Purification and characterization of a β‐1,4‐endoxylanase from the ericoid mycorrhizal fungus <i>Hymenoscyphus ericae</i>

Burke, R. M.; Cairney, John

doi:10.1046/j.1469-8137.1997.00634.x

Cited by 26 publications

(12 citation statements)

References 48 publications

(58 reference statements)

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“…Nevertheless, compared with these activities, A. zeae EA0802 culture with corn straw or oat spelt xylan produced smaller xylanase activity of 0.76 UmL −1 , on ninth day and 1.56 UmL −1 after 15 days of growth, respectively (Table 1). Hymenoscyphus ericae, an endophyte fungus, produced two xylanase forms, with a total activity of 5.8 UmL −1 , in liquid medium with oat spelt xylan [48]. Rhizopus oryzae produced 0.39 U mL −1 using 1% xylan as carbon source after 6 days, and when 2% corn cob was used, the fungi produced 2.8 UmL −1 after 5 days [49].…”

Section: Xylanasementioning

confidence: 99%

Cellulases and Hemicellulases from Endophytic Acremonium Species and Its Application on Sugarcane Bagasse Hydrolysis

Almeida

Guimarães

Bischoff

et al. 2011

Appl Biochem Biotechnol

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The aim of this work was to have cellulase activity and hemicellulase activity screenings of endophyte Acremonium species (Acremonium zeae EA0802 and Acremonium sp. EA0810). Both fungi were cultivated in submerged culture (SC) containing L -arabinose, D -xylose, oat spelt xylan, sugarcane bagasse, or corn straw as carbon source. In solid-state fermentation, it was tested as carbon source sugarcane bagasse or corn straw. The highest FPase, endoglucanase, and xylanase activities were produced by Acremonium sp. EA0810 cultivated in SC containing sugarcane bagasse as a carbon source. The highest β-glucosidase activity was produced by Acremonium sp. EA0810 cultivated in SC using D -xylose as carbon source. A. zeae EA0802 has highest α-arabinofuranosidase and α-galactosidase activities in SC using xylan as a carbon source. FPase, endoglucanase, β-glucosidase, and xylanase from Acremonium sp. EA0810 has optimum pH and temperatures of 6.0, 55 °C; 5.0, 70 °C; 4.5, 60 °C; and 6.5, 50 °C, respectively. α-Arabinofuranosidase and α-galactosidase from A. zeae EA0802 has optimum pH and temperatures of 5.0, 60 °C and 4.5, 45 °C, respectively. It was analyzed the application of Acremonium sp. EA0810 to hydrolyze sugarcane bagasse, and it was achieved 63% of conversion into reducing sugar and 42% of conversion into glucose.

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Section: Xylanasementioning

confidence: 99%

Cellulases and Hemicellulases from Endophytic Acremonium Species and Its Application on Sugarcane Bagasse Hydrolysis

Almeida

Guimarães

Bischoff

et al. 2011

Appl Biochem Biotechnol

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“…Cellobiose was also used as a carbon source in each isolate tested. Cellobiose can be transported directly or following cleavage into glucose molecules via an extracellular cellobiase before absorption (Griffin, 1994;Burke and Cairney, 1997).…”

Section: Carbonmentioning

confidence: 99%

Variation in nutrient-acquisition patterns by mycorrhizal fungi of rare and common orchids explains diversification in a global biodiversity hotspot

et al. 2013

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These results suggest that OMF can differentiate between niches (micro-niche specialization) in a constrained, highly resource-limited environment such as the SWAFR. Phosphorus is the most limited macronutrient in SWAFR soils and the ability to access phytate by OMF indicates a characterizing functional capacity of OMF from the SWAFR. Furthermore, compared with OMF isolated from the rare D. elastica, OMF associating with the common P. recurva produced far greater biomass over a wider variety of nutritional sources. This suggests a broader tolerance for habitat variation providing more opportunities for the common orchid for recruitment and establishment at a site.

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“…However, there are few studies reported on utilization of plants as sources of endophytic xylanase producer (Burke andChairney 1997, Suto et al 2002) and no work presented on xylanolytic endophyte isolation from maize. This is the first report presenting the screening of maize for xylanolytic bacteria isolation.…”

Section: Xylanolytic Microorganism Isolationmentioning

confidence: 99%

“…Due to hemicellulosic (xylan) component of plant cell wall, existence of endophytic xylanolytic microorganisms is expected. Few endophytic xylanase producers have been reported up to date (Burke et al 1997, Suto et al 2002, Ersay ı n et al 2007.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Endopyhtic and Xylanolytic Bacillus pumilus Strains from Zea mays

YAŞINOK¹,

Ayşegül;ŞAHİN²,

İffet;HABERAL³

et al. 2008

Tarım Bilimleri Dergisi

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In this study, two endophytic xylanolytic bacteria, named M1 and M2, were isolated from surface sterilized Zea mays stern and leaf, respectively. Isolates were identified as Bacillus pumilus. Microorganisims showed different morphology on agar plates. Xylanase production level and profile varied between isolates, as well. Maximum xylanase production level of 188.0 ± 20.0 and 5.6 ± 1.1 U/ml were achieved by Bacillus pumilus M1 and Bacillus pumilus M2 in a liquid medium containing 3% corn cobs as a sole carbon source and inducer, respectively. Isolates produced very low level of cellulase in crude enzyme extract. B. pumilus M1 xylanase was partially characterized. Enzyme had a maximum activity at pH 8.0 and 65°C, seemed proper for pulp and paper industry, and required further characterization. In conclusion, this study indicated that inside part of Zea mays is a novel and good source for isolating xylanase producers. After detailed characterization, such enzymes could be used in various applications in paper and pulp, textile, food and feed industries.Key Words: Bacillus pumilus, endopyhte, plant, xylan, xylanase producers. Zea mays'dan Endofitik ve Ksilanolitik Bacillus pumilus Suş ları n ı n İzolasyonuÖz: Bu çal ışmada, yüzey sterilizasyonu yap ı lm ış Zea mays gövde ve yapraklar ı ndan, s ı ras ı ile M1 ve M2 olarak isimlendirilen iki tane endofitik ksilanolitik bakteri izole edilmi ştir. Izolatlar Bacillus pumilus olarak tan ı mlanm ıştı r. Mikroorganizmalar agar üzerinde farkl ı morfoloji sergilemi ştir. Izolatlar aras ı nda, ksilanaz üretim seviyesi ve profilinde de farkl ı l ı klar bulunmaktad ı r. İçinde %3 m ı s ı r koçan ı n ı tek karbon kaynağı olarak bulunduran s ı v ı besi yerinde, Bacillus pumilus Mi ve Bacillus pumilus M2 s ı ras ı ile maksimum ksilanaz aktivitesi olan 188.0 ± 20.0 ve 5.6 t 1.1 U/mi değ erlerine ula şabilmi ştir. İzolatlar, kaba fermentasyon özütünde çok dü ş ük miktarda selülaz üretmi ştir. B. pumilus M1 ksilanaz ı k ı smi karakterize edilmi ştir. Enzim pH 8.0 ve 65°C'de maksimum aktivite götermi ş , ka ğı t beyazlatma endüstrisine uygun görülmü ş ve ileri derecede karakterizasyona ihtiyaç duymu ştur. Sonuç olarak, bu çal ış ma Zea mays' ı n iç k ı sm ı n ı n, ksilanaz üreticilerinin izolasyonu için yeni ve iyi bir kaynak oldu ğ unu göstermi ştir. Detayl ı tan ı mlama ard ı ndan bu enzimler, ka ğı t, tekstil, g ı da ve yem endüstrisinde çe ş itli uygulamalarda değerlendirilebilmesi mümkündür.

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Purification and characterization of a β‐1,4‐endoxylanase from the ericoid mycorrhizal fungus Hymenoscyphus ericae

Cited by 26 publications

References 48 publications

Cellulases and Hemicellulases from Endophytic Acremonium Species and Its Application on Sugarcane Bagasse Hydrolysis

Cellulases and Hemicellulases from Endophytic Acremonium Species and Its Application on Sugarcane Bagasse Hydrolysis

Variation in nutrient-acquisition patterns by mycorrhizal fungi of rare and common orchids explains diversification in a global biodiversity hotspot

Isolation of Endopyhtic and Xylanolytic Bacillus pumilus Strains from Zea mays

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