Purification and Characterization of a Major Secretory Cellobiase, Cba2, from Cellulomonas biazotea

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

“…β-Glucosidase with broad substrate specificity has been identified from F. oxysporum [121], P. thermophile J18 [59], P. italicum [74], Penicillium simplicissimum [55], A. oryzae [122], Thermobifida fusca [123], and environmental DNA [49]. Cellobiase has been identified from Cellulomonas biazotea [124], and A. niger [52]. Aryl-β-D-glucosidase has been reported in N. fischeri NRRL181 [86] and A. oryzae [125].…”

Cloning and characterization of a novel cellobiase gene, cba3, encoding the first known β-glucosidase of glycoside hydrolase family 1 of Cellulomonas biazotea

“…Unexpectedly, the Cba3 enzyme was identified to be the first representative of glycoside hydrolase family 1 (GH1) in C. biazotea, whereas the previously characterized Cba ) (GenBank ID: AAC38196.1) and Cba2 (Lau and Wong, 2001;Okura et al, 2009) (GenBank ID: DM144958.1) enzymes were shown to be members of glycoside hydrolase family 3 (GH3). Furthermore, based on the comparison of Cba3 with Cba, Cba2 and β-glucosidases of other microorganisms, we discuss the analysis of the physical and enzymatic properties of Cba3.…”

“…Over these years, we have cloned and expressed the cex and cenA genes from Cellulomonas fimi, which encode an Exg and an Eng, respectively, in a wide range of host systems (Fu et al, 2006;Lam et al, 1997Lam et al, , 1998Wang et al, 2010bWang et al, , 2011Wong and Lam, 2000;Wong and Sutherland, 1997;Wong et al, 1988). To search for Cel that might work co-operatively with Exg and Eng, we have put our focus on Cellulomonas biazotea due to the reasons that i) it secretes high levels of Cel activity to the culture medium (Lau and Wong, 2001;Saratale et al, 2010;, and ii) it is closely related to C. fimi (Wood and McCrae Sheila, 1979). Our attempt to demystify the Cel complex of C. biazotea has resulted in the characterization of a couple of secretory Cel: Cba and Cba2 (Lau and Wong, 2001), which will be employed with other Cel, as well as Exg and Eng of C. fimi for reconstitution studies.…”

“…To search for Cel that might work co-operatively with Exg and Eng, we have put our focus on Cellulomonas biazotea due to the reasons that i) it secretes high levels of Cel activity to the culture medium (Lau and Wong, 2001;Saratale et al, 2010;, and ii) it is closely related to C. fimi (Wood and McCrae Sheila, 1979). Our attempt to demystify the Cel complex of C. biazotea has resulted in the characterization of a couple of secretory Cel: Cba and Cba2 (Lau and Wong, 2001), which will be employed with other Cel, as well as Exg and Eng of C. fimi for reconstitution studies. Concurrently, we have also engineered a number of bacterial systems for efficient extracellular production of heterologous proteins (Wong and Lam, 2000;Wong and Sutherland, 1997).…”

“…Our laboratory has been involved in research and application of microbial cellulases for a long time (Fu et al, 2005(Fu et al, , 2006Gilkes et al, 1984;Lam et al, 1997Lam et al, , 1998Lau and Wong, 2001;Skipper et al, 1985;Wang et al, 2010bWang et al, , 2011Warren et al, 1986;Wong and Lam, 2000;Wong and Sutherland, 1997;Wong et al, 1986Wong et al, , 1988. Over these years, we have cloned and expressed the cex and cenA genes from Cellulomonas fimi, which encode an Exg and an Eng, respectively, in a wide range of host systems (Fu et al, 2006;Lam et al, 1997Lam et al, , 1998Wang et al, 2010bWang et al, , 2011Wong and Lam, 2000;Wong and Sutherland, 1997;Wong et al, 1988).…”