Pure Protein Bilayers and Vesicles from Native Fungal Hydrophobins

Hähl, Hendrik; Vargas, Jose Nabor; Griffo, Alessandra; Laaksonen, Päivi; Szilvay, Géza R.; Lienemann, Michael; Jacobs, Karin; Seemann, Ralf; Fleury, Jean‐Baptiste

doi:10.1002/adma.201602888

Cited by 27 publications

(40 citation statements)

References 47 publications

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“…The results showed no significant upregulation of autophagy marker genes associated with high hfb gene activity (aerial hyphae) Taken together, the results of the above analyses indicated that HFBs first accumulated in lipid bodies that were internalized in vacuoles and likely gave rise to VMSs. In VMSs, HFBs self-assembled in the hydrophobic/hydrophilic interface led to the lining up of VMS membranes and/or formation of HFB vesicles similar to the bilayer HFB1 vesicles reported by Hähl et al in a cell-free system 32 . In some cells, several VMSs containing HFB vesicles formed gigantic central tonoplasts, as observed in Fig.…”

Section: Hfbs Massively Accumulate In Aerial Hyphae Of Filamentous Fusupporting

confidence: 61%

Intracellular accumulation and secretion of hydrophobin-enriched vesicles aid the rapid sporulation of molds

Cai¹,

Zhao²,

Gao³

et al. 2020

Preprint

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Fungi can rapidly produce large amounts of spores suitable for aerial dispersal. The hydrophobicity of spores is provided by the unique amphiphilic and superior surface-active proteins - hydrophobins (HFBs) - that self-assemble at hydrophobic/hydrophilic interfaces and thus change surface properties. Using the HFB-enriched mold Trichoderma and the HFB-free yeast Pichia pastoris, we revealed a distinctive HFB secretory pathway that includes an intracellular accumulation of HFBs in lipid bodies (LBs) that can internalize in vacuoles. The resulting vacuolar multicisternal structures (VMS) are stabilized by HFB layers that line up on their surfaces. These HFB-enriched VMSs can move to the periplasm for secretion or become fused in large tonoplast-like organelles. The latter contributes to the maintenance of turgor pressure required for the erection of sporogenic structures and rapid HFB secretion by squeezing out periplasmic VMSs through the cell wall. Thus, HFBs are essential accessory proteins for the development of aerial hyphae and colony architecture.

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Section: Hfbs Massively Accumulate In Aerial Hyphae Of Filamentous Fusupporting

confidence: 61%

Intracellular accumulation and secretion of hydrophobin-enriched vesicles aid the rapid sporulation of molds

Cai¹,

Zhao²,

Gao³

et al. 2020

Preprint

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“…Ein auf die Doppelmembran gerichteter Flüssigkeitsstrom induziert die Bildung von geschlossenen Vesikeln. – a) verändert nach , b) nach , c) aus …”

Section: Rekordverdächtige Amphiphileunclassified

“…Weiterhin gelang, angeregt durch den Flüssigkeitsstrom aus einer feinen Kanüle, die Bildung stabiler, von Hydrophobin‐Membranen umgrenzter Vesikel mit einem Durchmesser von etwa 100 μm (Abbildung c). In solche Vesikel – mit hydrophiler Oberfläche – konnte das Protein Gramicidin A inseriert werden, das in der Proteinmembran genauso als Ionenkanal funktionierte wie in einer Lipidmembran .…”

Section: Rekordverdächtige Amphiphileunclassified

Die Hydrophobine der Pilze – Vielzweckproteine mit Anwendungspotential

Hille-Rehfeld¹

2019

Chemie in nserer Zeit

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Die Proteinfamilie der Hydrophobine, von Pilzen in bestimmten Stadien ihres Lebenszyklus massenhaft produzierte und aus den Zellen ausgeschleuste oberflächenaktive Substanzen, faszinieren Mikrobiologen gleichermaßen wie Materialwissenschaftler. Aufsehen erregen diese kleinen globulären Proteine vor allem durch ihre bemerkenswerte Fähigkeit, sich an Grenzflächen zwischen hydrophilen und hydrophoben Medien zu geordneten Schichten zu organisieren, die nur eine einzelne Moleküllage enthalten. Geschieht dies in wässriger Lösung, verringern sie deren Oberflächenspannung und stabilisieren Gasbläschen oder emulgierte Fett‐Tröpfchen. Auf Festkörper aufgelagert bewirken sie einen Wechsel der Oberflächenbeschaffenheit von hydrophil nach hydrophob oder umgekehrt. Diese Eigenschaften der Hydrophobine erklären nicht nur das gefürchtete Überschäumen von mit Pilzen kontaminierten alkoholischen Getränken, sie beflügeln auch Visionen bezüglich ihrer biotechnologischen Anwendung.

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“…Furthermore, this approach does not require high temperature, pressure, and voltage to fabricate fibers as is necessary for conventional methods . Additionally, the microfluidic platform can be applied to create other bioactive materials, such as particles, liposomes, droplets, and vesicles …”

Section: Introductionmentioning

confidence: 99%

“…[21][22][23][24] Additionally, the microfluidic platform can be applied to create other bioactive materials, such as particles, liposomes, droplets, and vesicles. [25,26] Using poly (ε-caprolactone) (PCL) fibrous scaffolds has two main advantages. PCL can be considered as a good candidate for long-term implantable devices due to its slow degradation rate compared to other synthetic polymers, like poly(lactic-coglycolic acid) (PLGA), making the microenvironment less acidic during the degradation process.…”

Section: Introductionmentioning

confidence: 99%

3D Microfibrous Scaffolds Selectively Promotes Proliferation and Glial Differentiation of Adult Neural Stem Cells: A Platform to Tune Cellular Behavior in Neural Tissue Engineering

Patel

Sharifi

Stroud

et al. 2018

Macromolecular Bioscience

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Biomaterials are essential for the development of innovative biomedical and therapeutic applications. Biomaterials‐based scaffolds can influence directed cell differentiation to improve cell‐based strategies. Using a novel microfluidics approach, poly (ε‐caprolactone) (PCL), is used to fabricate microfibers with varying diameters (3–40 µm) and topographies (straight and wavy). Multipotent adult rat hippocampal stem/progenitor cells (AHPCs) are cultured on 3D aligned PCL microfibrous scaffolds to investigate their ability to differentiate into neurons, astrocytes, and oligodendrocytes. The results indicate that the PCL microfibers significantly enhance proliferation of the AHPCs compared to control, 2D planar substrates. While the AHPCs maintained their multipotent differentiation capacity when cultured on the PCL scaffolds, there is a significant and dramatic increase in immunolabeling for astrocyte and oligodendrocyte differentiation when compared with growth on planar surfaces. Our results show a 3.5‐fold increase in proliferation and 23.4‐fold increase in astrocyte differentiation for cells on microfibers. Transplantation of neural stem/progenitor cells within a PCL microfiber scaffold may provide important biological and topographic cues that facilitate the survival, selective differentiation, and integration of transplanted cells to improve therapeutic strategies.

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Pure Protein Bilayers and Vesicles from Native Fungal Hydrophobins

Cited by 27 publications

References 47 publications

Intracellular accumulation and secretion of hydrophobin-enriched vesicles aid the rapid sporulation of molds

Intracellular accumulation and secretion of hydrophobin-enriched vesicles aid the rapid sporulation of molds

Die Hydrophobine der Pilze – Vielzweckproteine mit Anwendungspotential

3D Microfibrous Scaffolds Selectively Promotes Proliferation and Glial Differentiation of Adult Neural Stem Cells: A Platform to Tune Cellular Behavior in Neural Tissue Engineering

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