Purification and Application of a Lipase from Penicillium expansum PED-03

Tang, Lanfang; Xia, Liming; Su, Min; Hua-ying, Guo

doi:10.1007/s12010-007-0043-2

Cited by 12 publications

(3 citation statements)

References 20 publications

(15 reference statements)

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“…The SDS-PAGE results strongly suggest that PCrL is a monomeric protein comparable to those previously reported for other lipases from Penicillium strains. The molecular mass of PCrL (28 kDa) is similar to that of the Penicillium expansum strain PED-03 [ 39 ]. Most of the known lipases from the genus Penicillium have been reported to have a molecular mass in the range of 25–43 kDa [ 22 , 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Purification, Biochemical and Kinetic Characterization of a Novel Alkaline sn-1,3-Regioselective Triacylglycerol Lipase from Penicilliumcrustosum Thom Strain P22 Isolated from Moroccan Olive Mill Wastewater

Hasnaoui

Dab

Mechri

et al. 2022

IJMS

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A novel extracellular lipase from a filamentous fungus Ascomycota strain, P22, was isolated from olive mill wastewater, then purified and characterized. This strain was identified as Penicillium crustosum Thom based on sequencing analyses. Penicillium crustosum Thom strain P22 lipase (PCrL) was purified 63-fold to homogeneity using ammonium sulfate precipitation and chromatography on a Q-Sepharose Fast Flow column, with a total yield of 34%. The purified PCrL had a molecular mass of 28 kDa, estimated by SDS-PAGE. The 20 NH2-terminal amino-acid residues showed a high degree of homology with those of other Penicillium lipases. The specific activity of PCrL at pH 9 and 37 °C were found to be 5000 and 10,000 U/mg on olive oil and trioctanoin emulsions, respectively. PCrL exhibited clear regioselectivity toward the sn-1 position of the surface-coated triglycerides which were esterified with α-eleostearic acid at the sn-1/3 position. PCrL was completely inhibited by 53 µM of Orlistat, 5 mM of phenylmethylsulfonylfluoride, and 2 mM of diiodopropyl fluorophosphate, suggesting that it belonged to the serine lipase family. PCrL showed high activity and stability in the presence of water-immiscible organic solvents, surfactant, and oxidizing agents, and showed considerable compatibility with commercial laundry detergents. Washing performance analysis revealed that it could effectively remove oil stains. Hence, PCrL has several attractive properties that make it a promising potential candidate for detergent formulations.

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Section: Discussionmentioning

confidence: 99%

Purification, Biochemical and Kinetic Characterization of a Novel Alkaline sn-1,3-Regioselective Triacylglycerol Lipase from Penicilliumcrustosum Thom Strain P22 Isolated from Moroccan Olive Mill Wastewater

Hasnaoui

Dab

Mechri

et al. 2022

IJMS

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“…The range of substrates converted by lipases can be learned from the following examples. In the work by Lianghua and coworkers, ibuprofen in R and S racemate reacts with 1-propanol in the presence of lipase from Penicillium expansum [23] while ( S )-ibuprofen n-propylester and ( R )-ibuprofen are the products. The reaction can be applied for the preparation or manufacturing of an optically pure isomer of ibuprofen.…”

Section: Lipases and Their Applicabilitymentioning

confidence: 99%

Biosensors and Bioassays Based on Lipases, Principles and Applications, a Review

Pohanka

2019

Molecules

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Lipases are enzymes responsible for the conversion of triglycerides and other esterified substrates, they are involved in the basic metabolism of a wide number of organisms, from a simple microorganism and to mammals. They also have broad applicability in many fields from which industrial biotechnology, the production of cleaning agents, and pharmacy are the most important. The use of lipases in analytical chemistry where it can serve as a part of biosensors or bioassays is an application of growing interest and has become another important use. This review is focused on the description of lipases chemistry, their current applications and the methods for their assay measurement. Examples of bioassays and biosensors, including their physical and chemical principles, performance for specific substrates, and discussion of their relevance, are given in this work.

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“…Thus, screening and using expression signals of other highly expressed genes in this fungus will definitely be a promising approach to further promote the performance of this engineered strain. P. expansum is an economical important filamentous fungus in consequence of its content of alkaline lipase, which is now widely used in many fields including biodiesel [31], detergent [32], leather [33], and fine chemicals [34]. Based on the newly characterized Ppel, the overexpression vector was constructed and after transformation and screening, recombinant strains that showed a significant increase (P < 0.05) in lipase yield were successfully obtained, which makes our research practically significant.…”

Section: Lipase Activity Assaymentioning

confidence: 99%

Characterization of the 5′ flanking region of lipase gene from Penicillium expansum and its application in molecular breeding

Zhang

Peng

et al. 2014

Biotech and App Biochem

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A major challenge for further promotion of lipase productivity in Penicillium expansum PE-12 is to find a suitable promoter that can function efficiently in this industrial strain. In this study, the 5' flanking region of P. expansum lipase (Ppel) containing a putative novel promoter sequence was characterized by fusing to β-glucuronidase (GUS) and subsequently introducing into P. expansum. As a result, all the transformants showed blue color quickly after incubation in GUS detection buffer, suggesting a strong promoter activity of this fragment. Glucose repression was identified for the promoter, whereas olive oil acted as a positive regulator. Facilitated by this novel promoter, P. expansum PE-12 was genetically modified, with an improved lipase yield, via a recombinant plasmid with P. expansum lipase gene (PEL) under the control of Ppel promoter and TtrpC terminator. The highest lipase yield among the modified strains could attain 2,100 U/mL, which is more than twofold of the previous industrial strain (900 U/mL). The engineered strain through molecular breeding method as well as this new promoter has great value in lipase industry.

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Purification and Application of a Lipase from Penicillium expansum PED-03

Cited by 12 publications

References 20 publications

Purification, Biochemical and Kinetic Characterization of a Novel Alkaline sn-1,3-Regioselective Triacylglycerol Lipase from Penicilliumcrustosum Thom Strain P22 Isolated from Moroccan Olive Mill Wastewater

Purification, Biochemical and Kinetic Characterization of a Novel Alkaline sn-1,3-Regioselective Triacylglycerol Lipase from Penicilliumcrustosum Thom Strain P22 Isolated from Moroccan Olive Mill Wastewater

Biosensors and Bioassays Based on Lipases, Principles and Applications, a Review

Characterization of the 5′ flanking region of lipase gene from Penicillium expansum and its application in molecular breeding

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