Pure cultures for synthetic culture development: Next level municipal waste treatment for polyhydroxyalkanoates production

Khatami, Kasra; Pérez-Zabaleta, Mariel; Cetecioglu, Zeynep

doi:10.1016/j.jenvman.2021.114337

Cited by 14 publications

(8 citation statements)

References 48 publications

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“…For the efficient utilization of chemically pretreated LC biomass hydrolysates and PHA production, using individual culture has limitations. It was observed that the buildout of microbial co-culture of different species with diverse metabolic activities would be a viable solution for effective LC biomass to PHA conversion accompanied by desired co-polymers production [39][40][41].…”

Section: Pha Production Studiesmentioning

confidence: 99%

Developing Microbial Co-Culture System for Enhanced Polyhydroxyalkanoates (PHA) Production Using Acid Pretreated Lignocellulosic Biomass

et al. 2022

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In the growing polymer industry, the interest of researchers is captivated by bioplastics production with biodegradable and biocompatible properties. This study examines the polyhydroxyalkanoates (PHA) production performance of individual Lysinibacillus sp. RGS and Ralstonia eutropha ATCC 17699 and their co-culture by utilizing sugarcane bagasse (SCB) hydrolysates. Initially, acidic (H2SO4) and acidified sodium chlorite pretreatment was employed for the hydrolysis of SCB. The effects of chemical pretreatment on the SCB biomass assembly and its chemical constituents were studied by employing numerous analytical methods. Acidic pretreatment under optimal conditions showed effective delignification (60%) of the SCB biomass, leading to a maximum hydrolysis yield of 74.9 ± 1.65% and a saccharification yield of 569.0 ± 5.65 mg/g of SCB after enzymatic hydrolysis. The resulting SCB enzymatic hydrolysates were harnessed for PHA synthesis using individual microbial culture and their defined co-culture. Co-culture strategy was found to be effective in sugar assimilation, bacterial growth, and PHA production kinetic parameters relative to the individual strains. Furthermore, the effects of increasing acid pretreated SCB hydrolysates (20, 30, and 40 g/L) on cell density and PHA synthesis were studied. The effects of different cost-effective nutrient supplements and volatile fatty acids (VFAs) with acid pretreated SCB hydrolysates on cell growth and PHA production were studied. By employing optimal conditions and supplementation of corn steep liquor (CSL) and spent coffee waste extracted oil (SCGO), the co-culture produced maximum cell growth (DCW: 11.68 and 11.0 g/L), PHA accumulation (76% and 76%), and PHA titer (8.87 and 8.36 g/L), respectively. The findings collectively suggest that the development of a microbial co-culture strategy is a promising route for the efficient production of high-value bioplastics using different agricultural waste biomass.

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Section: Pha Production Studiesmentioning

confidence: 99%

Developing Microbial Co-Culture System for Enhanced Polyhydroxyalkanoates (PHA) Production Using Acid Pretreated Lignocellulosic Biomass

et al. 2022

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“…Alternatively, it is possible to apply an open mixed microbial culture (MMC) to produce low-cost PHAs. MMC enables the use of inexpensive feedstocks such as waste/wastewater under non-sterile conditions. − This approach involves anaerobic fermentation of waste/wastewater to produce volatile fatty acids (VFAs), followed by aerobic conversion of VFAs into PHAs. During the fermentation process in the MMC, VFAs with an odd number of carbons will produce the poly(3-hydroxybutyrate- co -3-hydroxyvalerate) (PHBV) copolymer with varying hydroxyvalerate (HV) ratios and those with an even number of carbons will yield poly(3-hydroxybutyrate) (PHB) .…”

Section: Introductionmentioning

confidence: 99%

Crotonic Acid Production by Pyrolysis and Vapor Fractionation of Mixed Microbial Culture-Based Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

Elhami

Hempenius

Schuur

2023

Ind. Eng. Chem. Res.

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Crotonic acid (CA) and trans-2-pentenoic acid (2-PA) can be obtained from renewable resources by pyrolysis of the bio-based poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymer. In this study, direct pyrolysis of the PHBV-enriched biomass into CA and 2-PA was studied under an inert atmosphere and N2 carrier gas flow, aiming at obtaining high acid yields from mixed microbial cultures (MMCs). The highest yields of 80 ± 2% for CA and 67 ± 1% for 2-PA were obtained at conditions of 240 °C, 1 h, and 0.15 L/min nitrogen flow rate, corresponding to a mean hot vapor residence time of 20 s. A similar acid yield was achieved when the pyrolysis was performed under reduced pressure (150 mbar) instead of using nitrogen gas. The combined pyrolysis of extracted PHBV at 220 °C and 90 min with vapor fractionation by distillation resulted in yields of 81 and 92% for CA and 2-PA, respectively.

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“…Given this, many recent studies still use single wild‐type bacteria for PHAs synthesis, in which Cupriavidus necator ( C. necator ) and Halomonas sp. are most widely adopted for their high growth rate and remarkable capacities of PHAs accumulation [10, 18–20]. As reported, both C. necator and Halomonas sp.…”

Section: Introductionmentioning

confidence: 99%

“…As reported, both C. necator and Halomonas sp. achieved PHAs contents of over 90% [10, 19]. However, the growth condition of Halomonas sp.…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis of poly(3‐hydroxybutyrate‐co‐3‐hydroxvalerate) from volatile fatty acids by Cupriavidus necator

Cai

Jin

et al. 2022

J Basic Microbiol

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A promising strategy to alleviate the plastic pollution from traditional petroleum‐based plastics is the application of biodegradable plastics, in which polyhydroxyalkanoates (PHAs) have received increasing interest owing to their considerable biodegradability. In the PHAs family, poly(3‐hydroxybutyrate‐co‐3‐hydroxvalerate) (PHBV) has better mechanical properties, which possesses broader application prospects. With this purpose, the present study adopted Cupriavidus necator to synthesize PHBV utilizing volatile fatty acids (VFAs) as sole carbon sources. Results showed that the concentration and composition of VFAs significantly influenced the production of PHAs. Especially, even carbon VFAs (acetate and butyrate) synthesized only poly(3‐hydroxybutyrate) (PHB), while the addition of odd carbon VFAs (propionate and valerate) resulted in PHBV production. The 3‐hydroxyvalerate (3HV) contents in PHBV were directly determined by the specific VFAs compositions, in which valerate was the preferred substrate for 3HV accumulation. After optimization by response surface methodology, the highest PHBV accumulation achieved 79.47% in dry cells, and the conversion efficiency of VFAs to PHBV reached 40%, with the PHBV production of 1.20 ± 0.05 g/L. This study revealed the metabolic rule of VFAs converting into PHAs by C. necator and figured out the optimal VFAs condition for PHBV accumulation, which provides a valuable reference for developing downstream strategies of PHBV production in industrial applications in future.

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Pure cultures for synthetic culture development: Next level municipal waste treatment for polyhydroxyalkanoates production

Cited by 14 publications

References 48 publications

Developing Microbial Co-Culture System for Enhanced Polyhydroxyalkanoates (PHA) Production Using Acid Pretreated Lignocellulosic Biomass

Developing Microbial Co-Culture System for Enhanced Polyhydroxyalkanoates (PHA) Production Using Acid Pretreated Lignocellulosic Biomass

Crotonic Acid Production by Pyrolysis and Vapor Fractionation of Mixed Microbial Culture-Based Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

Biosynthesis of poly(3‐hydroxybutyrate‐co‐3‐hydroxvalerate) from volatile fatty acids by Cupriavidus necator

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