Purification and characterization of a platelet aggregation inhibitor and anticoagulant Cc 5_NTase, CD 73‐like, from Cerastes cerastes venom

Abstract

The present study is the first attempt to report the characterization of a nucleotidase from Cerastes cerastes venom. A 70 kDa 5'-nucleotidase (Cc-5'NTase) was purified to homogeneity. The amino acid sequence of Cc-5'NTase displayed high homology with many nucleotidases. Its activity was optimal at pH 7 with a specific hydrolytic activity toward mono-, di-, and triphosphate adenylated nucleotides. Cc-5'NTase preferentially hydrolyzed ADP and obeyed Michaelis-Menten kinetics. Among the metals and inhibitors tested, Ni and Mg completely potentiated enzyme activity, whereas EGTA, PMSF, iodoacetamide, vanillic acid, vanillyl mandelic acid, and 1,10-phenanthroline partially abolished its activity. Cc-5'NTase was not lethal for mice at 5 mg/kg and exhibited in vivo anticoagulant effect. It also dose-dependently inhibited adenosine diphosphate-induced platelet aggregation by converting adenosine diphosphate to adenosine and prohibited arachidonic acid-induced aggregation but was not effective on fibrinogen-induced aggregation. Cc-5'NTase could be a good tool as pharmacological molecule in thrombosis diagnostic and/or therapy.

Identification of lipophilic components in Citri Reticulatae Pericarpium cultivars by supercritical CO2 fluid extraction with ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry

“…Previous studies have shown that organic acids have anti‐inflammatory effects, inhibit platelet aggregation, show anti‐thrombus and antioxidant activities, and induce the apoptosis of cancer cells [16–18]. Here, 10 organic acids were identified in CPR: nicotinic, orsellinic, vanillic, sinapic, ferulic, veratric, 3,4,5‐trimethoxybenzoic, abscisic, artemisinic, and abietic acids.…”

Isolation and characterization of an anti‐leishmanial disintegrin from Cerastes cerastes venom

“…In this study, we report the involvement of a disintegrin in the leish- C. cerastes venom was shown to display antiparasitic and antibacterial activities. [49,50] This venom contains several compounds, including phospholipases A2, [40,51] thrombin-like enzymes, serine proteases, [52][53][54] metalloproteinases, [55][56][57][58] 5-nucleotidase, and C-type lectins [59,60] interfering with different biological processes.…”

Bioactive Molecules Derived from Snake Venoms with Therapeutic Potential for the Treatment of Thrombo-Cardiovascular Disorders Associated with COVID-19

“…These components remain highly relevant for use as experimental tools to elucidate several physiological Fig. 1 Snake venom composition, biological activities and snake venom derived-compounds as drugs [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] (ACh Esterases acetyl choline esterases, SVMPs snake venom metalloproteinases, SVSPs Snake Venom Serine proteinases, SV-PLA2s Snake venom phospho-lipases A2, SV-LAAOs snake venom L-amino acid oxidases, CRISPs cysteine-rich secretory proteins, NGF nerve growth factor, VEGF vascular endothelial growth factor) mechanisms given their selective modes of action. In addition, snake venom derived-compounds can serve as good drugs for developing new biotherapeutics and diagnostics with relevant biomedical applications for many human diseases.…”