Purification and characterization of a novel cellooligosaccharide oxidase from rice pathogen Sarocladium oryzae

Lee, Meng Hwan; Lai, Wen Lin; Lin, Shuen Fuh; Liu, Yu; Hsu, Yuan Hsun; Tsai, Yao‐Chou

doi:10.1016/j.enzmictec.2005.09.011

Cited by 14 publications

(4 citation statements)

References 33 publications

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“…Table presents a summary of the general properties of LOD obtained from M. flexuosum NUK‐21 and those of COX obtained from M. nivale , PCOX obtained from Paraconniothyrium sp. , cellooligosaccharide oxidase (COOX) obtained from S. oryzae , GOOX obtained from Acremonium strictum T1 and cellobiose quinone dehydrogenase (CBG) obtained from P. chrysosporium . All of these five enzymes are monomeric proteins with a molecular weight in the range 51–61 kDa and the molecular weight of the enzyme in the present study is 48 kDa.…”

Section: Discussionmentioning

confidence: 73%

“…Only a few oxidoreductases possess high specificity toward oligosaccharides. These include galactose oxidase obtained from Polyporus circinatus , which exhibits high reactivity to oligosaccharides containing galactose end groups; cellobiose dehydrogenase (EC 1.1.99.18) and cellobiose quinone oxidoreductase obtained from Phanerochaete chrysosporium and Spotoyrichum pulverolentum , which are specific to cellooligosaccharides; carbohydrate:acceptor oxidoreductase (COX) obtained from Microdochium nivale and Sarocladium oryzae that reacts with oligo and polymeric long‐chain carbohydrates ; and COX obtained from Paraconiothyrium sp., which are efficiently oxidized β‐(1‐4) linked sugars, such as lactose, xylobiose and cellooligosaccharides .…”

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confidence: 99%

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Identification of a novel lactose oxidase in Myrmecridium flexuosum NUK‐21

Lin

Chung

2019

FEBS Open Bio

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Lactobionic acid (O‐β‐galactosyl‐(1‐4)‐gluconic acid) (LBA) is a high‐value lactose derivative, produced via oxidation of the reducing terminal of lactose. LBA can be produced by fermentation using certain microorganisms, although subsequent purification is challenging. Therefore, we have attempted to identify an enzyme for possible use in LBA production. Here, we purified a novel lactose oxidase (LOD) to homogeneity from a wheat bran culture of a soil‐isolated fungal strain, Myrmecridium flexuosum NUK‐21. Maximal activity was observed on the wheat bran solid culture after 3 days of NUK‐21 growth, following release from cells at 0.66 unit·mL−1 culture filtrate. This new sugar oxidase was composed of a single polypeptide chain with a molecular mass of 47.2 kDa and was found to contain 2.0 zinc ions per mole of enzyme but no flavin adenine dinucleotide or heme. This enzyme was stable in the pH range 5.5–9.0, with an optimal reaction pH of 7.5. Its optimal reaction temperature was 40 °C, and it was stable up to 50 °C for 1 h at pH 7.5. LOD oxidized disaccharides with reducing‐end glucosyl residues linked by an α or β‐1,4 glucosidic bond. The relative activity of LOD toward lactose, cellobiose and maltose was 100 : 83 : 4, respectively. To the best of our knowledge, this is the first report on the discovery of an LOD based on coenzyme moiety and enzyme substrate specificity.

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Section: Discussionmentioning

confidence: 73%

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confidence: 99%

Identification of a novel lactose oxidase in Myrmecridium flexuosum NUK‐21

Lin

Chung

2019

FEBS Open Bio

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“…Glucooligosaccharide oxidase from Acremonium strictum T1 [18], glycerol oxidase from Penicillium TS-625 [17], and cellooligosaccharide oxidase from Sarocladium oryzae F-137 [16] were obtained in this screening program. In these assays, oxidase activity is determined by spectrophotometrically following continuous 4-AA reduction.…”

Section: Discussionmentioning

confidence: 99%

Borate–fructose complex: A novel mediator for laccase and its new function for fructose determination

2015

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a b s t r a c tLaccase and borate-fructose complex were investigated by coincidence in a solid-state fermentation of Edenia sp. TS-76 under fructose oxidase screening. Laccase was purified to homogeneity with a 34-fold purification and 32% yield. Fructose had no significant effect on laccase activity, whereas borate reduced laccase activity by 60-90%; conversely, the borate-fructose complex increased laccase activity by nearly fourfold at pH 7.5. The complex caused a shift in the optimal pH for laccase from 5.0 to 7.5 and served as a highly efficient mediator. Borate complexed with fructose provides an alternative, time-saving, and specific method for serum fructose determination.

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“…Degrading Bacteria: Some rumen anaerobic fungi can secrete cellulase with high activity and degrade cellulose, hemicellulose and even lignin in many plant cells walls (Wei et al, 2016;Wood et al, 1986). Sarocladium can secrete cello-oligosaccharide oxidase (Lee et al, 2006).…”

Section: Effects Of Mannan Oligosaccharides On Rumen Fibermentioning

confidence: 99%

Effects of Mannan Oligosaccharides on Rumen Fungal Flora of Dairy Cows

2021

THE JAPS

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To study the effects of mannan oligosaccharides (MOS) on rumen fungal flora of dairy cows, four lactating Chinese Holstein dairy cows were randomly divided into two groups in the two-stage 2 × 2 cross-over design. The experimental group (MT) was fed with a basal ration and MOS were perfused orally 60 g/(d head). The control group (CK) was fed with a basal ration. The rumen fluid was collected through the oral cavity of dairy cows using a negative pressure suction device. 50ml rumen fluid of every cow was collected each time respectively before feeding (0 h) and 2h, 4h and 6h after feeding in a day. The rumen fungi were tested by ITS sequencing technology. The results showed that four phyla were identified in CK group and five in MT group. The dominant phyla in both groups were Ascomycota, Basidiomycota, Neocallimastigomycota, whose abundances were more than 90% of the total rumen fungi. At the level of genus, 74 genera were identified in CK group, while 91 genera in MT group. Several cellulolytic fungi genera showed a decreasing trend or decreased significantly, such as Neocallimastigaceae-NA. The previous experiments that shows the addition of mannan oligosaccharides promoted the proliferation of rumen bacteria, while the growth of rumen fungi was inhibited in this result, which may be due to the antagonism between rumen bacteria and fungi. The growth of cellulose-degrading fungi was inhibited to some extend after the addition of MOS in the diet. Candida and Zopfiella, closely related to mycotic mastitis and immunosuppression respectively, had decreasing trends in rumen with supplemental MOS in the diets of the dairy cows, indicating that MOS might play a positive role in inhibiting harmful fungi. The experimental result shows that mannan oligosaccharides in the diet of dairy cows inhibited the growth of cellulolytic fungi and the proliferation of harmful fungi. In addition, the results showed that there might be the antagonistic effect between rumen fungi and bacteria potentially, which should be focused on for further study.

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Purification and characterization of a novel cellooligosaccharide oxidase from rice pathogen Sarocladium oryzae

Cited by 14 publications

References 33 publications

Identification of a novel lactose oxidase in Myrmecridium flexuosum NUK‐21

Identification of a novel lactose oxidase in Myrmecridium flexuosum NUK‐21

Borate–fructose complex: A novel mediator for laccase and its new function for fructose determination

Effects of Mannan Oligosaccharides on Rumen Fungal Flora of Dairy Cows

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