Pure red cell aplasia in a case of Ph negative BCR/ABL rearranged CML with t(12;14)(q23;p11)

Lion, Thomas; Gaiger, Alexander; Henn, Traudl; Geißler, Klaus; Lechner, Klaus; Haas, Oskar A.

doi:10.1016/0165-4608(91)90170-y

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…Although in most of the cases bcr/abl transcript has shown persistence, there are some publications about RT-PCR negativity of chimeric mRNA in the patients that are receiving long-term treatment of recombinant interferon-␣ [17][18][19]. In conclusion, our data together with other previously published studies [14][15][16] suggest that interferon-␣ has reduced the fusion transcript product of Ph clone to the levels which is not detectable even RT-PCR analysis. Whether this corresponds to true eradication of Ph clone or rather to its persistence at low level is at present unknown.…”

Section: Discussionsupporting

confidence: 77%

“…In conclusion, our data together with other previously published studies [14][15][16] suggest that interferon-␣ has reduced the fusion transcript product of Ph clone to the levels which is not detectable even RT-PCR analysis. Whether this corresponds to true eradication of Ph clone or rather to its persistence at low level is at present unknown.…”

supporting

confidence: 60%

“…There were no detectable bands in results of three patients (#6, #7, and #20). However, they have shown bcr/abl transcript that was not detectable by southern blot but was detected by RT-PCR analysis which is the most sensitive technique [14][15][16]. In addition, it has been shown that during the follow-up period RT-PCR results become negative after 20, 43, and 44 months of interferon treatment.…”

Section: Discussionmentioning

confidence: 99%

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Detection of BCR/ABL gene rearrangement and the elimination of rearranged clone in chronic myelocytic leukemia patients

Eren¹,

Aytac²,

Tetik³

et al. 2000

Am. J. Hematol.

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Cytogenetic and molecular studies were performed on 20 interferon-alpha receiving Turkish chronic myelocytic leukemia patients. Four different restriction endonucleases and bcr-G probe were used for southern blot analysis to detect rearrangements of the bcr gene. The RT-PCR method was also applied to detect chimeric bcr/abl mRNA. Seventeen patients showed a chromosomal break within the 5.8 kb M-bcr region by southern blot analysis while three cases out of 20 have not shown any rearrangement. These three cases were further analysed by RT-PCR and they were also found to be carrying the Philadelphia translocation (Ph). However, in four years of follow-up this RT-PCR positivity has disappeared, which suggests an elimination of Ph clone with prolonged interferon-alpha treatment.

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Section: Discussionsupporting

confidence: 77%

supporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

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Detection of BCR/ABL gene rearrangement and the elimination of rearranged clone in chronic myelocytic leukemia patients

Eren¹,

Aytac²,

Tetik³

et al. 2000

Am. J. Hematol.

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“…18 RNA was dissolved in 25 l diethyl-pyrocarbonate-treated water and used directly for reverse transcription. [19][20][21][22] AmpliTaq DNA Polymerase (Perkin Elmer Cetus, Norwalk, CT, USA), 2.5 mm MgCl 2 and 20 pmol of each primer in a total reaction volume of 50 l were used. Amplification was performed in a Perkin Elmer thermocycler during one or, when necessary, two subsequent rounds of PCR, for 35 cycles.…”

mentioning

confidence: 99%

Prognostic significance of WT1 gene expression at diagnosis in adult de novo acute myeloid leukemia

et al. 1997

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We examined the presence of WT1-specific mRNA in bone marmyeloid leukemia (AML) has been demonstrated. Several row samples of 125 patients with de novo acute myeloid leukeauthors [10][11][12][13][14][15][16][17] have shown that in the majority of AML cases mia at diagnosis by two-step RT-PCR. The sensitivity of the WT1 is expressed. In view of these findings it was of interest assay was 1:100 (first step) and 1:10 000 (second step), to see whether WT1 positivity or negativity is associated with respectively. WT1-specific mRNA was detected in 73% of specific known features of AML or might be useful as a prog- to the French-American-British (FAB) Acute Leukemia Coop-

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“…Thus, cellular analysis can either be performed at the system level to measure the average response from the entire cell population or at the single‐cell level by revealing the hierarchical and interconnected attributes of the highly heterogeneous cell subsets. The former approach, including conventional enzyme‐linked immunosorbent assays (ELISA), quantitative polymerase chain reaction (qPCR), and other common biological assays, do provide exceptional insights into life science and medical diagnosis . However, these methods obscure important information regarding the specific phenotypes and status of the cells, and fail to unravel the casual events and the basic nature of cellular interactions among the discriminated cell types.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances and Perspectives in Microfluidics‐Based Single‐Cell Biosensing Techniques

Brimmo

Qasaimeh

et al. 2017

Small Methods

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Single‐cell analyses of secretory proteins are essential to fully understand cellular functional heterogeneity and unravel the underlying mechanisms of intercellular signaling and interactions. Retrieving dynamic information of protein secretion at single‐cell resolution reflects the precise, real‐time functional states of individual cells in physiological processes. Such measurements remain very challenging in single‐cell analysis, which requires highly integrated systems capable of performing on‐chip single‐cell isolation and subsequent real‐time protein detection. Here, recent advances in microfluidics‐based single‐cell manipulation and emerging approaches for label‐free single‐cell biosensing are reviewed. The advantages and limitations of these technologies are summarized and challenges to establish the integrated microfluidic biosensing systems for real‐time single‐cell secretomics are discussed. Recent efforts on integrated platforms for on‐chip single‐cell protein assays are highlighted and some perspectives on future directions in this field are provided.

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Pure red cell aplasia in a case of Ph negative BCR/ABL rearranged CML with t(12;14)(q23;p11)

Cited by 7 publications

References 18 publications

Detection of BCR/ABL gene rearrangement and the elimination of rearranged clone in chronic myelocytic leukemia patients

Detection of BCR/ABL gene rearrangement and the elimination of rearranged clone in chronic myelocytic leukemia patients

Prognostic significance of WT1 gene expression at diagnosis in adult de novo acute myeloid leukemia

Recent Advances and Perspectives in Microfluidics‐Based Single‐Cell Biosensing Techniques

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