Purification and characterisation of glutathione reductase from scorpionfish
            <i>(scorpaena porcus)</i>
            and investigation of heavy metal ions inhibition

IŞIK, Kübra; Soydan, Ercan

doi:10.1080/14756366.2023.2167078

Cited by 3 publications

(1 citation statement)

References 43 publications

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“…It is responsible for maintaining the reduced state of GSH, a critical molecule involved in protecting cells from oxidative stress. [28] GR catalyzes the reduction of glutathione disulfide (GSSG) using NADPH. The stability of the GSH/GSSG ratio in the cell environment is the main objective of the GR enzyme-catalyzed process.…”

Section: Effects Of Methotrexate and Morin On Enzyme Activitiesmentioning

confidence: 99%

The effects of morin and methotrexate on pentose phosphate pathway enzymes and GR/GST/TrxR enzyme activities: An in vivo and in silico study

Caglayan,

Temel,

Türkeş

et al. 2023

Archiv der Pharmazie

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In this study, the mechanisms by which the enzymes glucose‐6‐phosphate dehydrogenase (G6PD), 6‐phosphogluconate dehydrogenase (6PGD), glutathione reductase (GR), glutathione‐S‐transferase (GST), and thioredoxin reductase (TrxR) are inhibited by methotrexate (MTX) were investigated, as well as whether the antioxidant morin can mitigate or prevent these adverse effects in vivo and in silico. For 10 days, rats received oral doses of morin (50 and 100 mg/kg body weight). On the fifth day, a single intraperitoneal injection of MTX (20 mg/kg body weight) was administered to generate toxicity. Decreased activities of G6PD, 6PGD, GR, GST, and TrxR were associated with MTX‐related toxicity while morin treatment increased the activity of the enzymes. The docking analysis indicated that H‐bonds, pi–pi stacking, and pi–cation interactions were the dominant interactions in these enzyme‐binding pockets. Furthermore, the docked poses of morin and MTX against GST were subjected to molecular dynamic simulations for 200 ns, to assess the stability of both complexes and also to predict key amino acid residues in the binding pockets throughout the simulation. The results of this study suggest that morin may be a viable means of alleviating the enzyme activities of important regulatory enzymes against MTX‐induced toxicity.

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Section: Effects Of Methotrexate and Morin On Enzyme Activitiesmentioning

confidence: 99%

The effects of morin and methotrexate on pentose phosphate pathway enzymes and GR/GST/TrxR enzyme activities: An in vivo and in silico study

Caglayan,

Temel,

Türkeş

et al. 2023

Archiv der Pharmazie

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The effects of ketoprofen and meloxicam on oxidative stress through the glutathione pathway after ketamine-xylazine anesthesia and ulcer induction in rats: A comparative study

Sheverini,

Khezrian,

Shojaeian

2024

Veterinary and Animal Science

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Purification of Glutathione Reductase Enzyme from Whiting Fish Gill Tissue and Investigation of Metal Inhibition

DUDAK,

IŞIK,

MİTROVİCA

et al. 2023

Black Sea Journal of Agriculture

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Pollution in the seas basically accumulates in marine organisms. Heavy metal residues in aquatic ecosystems can pass through food and cause toxic effects and accumulations in human health. Glutathione reductase (GR), which is among the basic enzymes, has an important place in the suppression of stress in the cell. In this study, glutathione reductase enzyme from whiting fish gill tissue was partially purified for the first time in the literature and the effects of heavy metal compounds on enzyme activity were determined. The purification process was carried out in three stages as homogenate preparation, ammonium sulfate precipitation and also dialysis. In conclusion the study, optimum level pH 7.0, optimal substrate concentration 2 mM NADPH and optimum buffer 150 mM KH2PO4 were determined. After partial purification, the inhibition effects of Cd+2, Ni+2, Zn+2 as heavy metal ions were investigated. The IC50 levels of heavy metals were calculated as 20.17 µM, 33.7 µM and 59.31 µM, respectively.

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Purification and characterisation of glutathione reductase from scorpionfish (scorpaena porcus) and investigation of heavy metal ions inhibition

Cited by 3 publications

References 43 publications

The effects of morin and methotrexate on pentose phosphate pathway enzymes and GR/GST/TrxR enzyme activities: An in vivo and in silico study

The effects of morin and methotrexate on pentose phosphate pathway enzymes and GR/GST/TrxR enzyme activities: An in vivo and in silico study

The effects of ketoprofen and meloxicam on oxidative stress through the glutathione pathway after ketamine-xylazine anesthesia and ulcer induction in rats: A comparative study

Purification of Glutathione Reductase Enzyme from Whiting Fish Gill Tissue and Investigation of Metal Inhibition

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