Purification and characterisation of aquamarine blue pigment from the shells of abalone (Haliotis discus hannai Ino)

Cai, Zhi-Xing; Wu, Jiulin; Chen, Li; Guo, Wei; Li, Jianhua; Wang, Jiabin; Zhang, Qiqing

doi:10.1016/j.foodchem.2011.03.006

Cited by 17 publications

(17 citation statements)

References 17 publications

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“…[23] The absorption peaks at 1469 cm À 1 , 2847 cm À 1 and 2920 cm À 1 indicated a long CH 2 chain in the molecular structure. [24] The absorp-tion peaks at 1021 cm À 1 , 1632 cm À 1 and 1658 cm À 1 were attributed to the stretching vibrations of CÀ O, C=C and C=O, [25] respectively. Therefore, a long CH 2 chain and the moieties of C=O, CÀ O, C=C and NÀ H were indicated in the structure of PP-Z (PP-Z).…”

Section: Ft-ir Analysismentioning

confidence: 99%

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Chemical Composition, Security and Bioactivity of the Red Pigment from Penicillium purpurogenum Li‐3

Jin

Zhang

Cao

et al. 2018

Chemistry & Biodiversity

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The chemical composition, security and bioactivity of pigments from Penicillium purpurogenum Li-3 strain screened by our group were firstly studied in this work. DPPH and the filter disc diffusion method were used to determine the biological activities of the red pigments. The pigment was characterized by UV/VIS, FT-IR, NMR and UPLC-Q-TOF-MS. HPLC/MS was used to detect mycotoxins (citrinin) in fermentation broth. An acute toxicity was detected in the embryos of zebrafish. As a consequence, the crude red pigment from the AcOEt fraction showed better DPPH scavenge capacity and antibacterial activity. Spectroscopic (UV, FT-IR, C-NMR) and UPLC-Q-TOF-MS analysis revealed that the Penicillium purpurogenum Li-3 red pigment (RPs) was monascus-like pigment and its molecular weight was 439.1997. Moreover, the red pigment was shown to be weak cytotoxic against the zebrafish embryos. The yield of the red pigment increased 69 % under optimized culture conditions. These outstanding properties will enlarge the application of RPs for natural food additives, new antioxidant and antibacterial drug development.

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Section: Ft-ir Analysismentioning

confidence: 99%

“…In the first stage, the cells were cultured for 50 h at 32°C for the rapid growth. The temperature was changed to 16,20,24 and 28°C in the second stage. The absorbance value of the red pigment was measured after 118 h of fermentation, and each treatment was repeated three times.…”

Section: Pigment Optimizationmentioning

confidence: 99%

Chemical Composition, Security and Bioactivity of the Red Pigment from Penicillium purpurogenum Li‐3

Jin

Zhang

Cao

et al. 2018

Chemistry & Biodiversity

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“…The biochemical nature of shell pigments was investigated by a number of researchers towards the end of the 1800s, reaching a peak in terms of number of studies in the mid-1900s (e.g. [ 5 , 13 – 24 ]). Older studies identified two main classes of shell pigments: melanins and tetrapyrroles (which include porphyrins and linear tetrapyrroles known as bile pigments or bilins) along with a number of other pigments that could not be fully characterised, some of which were complexed to proteins.…”

Section: Introductionmentioning

confidence: 99%

Identification of Shell Colour Pigments in Marine Snails Clanculus pharaonius and C. margaritarius (Trochoidea; Gastropoda)

et al. 2016

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Colour and pattern are key traits with important roles in camouflage, warning and attraction. Ideally, in order to begin to understand the evolution and ecology of colour in nature, it is important to identify and, where possible, fully characterise pigments using biochemical methods. The phylum Mollusca includes some of the most beautiful exemplars of biological pigmentation, with the vivid colours of sea shells particularly prized by collectors and scientists alike. Biochemical studies of molluscan shell colour were fairly common in the last century, but few of these studies have been confirmed using modern methods and very few shell pigments have been fully characterised. Here, we use modern chemical and multi-modal spectroscopic techniques to identify two porphyrin pigments and eumelanin in the shell of marine snails Clanculus pharaonius and C margaritarius. The same porphyrins were also identified in coloured foot tissue of both species. We use high performance liquid chromatography (HPLC) to show definitively that these porphyrins are uroporphyrin I and uroporphyrin III. Evidence from confocal microscopy analyses shows that the distribution of porphyrin pigments corresponds to the striking pink-red of C. pharaonius shells, as well as pink-red dots and lines on the early whorls of C. margaritarius and yellow-brown colour of later whorls. Additional HPLC results suggest that eumelanin is likely responsible for black spots. We refer to the two differently coloured porphyrin pigments as trochopuniceus (pink-red) and trochoxouthos (yellow-brown) in order to distinguish between them. Trochopuniceus and trochoxouthos were not found in the shell of a third species of the same superfamily, Calliostoma zizyphinum, despite its superficially similar colouration, suggesting that this species has different shell pigments. These findings have important implications for the study of colour and pattern in molluscs specifically, but in other taxa more generally, since this study shows that homology of visible colour cannot be assumed without identification of pigments.

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“…Stability of β‐CRX was studied by following the method of Cai et al . () with major modifications. The lyophilized β‐CRX powder was dissolved in

50 % (v / v)

aqueous ethanol for stability studies.…”

Section: Methodsmentioning

confidence: 99%

Effect of Selected Physico‐Chemical Factors on Bacterial Β‐Cryptoxanthin Degradation: Stability and Kinetic Study

Mitra

Samanta

Chaudhuri

et al. 2016

J Food Process Engineering

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Carotenoids are promising dietary supplements to human population due to its potential nutraceutical benefits. Their stability in food system is a big concern for food industry. In this study, stability of b-cryptoxanthin (b-CRX), a yellow colored pro-vitamin A xanthophyll, extracted from a previously isolated bacterium Kocuria marina DAGII, was studied under different processing conditions by first-order linear regression analysis and two-way ANOVA. The results indicated that the b-CRX resisted thermal treatments up to 508C with degradation rate constants (k) of 0.3731-0.8661 h 21, thermal death times (D values) of 6.1715-2.6586 h, a z value of 169.58C, an activation energy (E a ) of 13:27 kJ=mol, enthalpy ðDHÞ and entropy of activation (DS) of 11:80 kJ=mol and 20:2145 kJ=mol K, respectively, over the temperature range of 25-80 C. The resulting positive Gibbs free energy change (DG) indicated the non-spontaneous nature of the degradation reaction. Neutral to basic pH was favorable for the b-CRX processing. Light induced degradation was high ð83:14%Þ resulting into complete degradation of the b-CRX. Furthermore, addition of metallic ions significantly affected the b-CRX stability, however, it was stable in presence of Cu 21 , K 1 , oxidant ðH 2 O 2 Þ and reductant ðNa 2 SO 3 Þ. Till date there has been no detailed report on the degradation kinetics of b-CRX, obtained from microbial source. Hence considering all the tested parameters, this study is significant for using the extracted bacterial b-CRX as a dietary supplement. PRACTICAL APPLICATIONSb-CRX is a bioactive compound having great potential as a dietary supplement because of its therapeutic and immune-enhancing properties, especially the provitamin A activity, anti-oxidant and anti-cancer properties. But, the stability of b-CRX during food processing is a big concern due to the presence of polyunsaturated chains of conjugated double bonds. Thus, the present research work explores the stability of b-CRX, extracted from Kocuria marina DAGII, under different selected parameters that are important for processing of food. This study gives an idea about the effective b-CRX remaining after it is being exposed to high temperature, acidic pH, light and oxidizing environment. Results indicate the favorable conditions that should be maintained during the b-CRX processing for industrial application. Assessment of the chemical kinetics and application of the mathematical model will also provide information for designing an optimum process so that minimum degradation of b-CRX takes place.

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Purification and characterisation of aquamarine blue pigment from the shells of abalone (Haliotis discus hannai Ino)

Cited by 17 publications

References 17 publications

Chemical Composition, Security and Bioactivity of the Red Pigment from Penicillium purpurogenum Li‐3

Chemical Composition, Security and Bioactivity of the Red Pigment from Penicillium purpurogenum Li‐3

Identification of Shell Colour Pigments in Marine Snails Clanculus pharaonius and C. margaritarius (Trochoidea; Gastropoda)

Effect of Selected Physico‐Chemical Factors on Bacterial Β‐Cryptoxanthin Degradation: Stability and Kinetic Study

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