Purification and Antibacterial Activity of Marine Actinomycetes against Human and Fish Pathogens

Tp, Kumari Pushpa Rani; Döss, A.

doi:10.4172/2155-9910.1000215

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…Actinobacteria telah dikenal sebagai bakteri penghasil senyawa bioaktif dengan beragam aktivitas biologis (Abdelfattah, Arai, & Ishibashi, 2016). Sebanyak dua pertiga dari 10.000 senyawa aktif biol ogis yang berasal dari mikroorganisme diproduksi oleh Actinobacteria (Kumari & Doss, 2016). Sebagian besar senyawa bioaktif yang dihasilkan merupakan antimikroba, di antaranya tetrasiklin, eritromisin, vancomisin, dan streptomycin (Subramani & Aalbersberg, 2012;Weber et al, 2015).…”

Section: Pendahuluanunclassified

Penapisan Actinobacteria Akuatik Penghasil Antibakteri Dari Ikan Bandeng (Chanos chanos) dan Belanak (Mugil cephalus) dengan Metode Double-Layer Diffusion

Kurnianto

Kusumaningrum

Lioe

2020

JPBKP

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Saluran pencernaan (terutama usus) ikan perairan estuaria merupakan salah satu ceruk lingkungan potensial Actinobacteria yang belum tereksplorasi. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi karakteristik morfologi Actinobacteria asal ikan bandeng (Chanos chanos) dan belanak (Mugil cephalus) serta mengevaluasi aktivitas antimikroba yang dihasilkannya. Penelitian ini diawali dengan mengambil usus ikan, kemudian digesta usus secara perlahan dipisahkan untuk dieksplorasi keberadaan Actinobacteria dengan menggunakan media isolasi selektif. Isolat yang diperoleh dikarakterisasi berdasarkan ciri makroskopik dan mikroskopik, serta dilakukan penapisan antibakteri awal menggunakan metode double-layer diffusion. Isolat dengan zona penghambatan terbaik dipilih untuk dilakukan produksi dan ekstraksi senyawa antibakteri, serta uji aktivitas antibakteri dengan metode difusi cakram terhadap bakteri uji Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa dan Escherichia coli. Sebanyak 44 isolat Actinobacteria telah diisolasi dari digesta usus ikan bandeng (Chanos chanos) dan belanak (Mugil cephalus) menggunakan media strach casein dan actinomycete isolation agar. Sebagian besar isolat yang diperoleh menunjukkan karakteristik morfologi genus Streptomyces sp., seperti koloni memiliki tekstur menyerupai serbuk, bertepung dan kasar, memiliki aerial miselium berwarna putih dan substrat miselium berwarna krim susu, serta memiliki bentuk rantai spora rectus-flexibilis. Proses penapisan antibakteri isolat Actinobacteria menunjukkan 22 isolat memiliki indeks penghambatan terhadap sedikitnya satu bakteri uji, dengan aktivitas terbaik ditunjukkan oleh isolat A-SCA-11. Uji antibakteri terhadap ekstrak kasar isolat A-SCA-11 menunjukkan aktivitas antibakteri berspektrum luas yang mampu menghambat seluruh bakteri uji dengan zona hambat tertinggi pada P. aeruginosa. AbstractThe gut of estuary fish is one of the potential novel niches of Actinobacteria that has not yet been explored. This study aimed to isolate and identify the morphological characteristics of Actinobacteria from milkfish (Chanos chanos) and blue-spot mullet fish (Mugil cephalus) and to evaluate the antibacterial activity produced. This research was started by taking the fish gut, and then the digesta were slowly separated to explore the presence of Actinobacteria using selective isolation media. The isolates obtained were characterized by macroscopic and microscopic characteristics, and antibacterial preliminary screening of isolates was performed using a double-layer diffusion method. The isolates with the best inhibition zone were selected for production and extraction of antibacterial compounds, and antibacterial activity tests using the disk-diffusion method against the test bacteria Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, and Escherichia coli. A total of 44 isolates of Actinobacteria have been isolated from the gut of fish using starch casein and actinomycete isolation agar. Most isolates showed morphological characteristics of the genus Streptomyces sp., such as colonies with a tough or powdery texture, antibacterial have white aerial mycelium and milk-cream substrate mycelium, and rectus-flexibilis spore chain. The antibacterial preliminary screening of Actinobacteria isolates showed 22 isolates had inhibitory index against at least one test bacterium, with the best activity indicated by A-SCA-11. Antibacterial test of A-SCA-11 crude extract showed broad-spectrum antibacterial activity that was able to inhibit all test bacteria with the highest inhibitory zone on P. aeruginosa.

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Section: Pendahuluanunclassified

Penapisan Actinobacteria Akuatik Penghasil Antibakteri Dari Ikan Bandeng (Chanos chanos) dan Belanak (Mugil cephalus) dengan Metode Double-Layer Diffusion

Kurnianto

Kusumaningrum

Lioe

2020

JPBKP

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“…Marine Actinobacteria, when compare with terrestrial Actinobacteria are highly active, can produce different types of bioactive compounds and also have differences in their characteristics. These differences are due to the harsh marine environmental condition of marine Actinobacteria [5]. Most marine Actinobacteria of medical and economical applications comes under the subclass Actinobacteridae.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Streptomyces Variabilis Strain-Vitumvb03 Isolated From Kanyakumari Marine Sediments

Shubha

Ushashi

Veena

et al. 2017

Asian J Pharm Clin Res

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Objective: This study was focused on an evaluation of antimicrobial activity of actinobacteria isolated from Kanyakumari marine sediments.Methods: This study includes sample collection from Kanyakumari beach, followed by isolation of marine Actinobacteria, screening for antibacterial activity and antifungal activity. Finally, the most active isolate was identified using 16S rRNA sequencing. Results:Marine Actinobacteria are one of the leading microbes of the ocean known for producing excellent secondary metabolites and having a broad spectrum of antimicrobial activity; hence, they are very important from an industrial point of view. The marine sediment sample used was collected from Kanyakumari beach, in the month of July, falling in the latitudinal range of 8.5074°N and in the longitude of 76.9730°E. A total of eight actinobacterial colonies were isolated by spread plate technique on Actinomycetes isolation agar, Kuster's agar, and starch casein agar. The isolate cultures were designated as UST1-UST8. Among them, UST3 showed a maximum zone of inhibition against clinical pathogens Salmonella typhi and Aspergillus niger with a zone of inhibition of 22 mm and 20 mm, respectively. In solvent extraction study, ethyl acetate and hexane was found as best solvents for extraction. Potential isolate UST3 was identified as Streptomyces variabilis using morphological studies and 16S rRNA sequencing. Potential strain was submitted as S. variabilis-VITUMVB03. Conclusion:Results obtained in this study concluded that marine Actinobacteria have broad-spectrum antimicrobial activity and can be used in the pharmaceutical industry.

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Purification and Antibacterial Activity of Marine Actinomycetes against Human and Fish Pathogens

Cited by 2 publications

References 6 publications

Penapisan Actinobacteria Akuatik Penghasil Antibakteri Dari Ikan Bandeng (Chanos chanos) dan Belanak (Mugil cephalus) dengan Metode Double-Layer Diffusion

Penapisan Actinobacteria Akuatik Penghasil Antibakteri Dari Ikan Bandeng (Chanos chanos) dan Belanak (Mugil cephalus) dengan Metode Double-Layer Diffusion

Antimicrobial Activity of Streptomyces Variabilis Strain-Vitumvb03 Isolated From Kanyakumari Marine Sediments

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