Purification and biochemical characterization of a detergent-stable keratinase from a newly thermophilic actinomycete Actinomadura keratinilytica strain Cpt29 isolated from poultry compost

A novel keratinase from Bacillus tequilensis strain Q7 with promising potential for the leather bating process

“…Likewise, the purified keratinases KERUS from Br. brevis strain US575 [15] and KERAK-29 from Actinomadura keratinilytica strain Cpt29 [33] were previously reported to be totally activated by Ca 2+ , Mn 2+ , and Mg 2+ but strongly inhibited by Cd 2+ , Ni 2+ , and Hg 2+ [15]. The increase in protease activity with Ca 2+ , Mn 2+ , and Mg 2+ indicated that the metallic ions exerted a protective effect against thermal denaturation, thereby playing a vital role in maintaining the active conformation of the enzyme at higher temperature.…”

A Review: Biodegradation and Applications of Keratin Degrading Microorganisms and Keratinolytic Enzymes, Focusing on Thermophiles and Thermostable Serine Proteases

“…BA7, Actinomadura keratinilytica Cpt29 and Nocardiopsis sp. TOA-1 (Williams et al, 1990;Lin et al, 1992;Letourneau et al, 1998;Wang and Shih, 1999;Korkmaz et al, 2003;Gousterova et al, 2005;Mitsuiki et al, 2006;Yoshioka et al, 2007;Habbeche et al, 2014).…”

Role of the durum wheat dehydrin in the function of proteases conferring salinity tolerance in Arabidopsis thaliana transgenic lines

“…One unit (U) of protease activity was defined as the amount of enzyme, which yielded 1 μg of tyrosine per minute under the experimental conditions used. Specific activities are expressed as U/mg of protein [15]. The determination of protease activity was monitored with and without specific inhibitors; the inhibitor used in the case of Cysteinyl protease is iodoacetamide at the concentration of 5 mM [16] and the inhibitor used in the case of Aspartyl proteases is pepstatine A at the concentration of 1 µg/ml [16].…”