2012
DOI: 10.1590/s0100-54052012000200004
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Purificação parcial de inibidores de tripsina de sementes de Caesalpinia ferrea e Swartzia polyphylla e o efeito dos extratos protéicos sobre fungos fitopatogênicos

Abstract: Sementes de leguminosas apresentam alta concentração de inibidores de tripsina; estas proteínas estão envolvidas no metabolismo celular e também em mecanismos de defesa vegetal. A fim de confirmar ou não, a possível ação fungicida destas proteínas a partir de extratos de sementes de leguminosas arbóreas, o objetivo deste estudo foi detectar inibidores de tripsina em sementes de Caesalpinia ferrea (CfTI) e Swartzia polyphylla (SpTI) e testar os extratos contra os fungos fitopatogênicos Colletotrichum guaranicol… Show more

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Cited by 11 publications
(8 citation statements)
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“…Caesalpinia ferrea has several biological activities (Bariani et al, 2012). With respect to cytotoxicity in murine L929 fibroblast cells exposed for 48 h to C. ferrea extract and coated AgNP, the results demonstrated a dose-dependent increase in AgNP concentration, causing significant cell death for the C. ferrea extract only at the highest concentration of 1,000 μg mL −1 .…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Caesalpinia ferrea has several biological activities (Bariani et al, 2012). With respect to cytotoxicity in murine L929 fibroblast cells exposed for 48 h to C. ferrea extract and coated AgNP, the results demonstrated a dose-dependent increase in AgNP concentration, causing significant cell death for the C. ferrea extract only at the highest concentration of 1,000 μg mL −1 .…”
Section: Resultsmentioning
confidence: 99%
“…Martius is popularly referred to as ironwood or jucá, and the traditional pharmacological applications of shells, seeds, roots and fruits from this species are used to heal wounds and bruises (Cavalheiro et al, 2009; Bariani et al, 2012; Silva et al, 2015). Accordingly, anti-inflammatory (Araújo et al, 2014; Kobayashi et al, 2015), antifungal (Bariani et al, 2012), antihistaminic, antiallergic, anticoagulant and larvicidal activities (Cavalheiro et al, 2009), and antiproliferative, cytoprotective and antimutagenic effects have been shown (Silva et al, 2015). …”
Section: Introductionmentioning
confidence: 99%
“…In the analyses performed by Bariani et al (2012), trypsin inhibition did not significantly differ for different leaf extract concentrations from Caesalpinia ferrea and Swartzia polyphylla (1, 9, and 12 μg).…”
mentioning
confidence: 78%
“…The protein profile for Cb-R2 corresponded to the proteins from Cb-R2ꞌ and Cb-R3ꞌ; thus, the trypsin inhibitor was not present in the latter fractions. Bariani et al (2012) investigated trypsin inhibitors from C. ferrea and S. polyphylla protein extracts and also observed 3 different peaks (PI, PII, and PIII) after gel filtration chromatography using a Sephadex G-100 column, which yielded fractions with low trypsin inhibitory activity.…”
mentioning
confidence: 99%
“…Among the plant species of the Brazilian biome, Caesalpinia ferrea (Tul.) Martius is popularly referred to as ironwood or jucá, and the traditional pharmacological applications of shells, seeds, roots, and fruits from this species are used to heal wounds and bruises (Cavalheiro et al, 2009;Bariani et al, 2012;Silva et al, 2015). Accordingly, anti-inflammatory (Araújo et al, 2014;Kobayashi et al, 2015), antifungal (Bariani et al, 2012), antihistaminic, antiallergic, anticoagulant, and larvicidal activities (Cavalheiro et al, 2009)], and antiproliferative, cytoprotective, and antimutagenic effects have been shown (Silva et al, 2015).…”
Section: Introductionmentioning
confidence: 99%