Purification and characterization of 5-oxo- l -prolinase from Paecilomyces varioti F-1, an ATP-dependent hydrolase active with l -2-oxothiazolidine-4-carboxylic acid

Mochizuki, Kazuya

doi:10.1007/s002030050758

Cited by 6 publications

(4 citation statements)

References 9 publications

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“…Protein extracts of the oxp1-1 mutant incubated with pCys showed a significantly lower rate of Cys production than the WT as observed for the Glu production from 5OP, supporting that At OXP1 can hydrolyze pCys, as reported for OPases in other organisms ( Fig. 4 F and G ) ( 43 , 45 ). Notably, extracts of the WT seedlings cultured at S15 condition exhibited increased OPase activity against both pCys and 5OP, which were 5.6- and 2.9-fold higher than those cultured at S1500, respectively ( Fig.…”

Section: Resultssupporting

confidence: 83%

Retrograde sulfur flow from glucosinolates to cysteine in Arabidopsis thaliana

Sugiyama

Kuwahara

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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Specialized (secondary) metabolic pathways in plants have long been considered one-way routes of leading primary metabolite precursors to bioactive end products. Conversely, endogenous degradation of such “end” products in plant tissues has been observed following environmental stimuli, including nutrition stress. Therefore, it is of general interest whether specialized metabolites can be reintegrated into primary metabolism to recover the invested resources, especially in the case of nitrogen- or sulfur-rich compounds. Here, we demonstrate that endogenous glucosinolates (GLs), a class of sulfur-rich plant metabolites, are exploited as a sulfur source by the reallocation of sulfur atoms to primary metabolites such as cysteine in Arabidopsis thaliana. Tracer experiments using 34S- or deuterium-labeled GLs depicted the catabolic processing of GL breakdown products in which sulfur is mobilized from the thioglucoside group in GL molecules, potentially accompanied by the release of the sulfate group. Moreover, we reveal that beta-glucosidases BGLU28 and BGLU30 are the major myrosinases that initiate sulfur reallocation by hydrolyzing particular GL species, conferring sulfur deficiency tolerance in A. thaliana, especially during early development. The results delineate the physiological function of GL as a sulfur reservoir, in addition to their well-known functions as defense chemicals. Overall, our findings demonstrate the bidirectional interaction between primary and specialized metabolism, which enhances our understanding of the underlying metabolic mechanisms via which plants adapt to their environments.

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Section: Resultssupporting

confidence: 83%

Retrograde sulfur flow from glucosinolates to cysteine in Arabidopsis thaliana

Sugiyama

Kuwahara

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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“…Compared with NAC, OTC belongs to a more stable Cys donor that seals both the amino and the sulfydryl groups of Cys ( Figure 5 ), avoiding of the spontaneous formation of disulfide linkage induced by free sulfydryl group [ 76 ]. In vitro , bioactivities of OTC are inferior to those of NAC [ 91 ], but in vivo or in cells that express 5-oxo- L -prolinase, OTC can be converted into Cys to elicit relevant bioactivities, due to its extremely similar structure with 5-oxo-proline, a natural substrate of 5-oxo- L -prolinase ( Figure 5 ) [ 92 ]. OTC shows favorable safety properties [ 93 ] as well as diverse physiological and pharmacological effects, such as GSH-boosting capability, antioxidant and anti-inflammatory functions, hepatic and cardiac protections, as well as anti-ischemic stroke effect ( Table 2 ).…”

Section: Cys and Its Donorsmentioning

confidence: 99%

Cysteine Donor-Based Brain-Targeting Prodrug: Opportunities and Challenges

Wang

et al. 2022

Oxidative Medicine and Cellular Longevity

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Overcoming blood-brain barrier (BBB) to improve brain bioavailability of therapeutic drug remains an ongoing concern. Prodrug is one of the most reliable approaches for delivering agents with low-level BBB permeability into the brain. The well-known antioxidant capacities of cysteine (Cys) and its vital role in glutathione (GSH) synthesis indicate that Cys-based prodrug could potentiate therapeutic drugs against oxidative stress-related neurodegenerative disorders. Moreover, prodrug with Cys moiety could be recognized by the excitatory amino acid transporter 3 (EAAT3) that is highly expressed at the BBB and transports drug into the brain. In this review, we summarized the strategies of crossing BBB, properties of EAAT3 and its natural substrates, Cys and its donors, and Cys donor-based brain-targeting prodrugs by referring to recent investigations. Moreover, the challenges that we are faced with and future research orientations were also addressed and proposed. It is hoped that present review will provide evidence for the pursuit of novel Cys donor-based brain-targeting prodrug.

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“…The enzyme required adenosine triphosphate (ATP), magnesium cations and potassium chloride for the cleavage of l‐ 2‐oxothiazolidine‐4‐carboxylic acid ( XI ). The enzyme also cleaved 5‐oxo‐ l‐ proline to l‐ glutamic acid and, therefore, it can be considered to be a β ‐5‐oxo‐ l‐ prolinase (Mochizuki ).…”

Section: Miscellaneous Enzymes and Other High‐value Bioproductsmentioning

confidence: 99%

Enzymes and bioproducts produced by the ascomycete fungus Paecilomyces variotii

2015

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Summary Due its innate ability to produce extracellular enzymes which can provide eco‐friendly solutions for a variety of biotechnological applications, Paecilomyces variotii is a potential source of industrial bioproducts. In this review, we report biotechnological records on the biochemistry of different enzymes produced by the fermentation of the P. variotii fungus, including tannases, phytases, cellulases, xylanases, chitinases, amylases and pectinases. Additionally, the main physicochemical properties which can affect the enzymatic reactions of the enzymes involved in the conversion of a huge number of substrates to high‐value bioproducts are described. Despite all the background information compiled in this review, more research is required to consolidate the catalytic efficiency of P. variotii, which must be optimized so that it is more accurate and reproducible on a large scale.

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Purification and characterization of 5-oxo- l -prolinase from Paecilomyces varioti F-1, an ATP-dependent hydrolase active with l -2-oxothiazolidine-4-carboxylic acid

Cited by 6 publications

References 9 publications

Retrograde sulfur flow from glucosinolates to cysteine in Arabidopsis thaliana

Retrograde sulfur flow from glucosinolates to cysteine in Arabidopsis thaliana

Cysteine Donor-Based Brain-Targeting Prodrug: Opportunities and Challenges

Enzymes and bioproducts produced by the ascomycete fungus Paecilomyces variotii

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