treatment with YGJ significantly extended the swimming time of fatigued mice. Significant alterations of metabolites involving amino acids, organic acids and carbohydrates were observed in the serum of fatigued mice, which were reversed by YGJ treatment while biochemical indexes returned to normal. These metabolic changes suggest that the antifatigue effect of YGJ is associated with the impairement of amino acid, organic acids and carbohydrates. It also appears that YGJ can induce significant metabolic alterations independent of the exhausting swimming-induced metabolic changes. The significantly altered metabolites induced by YGJ intervention include l-2-amino-acetoacetate, taurine, fumaric acid, malic acid, oxoadipic acid and l-aspartate, all of which are associated with antifatigue properties. This suggests that YGJ exerts chemopreventive effects via antifatigue mechanisms.

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Purification and characterization of a Cys-Gly hydrolase from the gastropod mollusk,Patella caerulea

Cited by 2 publications

References 42 publications

Proteomic analysis of digestive tract peptidases and lipases from the invasive gastropod Pomacea canaliculata

Proteomic analysis of digestive tract peptidases and lipases from the invasive gastropod Pomacea canaliculata

Metabonomic analysis of serum reveals antifatigue effects of Yi Guan Jian on fatigue mice using gas chromatography coupled to mass spectrometry

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