Purification and characterization of a novel mixed-linkage α,β-d-glucan from Arca subcrenata and its immunoregulatory activity

Shi, Hui; Bi, Sixue; Li, Hang; Li, Jianhuan; Li, Chunlei; Yu, Rongmin; Song, Liyan; Zhu, Jianhua

doi:10.1016/j.ijbiomac.2021.03.196

Cited by 13 publications

(11 citation statements)

References 52 publications

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“…The 1 H and 13 C NMR spectra of PSP-1 are shown in Figures 3A,B . The entire assignment shifts of 1 H and 13 C NMR for PSP-1 were identified with reference to the previous literatures ( 35 , 37 – 39 ) and illustrated in Table 2 . The anomeric proton signals (5.33, 4.89, and 4.46 ppm) and the anomeric carbon signals (99.6, 97.9, and 102.9 ppm) corresponded to H-1 and C-1 of → 4)-α-D-Glc p -(1 →, → 4,6)-α-D-Glc p -(1 →, and β-D-Glc p -(1 →.…”

Section: Resultsmentioning

confidence: 99%

Anti-cancer Potential of Polysaccharide Extracted From Polygonatum sibiricum on HepG2 Cells via Cell Cycle Arrest and Apoptosis

Liu²,

Zhang³

et al. 2022

Front. Nutr.

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Polygonatum sibiricum is one of the most widely used traditional Chinese medicine in China. Polygonatum sibiricum polysaccharide (PSP) is the main functional component of Polygonatum sibiricum. In this study, a water-soluble polysaccharide (PSP-1) was first isolated from Polygonatum sibiricum with a molecular weight of 38.65 kDa. Structural analysis was performed via methylation and FT-IR spectroscopy analyses, which in combination with NMR spectroscopy, revealed that PSP-1 has a → 4-α-D-Glcp-1 → backbone with the substitution at O-6 with the β-D-Glcp-1 → residues. Furthermore, PSP-1 exhibited potent and concentration-dependent anticancer effects, inducing HepG2 cell apoptosis and arresting the cell cycle at the G1 phase. Moreover, PSP-1 also decreased the mitochondrial membrane potential, damaged the nucleus of HepG2 cells, and increased the activity of caspase-9 and−3 in the intrinsic apoptotic pathways to induce HepG2 cell apoptosis. To conclude, PSP-1 might be a good candidate for the treatment of liver cancer, and this work provides important information for understanding the relationship between structure and antitumor activity of PSP-1, which is relevant for the treatment of hepatocellular carcinoma in clinic.

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Section: Resultsmentioning

confidence: 99%

Anti-cancer Potential of Polysaccharide Extracted From Polygonatum sibiricum on HepG2 Cells via Cell Cycle Arrest and Apoptosis

Liu²,

Zhang³

et al. 2022

Front. Nutr.

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“…The proposed α-(1 → 6)-glucan and α-(1 → 4,6)-glucan backbone of ASPG-1 isolated from A. subcrenata differs from the previously reported α,β- d -glucan. 24…”

Section: Discussionmentioning

confidence: 99%

“…ASPG-1 was completely methylated by according our previous studies. 24 ASPG-1 (10 mg) and NaOH powder (600 mg) were weighed and dissolved in anhydrous DMSO. Next, the sample was methylated for 1 h in the dark by the addition of methyl iodide, and the reaction was terminated with distilled water.…”

Section: Methodsmentioning

confidence: 99%

Characterization of a novel polysaccharide from Arca subcrenata and its immunoregulatory activities in vitro and in vivo

Shi

Liu

et al. 2023

Food Funct.

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“…The anomeric carbon signals appeared at 104.99, 104.42, and 104.09 ppm, and were ascribed to C-1 of β-D-galactopyranose units [18]. The signal at 99.43 ppm could be the C-1 of α-D-glucopyranose residue [19]. The 1 H-1 H COSY spectrum of dsOCSH4 demonstrated the proton correlations of the sugar residues.…”

Section: Structural Characteristics Of the Sulfated Polysaccharidementioning

confidence: 94%

Chemical Structure and Anticoagulant Property of a Novel Sulfated Polysaccharide from the Green Alga Cladophora oligoclada

Yang

Shao

et al. 2021

Marine Drugs

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Marine macroalgae are efficient producers of sulfated polysaccharides. The algal sulfated polysaccharides possess diverse bioactivities and peculiar chemical structures, and represent a great potential source to be explored. In the present study, a heparinoid-active sulfated polysaccharide was isolated from the green alga Cladophora oligoclada. Results of chemical and spectroscopic analyses indicated that the sulfated polysaccharide was composed of →6)-β-d-Galp-(1→, β-d-Galp-(1→, →6)-α-d-Glcp-(1→ and →3)-β-d-Galp-(1→ units with sulfate esters at C-2/C-4 of →6)-β-d-Galp-(1→, C-6 of →3)-β-d-Galp-(1→ and C-3 of →6)-α-d-Glcp-(1→ units. The branches consisting of β-d-Galp-(1→ and →6)-β-d-Galp-(1→ units were located in C-3 of →6)-β-d-Galp-(1→ units. The sulfated polysaccharide exhibited potent anticoagulant activity in vitro and in vivo as evaluated by activated partial thromboplastin time (APTT), thrombin time, and the fibrinogen level. For the APTT, the signal for clotting time was more than 200 s at 100 μg/mL in vitro and at 15 mg/kg in vivo. The obvious thrombolytic activity of the sulfated polysaccharide in vitro was also found. The mechanism analysis of anticoagulant action demonstrated that the sulfated polysaccharide significantly inhibited the activities of all intrinsic coagulation factors, which were less than 1.0% at 50 μg/mL, but selectively inhibited common coagulation factors. Furthermore, the sulfated polysaccharide strongly stimulated the inhibition of thrombin by potentiating antithrombin-III (AT-III) or heparin cofactor-II, and it also largely promoted the inhibition of factor Xa mediated by AT-III. These results revealed that the sulfated polysaccharide from C. oligoclada had potential to become an anticoagulant agent for prevention and therapy of thrombotic diseases.

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Purification and characterization of a novel mixed-linkage α,β-d-glucan from Arca subcrenata and its immunoregulatory activity

Cited by 13 publications

References 52 publications

Anti-cancer Potential of Polysaccharide Extracted From Polygonatum sibiricum on HepG2 Cells via Cell Cycle Arrest and Apoptosis

Anti-cancer Potential of Polysaccharide Extracted From Polygonatum sibiricum on HepG2 Cells via Cell Cycle Arrest and Apoptosis

Characterization of a novel polysaccharide from Arca subcrenata and its immunoregulatory activities in vitro and in vivo

Chemical Structure and Anticoagulant Property of a Novel Sulfated Polysaccharide from the Green Alga Cladophora oligoclada

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