Pure Platelet-Rich Plasma and Supernatant of Calcium-Activated P-PRP Induce Different Phenotypes of Human Macrophages

Escobar, Gisselle; Escobar, Alejandro; Ascui, Gabriel; Tempio, Fabián; Ortíz, Mario A.; Pérez, Claudio; López, Mercedes

doi:10.2217/rme-2017-0122

Cited by 29 publications

(33 citation statements)

References 49 publications

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“…Platelet‐rich plasma holds an anti‐inflammatory effect on murine RAW 264.7 cells and primary macrophages and also induces the expression of ARG1 . Furthermore, PRP decreased the inflammatory response of human dendritic cells and macrophages stimulated with A. fumigatus and leucocyte‐depleted PRP increased the M2 marker mannose receptor CD206 in human macrophages . We thus extend previous observations obtained with platelet‐rich plasma but focusing mainly on the clinical approach using PRF membranes .…”

Section: Discussionsupporting

confidence: 79%

“…15,16 Furthermore, PRP decreased the inflammatory response of human dendritic cells and macrophages stimulated with A. fumigatus 17 and leucocyte-depleted PRP increased the M2 marker mannose receptor CD206 in human macrophages. 18 We thus extend previous observations obtained with platelet-rich plasma but focusing mainly on the clinical approach using PRF membranes. 16 We show here that PRF lysates produced by freeze-thawing the membranes 31 and PRF conditioned medium both decreased the inflammatory response of macrophages.…”

Section: Discussionsupporting

confidence: 71%

“…Platelet‐rich plasma decreased the inflammatory response of human monocyte‐derived dendritic cells and macrophages stimulated with Aspergillus fumigatus . Moreover, leukocyte‐depleted platelet‐rich plasma increased the mannose receptor CD206 in human macrophages suggesting a shift toward M2 phenotype . These pioneer findings suggest that various preparations containing platelets can control macrophage polarization, particularly the shift from M1 proinflammatory to M2 pro‐resolving phenotypes.…”

Section: Introductionmentioning

confidence: 88%

“…17 Moreover, leukocyte-depleted platelet-rich plasma increased the mannose receptor CD206 in human macrophages suggesting a shift toward M2 phenotype. 18 These pioneer findings suggest that various preparations containing platelets can control macrophage polarization, particularly the shift from M1 proinflammatory to M2 pro-resolving phenotypes. However, studies addressing PRF and macrophage polarization have not yet been performed.…”

Section: Introductionmentioning

confidence: 99%

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Platelet‐rich fibrin elicits an anti‐inflammatory response in macrophages in vitro

Nasirzade

Kargarpour

Hasannia

et al. 2019

Journal of Periodontology

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Background Platelet‐rich fibrin (PRF) serves as a reservoir of bioactive molecules to support wound healing and bone regeneration. The beneficial action of PRF might involve macrophage polarization from proinflammatory M1 toward pro‐resolving M2 phenotypes. This study aims to evaluate the effect of PRF on macrophage polarization. Methods Murine primary macrophages and RAW 264.7 cells were exposed to saliva and lipopolysaccharides (LPS) with and without PRF lysates obtained by repeated freeze‐thawing or the secretome of PRF membranes, termed PRF conditioned medium. The expression of the M1 marker genes interleukin 1β (IL1β) and interleukin 6 (IL6) along with the M2 markers arginase‐1 and chitinase‐like 3 (Chil3 or YM1) were evaluated by real time polymerase chain reaction. Immunoassay and immunofluorescence staining were performed for IL6 and p65 translocation, a subunit nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐kB), respectively. Results We report here that PRF lysates and PRF conditioned medium, the latter containing the secretome, greatly decreased the proinflammatory response of primary macrophages and RAW 264.7 cells as indicated by the expression of IL1β and IL6. The anti‐inflammatory activity of PRF lysates was further confirmed by IL6 immunoassay. Moreover, PRF lysates suppressed the translocation of p65 from the cytoplasm into the nucleus after incubation with saliva. In support of M2 polarization, PRF lysates and PRF conditioned medium enhanced the expression of arginase‐1 and YM1 in primary macrophages. Conclusion Our results indicate that PRF holds an anti‐inflammatory activity and shifts the macrophage polarization from an M1 toward an M2 phenotype.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Platelet‐rich fibrin elicits an anti‐inflammatory response in macrophages in vitro

Nasirzade

Kargarpour

Hasannia

et al. 2019

Journal of Periodontology

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“…However, when macrophages were challenged with pro-or anti-inflammatory factors (M1 and M2 medium, respectively), the secretion of both pro-(MIP1α and TNFα) and anti-inflammatory (IL1-ra) cytokines were modulated; PRPr dampened the production of MIP1α and the content released from our composite scaffolds allowed macrophages to secrete both MIP1α and TNFα to the same levels as 10% FBS, while increasing the level of IL1ra secretion. Indeed, anti-inflammatory effects of PRP have previously been reported in different applications (Zhang et al, 2013;Moussa et al, 2017;Abdul Ameer et al, 2018); although other groups reported stimulation of both M1 and M2 markers (Escobar et al, 2018). The study of macrophage polarization is an evolving field, with several cytokines and markers expressed by subpopulations that may go beyond the classical M1/M2 and pro-/anti-inflammatory phenotypes (Chávez-Galán et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Functionalising Collagen-Based Scaffolds With Platelet-Rich Plasma for Enhanced Skin Wound Healing Potential

Amaral

Zayed

Pascu

et al. 2019

Front. Bioeng. Biotechnol.

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Porous collagen-glycosaminoglycan (collagen-GAG) scaffolds have shown promising clinical results for wound healing; however, these scaffolds do not replace the dermal and epidermal layer simultaneously and rely on local endogenous signaling to direct healing. Functionalizing collagen-GAG scaffolds with signaling factors, and/or additional matrix molecules, could help overcome these challenges. An ideal candidate for this is platelet-rich plasma (PRP) as it is a natural reservoir of growth factors, can be activated to form a fibrin gel, and is available intraoperatively. We tested the factors released from PRP (PRPr) and found that at specific concentrations, PRPr enhanced cell proliferation and migration and induced angiogenesis to a greater extent than fetal bovine serum (FBS) controls. This motivated us to develop a strategy to successfully incorporate PRP homogeneously within the pores of the collagen-GAG scaffolds. The composite scaffold released key growth factors for wound healing (FGF, TGFβ) and vascularization (VEGF, PDGF) for up to 14 days. In addition, the composite scaffold had enhanced mechanical properties (when compared to PRP gel alone), while providing a continuous upper surface of extracellular matrix (ECM) for keratinocyte seeding. The levels of the factors released from the composite scaffold were sufficient to sustain proliferation of key cells involved in wound healing, including human endothelial cells, mesenchymal stromal cells, fibroblasts, and keratinocytes; even in the absence of FBS supplementation. In functional in vitro and in vivo vascularization assays, our composite scaffold demonstrated increased angiogenic and vascularization potential, which is known to lead to enhanced wound healing. Upon pro-inflammatory induction, macrophages released lower levels of the pro-inflammatory marker MIP-1α when treated with PRPr; and released higher levels of the anti-inflammatory marker IL1-ra upon both pro-and anti-inflammatory induction when treated with the composite scaffold. Finally, our composite scaffold supported a co-culture system of human fibroblasts and do Amaral et al. PRP Incorporation Into Collagen-Based Scaffold keratinocytes that resulted in an epidermal-like layer, with keratinocytes constrained to the surface of the scaffold; by contrast, keratinocytes were observed infiltrating the PRPfree scaffold. This novel composite scaffold has the potential for rapid translation to the clinic by isolating PRP from a patient intraoperatively and combining it with regulatory approved scaffolds to enhance wound repair.

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Platelet-Rich Plasma and Wound Healing

Xu,

Dong

2023

Platelet-Rich Plasma in Tissue Repair and Regeneration

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Pure Platelet-Rich Plasma and Supernatant of Calcium-Activated P-PRP Induce Different Phenotypes of Human Macrophages

Abstract: These platelet preparations do not have an equivalent biological effect over macrophages, which suggest that they may present different clinical regenerative potentials.

Cited by 29 publications

References 49 publications

Platelet‐rich fibrin elicits an anti‐inflammatory response in macrophages in vitro

Platelet‐rich fibrin elicits an anti‐inflammatory response in macrophages in vitro

Functionalising Collagen-Based Scaffolds With Platelet-Rich Plasma for Enhanced Skin Wound Healing Potential

Platelet-Rich Plasma and Wound Healing

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