Purification and antimicrobial function of ubiquitin isolated from the gill of Pacific oyster, Crassostrea gigas

Seo, Jung-Kil; Lee, Min Jeong; Go, Hye‐Jin; Kim, Gun Do; Jeong, Hyun Do; Nam, Bo‐Hye; Park, Nam Gyu

doi:10.1016/j.molimm.2012.07.003

Cited by 47 publications

(49 citation statements)

References 55 publications

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“…Cg-Ubiquitin was bacteriostatic and did not permeabilize the bacterial membranes. No antifungal or hemolytic activities were observed for this polypeptide [67].…”

Section: Cg-ubiquitinmentioning

confidence: 84%

“…In oysters, the antimicrobial activity of ubiquitin has been recently reported [67]. Cg-Ubiquitin was purified from acidified gill extracts of C. gigas.…”

Section: Cg-ubiquitinmentioning

confidence: 99%

“…The polypeptide had a molecular weight of 8.4 kDa and the N-terminal region is identical to ubiquitin sequences reported from other species. Cg-Ubiquitin transcripts were found to be expressed at high level in the mantle tissue, and to a lesser extent in gills, digestive gland, adductor muscle and labial palps [67]. Cg-Ubiquitin is encoded as a 76 amino acid precursor fused to the ribosomal protein S27.…”

Section: Cg-ubiquitinmentioning

confidence: 99%

“…The precursor included a six amino acid motif and three conserved lysine at the functional sites, which might participate in the formation of the ubiquitineprotease complex. Cg-Ubiquitin also displays an 80-amino acid carboxyl ribosomal protein S27 extension with a classical zinc finger motif [67]. By homology modeling, Cg-Ubiquitin was deduced to display three secondary structural motifs, including three a-helices and four b-strands separated by 7 loop regions.…”

Section: Cg-ubiquitinmentioning

confidence: 99%

“…By homology modeling, Cg-Ubiquitin was deduced to display three secondary structural motifs, including three a-helices and four b-strands separated by 7 loop regions. The C-terminal region of the Cg-Ubiquitin mature polypeptide terminated with an Arg residue instead of a GlyeGly doublet, characteristic of ubiquitin molecules in other species [67].…”

Section: Cg-ubiquitinmentioning

confidence: 99%

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The new insights into the oyster antimicrobial defense: Cellular, molecular and genetic view

Bachère

Rosa

Schmitt

et al. 2015

Fish & Shellfish Immunology

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Oysters are sessile filter feeders that live in close association with abundant and diverse communities of microorganisms that form the oyster microbiota. In such an association, cellular and molecular mechanisms have evolved to maintain oyster homeostasis upon stressful conditions including infection and changing environments. We give here cellular and molecular insights into the Crassostrea gigas antimicrobial defense system with focus on antimicrobial peptides and proteins (AMPs). This review highlights the central role of the hemocytes in the modulation and control of oyster antimicrobial response. As vehicles for AMPs and other antimicrobial effectors, including reactive oxygen species (ROS), and together with epithelia, hemocytes provide the oyster with local defense reactions instead of systemic humoral ones. These reactions are largely based on phagocytosis but also, as recently described, on the extracellular release of antimicrobial histones (ETosis) which is triggered by ROS. Thus, ROS can signal danger and activate cellular responses in the oyster. From the current literature, AMP production/release could serve similar functions. We provide also new lights on the oyster genetic background that underlies a great diversity of AMP sequences but also an extraordinary individual polymorphism of AMP gene expression. We discuss here how this polymorphism could generate new immune functions, new pathogen resistances or support individual adaptation to environmental stresses.

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“…Cg-Ubiquitin was bacteriostatic and did not permeabilize the bacterial membranes. No antifungal or hemolytic activities were observed for this polypeptide [67].…”

Section: Cg-ubiquitinmentioning

confidence: 84%

“…In oysters, the antimicrobial activity of ubiquitin has been recently reported [67]. Cg-Ubiquitin was purified from acidified gill extracts of C. gigas.…”

Section: Cg-ubiquitinmentioning

confidence: 99%

Section: Cg-ubiquitinmentioning

confidence: 99%

Section: Cg-ubiquitinmentioning

confidence: 99%

Section: Cg-ubiquitinmentioning

confidence: 99%

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