Purging in autologous hematopoietic stem cell transplantation using adenosine triphosphate (ATP) and 4-hydroperoxycyclophosphamide (4-HC)

Hatta, Yoshihiro; Itoh, Takuya; Baba, Masumi; Miyajima, Tsuyoshi; Shimojima, Hiroyuki; Sawada, Umihiko; Horie, Takashi

doi:10.1016/s0145-2126(01)00164-3

Cited by 7 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Table 4 (Additional file 9 )). Interesting, ATP has been reported as treatment target of murine leukemic cells in vitro to reduce the number of leukemic clonogenic cells [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

Mechanism-anchored profiling derived from epigenetic networks predicts outcome in acute lymphoblastic leukemia

et al. 2009

View full text Add to dashboard Cite

Background: Current outcome predictors based on "molecular profiling" rely on gene lists selected without consideration for their molecular mechanisms. This study was designed to demonstrate that we could learn about genes related to a specific mechanism and further use this knowledge to predict outcome in patients -a paradigm shift towards accurate "mechanismanchored profiling". We propose a novel algorithm, PGnet, which predicts a tripartite mechanismanchored network associated to epigenetic regulation consisting of phenotypes, genes and mechanisms. Genes termed as GEMs in this network meet all of the following criteria: (i) they are co-expressed with genes known to be involved in the biological mechanism of interest, (ii) they are also differentially expressed between distinct phenotypes relevant to the study, and (iii) as a biomodule, genes correlate with both the mechanism and the phenotype.

show abstract

“…Table 4 (Additional file 9 )). Interesting, ATP has been reported as treatment target of murine leukemic cells in vitro to reduce the number of leukemic clonogenic cells [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

Mechanism-anchored profiling derived from epigenetic networks predicts outcome in acute lymphoblastic leukemia

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Earlier studies indicated that extracellular ATP can increase the cellular accumulation of cytotoxic substances and can thereby increase the sensitivity of tumor cells towards those substances [23][24][25][26][27] . This study has been carried out to examine the potential to use ATP in addition to cisplatin to boost cisplatin activity in ovarian carcinoma cells.…”

Section: Discussionmentioning

confidence: 99%

“…Among the various other modes of action discussed, increasing the permeation of chemotherapeutic agents was cited as the main reason for sensitization of tumor cells by ATP [23][24][25][26][27] . The underlying mechanism is believed to be due to the interaction of ATP with the purinergic receptors on the cell surface, which opens the ion channels resulting in the rapid influx of Na + and Ca 2+ and efflux of K + and finally leading to membrane depolarization.…”

Section: Introductionmentioning

confidence: 99%

Effect of Extracellular ATP on Cisplatin-Induced Cytotoxicity in Human Ovarian Carcinoma Cells

Rotte

Garmann²,

Buβ³

et al. 2010

Chemotherapy

View full text Add to dashboard Cite

Background: Cisplatin resistance has been mainly associated with decreased cellular accumulation and increased intracellular glutathione (GSH) levels. ATP is known to increase the membrane permeability of cells and to decrease intracellular GSH levels. Our study aimed at using extracellular ATP to sensitize ovarian carcinoma cells towards cisplatin. Methods: The MTT assay was used to determine the EC₅₀ of cisplatin in the human ovarian carcinoma cell line A2780 and its cisplatin-resistant variant A2780cis. Intracellular platinum concentrations were determined using flameless atomic absorption spectrometry. Results: Preincubation and concomitant incubation with ATP did not alter the EC₅₀ of cisplatin. The presence of 100 µM ATP along with cisplatin decreased cell survival in A2780 but not in A2780cis cells. Extracellular ATP did not increase the cellular accumulation of cisplatin in both A2780 and A2780cis cells. Conclusion: The presence of extracellular ATP during treatment with cisplatin leads to additive cytotoxicity in the sensitive A2780 but not in cisplatin-resistant A2780cis cells.

show abstract

“…A positive effect exerted against tumors has been obtained through the pharmacological treatment of cancer cells with ATP. This maneuver is aimed at increasing the up-take of cytotoxic drugs by P2X7-overexpressing transformed cells, potentiating for example the cytotoxic effects of 4-hydroperoxycyclophosphamide (4HC) towards leukemia cells, minimally influencing viability of normal hematopoietic stem cells [73]. On the same line, ATP administration magnifies the effect of etoposide (VP16) treatment in lung adenocarcinoma cell lines, allowing elimination of cancer cells, another ATP-linked important application useful for purging leukemic cells before autologous stem cell transplantation [73][74].…”

Section: Tumor Cells and Extracellular Atp: A Complex Relationshipmentioning

confidence: 99%

A Purinergic Trail for Metastases

Ferrari

Malavasi²,

Antonioli

2017

Trends in Pharmacological Sciences

View full text Add to dashboard Cite

Purging in autologous hematopoietic stem cell transplantation using adenosine triphosphate (ATP) and 4-hydroperoxycyclophosphamide (4-HC)

Cited by 7 publications

References 41 publications

Mechanism-anchored profiling derived from epigenetic networks predicts outcome in acute lymphoblastic leukemia

Mechanism-anchored profiling derived from epigenetic networks predicts outcome in acute lymphoblastic leukemia

Effect of Extracellular ATP on Cisplatin-Induced Cytotoxicity in Human Ovarian Carcinoma Cells

A Purinergic Trail for Metastases

Contact Info

Product

Resources

About