“…The enzyme exhibited Michaelis-Menten kinetics in a Triton X-100/ceramide mixed-micellar assay. Moreover, anionic phospholipids, such as PA (phosphatidic acid), PS (phosphatidylserine) and CL (cardiolipin; diphosphatidylglycerol) stimulated the activity, whereas SPH acted as a competitive inhibitor [10]. Rat brain neutral CDase has a preference for ceramide Abbreviations used: CDase, ceramidase; CL, cardiolipin (diphosphatidylglycerol); DEPC, diethyl pyrocarbonate; DFP, di-isopropyl fluorophosphate; dhCer, dihydroceramide; DTT, dithiothreitol; FAAH, fatty acid amide hydrolase; IL-1β, interleukin-1β; maCER1, mouse alkaline CDase; 2-ME, 2-mercaptoethanol; mito-CDase, mitochondrial CDase; NBD, 7-nitrobenz-2-oxa-1,3-diazole; NEM, N-ethylmaleimide; PA, phosphatidic acid; PDGF, platelet-derived growth factor; phytoCer, phytoceramide; PS, phosphatidylserine; S1P, sphingosine 1-phosphate; SPH, sphingosine; YDC1p, dihydroCDase; YPC1p, phytoCDase.…”