Purge NMR: Effective and easy solvent suppression

Simpson, André J.; Brown, Sarah Anne

doi:10.1016/j.jmr.2005.05.008

Cited by 193 publications

(154 citation statements)

References 9 publications

Supporting

Mentioning

154

Contrasting

Order By: Relevance

“…Samples were then transferred into 5- H NMR experiments were performed using Presaturation Using Relaxation Gradients and Echoes (PURGE) water suppression, 128 scans, a recycle delay of 3 s, and 16 K time domain points. [29] Spectra were apodised through multiplication with an exponential decay corresponding to 0.3-Hz line broadening in the transformed spectrum, and a zero filling factor of 2. All spectra were manually phased and calibrated to the DSS internal reference methyl singlet, set to a chemical shift (d) of 0.00 ppm.…”

Section: à2mentioning

confidence: 99%

1H NMR-based metabolomic observation of a two-phased toxic mode of action in Eisenia fetida after sub-lethal phenanthrene exposure

et al. 2011

View full text Add to dashboard Cite

Environmental context. Phenanthrene is a persistent soil contaminant, whose toxic mode of action in earthworms has not been fully examined. We adopt a metabolomics approach, using 1 H nuclear magnetic resonance (NMR) spectroscopy, to measure the response of earthworms to sub-lethal phenanthrene exposure. The results indicate that NMR-based metabolomics may be used to monitor responses to sub-lethal levels of contaminants and to delineate their toxic mode of action. Abstract.1 H NMR-based metabolomics was used to examine the response of the earthworm Eisenia fetida to sublethal phenanthrene exposure. E. fetida were exposed via contact tests to six sub-lethal (below the measured LC 50 of 1.6 mg cm À2) concentrations of phenanthrene (0.8-0.025 mg cm À2 ) for 48 h. Multivariate statistical analysis of the 1 H NMR spectra of earthworm tissue extracts revealed a two-phased mode of action (MOA). At exposures below 1/16th of the LC 50 , the MOA was characterised by a linear correlation between the metabolic response and exposure concentration. At exposures !1/16th of the LC 50 , the metabolic response to phenanthrene appeared to plateau, indicating a distinct change in the MOA. Further data analysis suggested that alanine, lysine, arginine, isoleucine, maltose, ATP and betaine may be potential indicators for sub-lethal phenanthrene exposure. Metabolite variation was also found to be proportional to the exposure concentration suggesting that NMR-based earthworm metabolomics is capable of elucidating concentrationdependent relationships in addition to elucidating the MOA of sub-lethal contaminant-exposure.

show abstract

Section: à2mentioning

confidence: 99%

1H NMR-based metabolomic observation of a two-phased toxic mode of action in Eisenia fetida after sub-lethal phenanthrene exposure

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Samples were analysed using a Bruker Avance 500 MHz NMR spectrometer equipped with a 1 H-BB-13 C 5 mm, triple resonance broadband inverse probe at 298 K. 1-D solution state 1 H NMR experiments were performed with 256 scans, a recycle delay of 3 s, 32 768 time domain points, and an acquisition time of 1.6 s. Solvent suppression was achieved by presaturation utilising relaxation gradients and echoes. [18] Spectra were apodised through multiplication with an exponential decay corresponding to 1-Hz line broadening, and a zero-filling factor of 2. Diffusionedited (DE) experiments were performed using a bipolar pulse longitudinal encode-decode sequence.…”

Section: Nmr Spectroscopic Analysismentioning

confidence: 99%

Composition of dissolved organic matter within a lacustrine environment

McCaul¹,

Sutton²,

Simpson³

et al. 2011

Environ. Chem.

View full text Add to dashboard Cite

Environmental context. Freshwater dissolved organic matter is a complex chemical mixture central to many environmental processes, including carbon and nitrogen cycling. Questions remain, however, as to its chemical characteristics, sources and transformation mechanisms. We studied the nature of dissolved organic matter in a lake system and found that it is influenced by anthropogenic activities. Human activities can therefore influence the huge amounts of carbon sequestered in lakes as dissolved organic matter.Abstract. Freshwater dissolved organic matter (DOM) is a complex mixture of chemical components that are central to many environmental processes, including carbon and nitrogen cycling. However, questions remain as to its chemical characteristics, sources and transformation mechanisms. Here, we employ 1-and 2-D nuclear magnetic resonance (NMR) spectroscopy to investigate the structural components of lacustrine DOM from Ireland, and how it varies within a lake system, as well as to assess potential sources. Major components found, such as carboxyl-rich alicyclic molecules (CRAM) are consistent with those recently identified in marine and freshwater DOM. Lignin-type markers and protein/peptides were identified and vary spatially. Phenylalanine was detected in lake areas influenced by agriculture, whereas it is not detectable where zebra mussels are prominent. The presence of peptidoglycan, lipoproteins, large polymeric carbohydrates and proteinaceous material supports the substantial contribution of material derived from microorganisms. Evidence is provided that peptidoglycan and silicate species may in part originate from soil microbes.

show abstract

“…While there are certainly many more solvent suppression techniques reported in the literature, [2,4,5,16,17] the pulse sequences described in the Experimental section were conceived on an exemplary basis to inspect the effects of three main elements commonly used in solvent suppression: saturation, selective pulses (soft or composite), and gradient filters. Furthermore, the examined solvent suppression pulse sequences cover a broad range of complexities with associated hardware requirements, i.e.…”

Section: Resultsmentioning

confidence: 99%

T₂ relaxation measurement with solvent suppression and implications to solvent suppression in general

Hoffmann

Sobstyl

Badali

2009

Magnetic Reson in Chemistry

View full text Add to dashboard Cite

A number of suppression pulse sequences including Excitation Sculpting and WATERGATE were incorporated into the standard Carr-Purcell-Meiboom-Gill (CPMG) program for T 2 measurement and experimentally evaluated. The chosen suppression schemes were of varying complexity encompassing pulse program elements, such as presaturation, gradients, and selective pulses, which are typically utilized for solvent suppression. The quality of the spectral data and the accuracy of T 2 measurements of the investigated suppression schemes were evaluated using three aqueous samples with increasing proton content in the water solvent,

show abstract

Purge NMR: Effective and easy solvent suppression

Cited by 193 publications

References 9 publications

1H NMR-based metabolomic observation of a two-phased toxic mode of action in Eisenia fetida after sub-lethal phenanthrene exposure

1H NMR-based metabolomic observation of a two-phased toxic mode of action in Eisenia fetida after sub-lethal phenanthrene exposure

Composition of dissolved organic matter within a lacustrine environment

T₂ relaxation measurement with solvent suppression and implications to solvent suppression in general

Contact Info

Product

Resources

About

Purge NMR: Effective and easy solvent suppression

Cited by 193 publications

References 9 publications

1H NMR-based metabolomic observation of a two-phased toxic mode of action in Eisenia fetida after sub-lethal phenanthrene exposure

1H NMR-based metabolomic observation of a two-phased toxic mode of action in Eisenia fetida after sub-lethal phenanthrene exposure

Composition of dissolved organic matter within a lacustrine environment

T2 relaxation measurement with solvent suppression and implications to solvent suppression in general

Contact Info

Product

Resources

About

T₂ relaxation measurement with solvent suppression and implications to solvent suppression in general