Pur alpha and Sp8 as opposing regulators of neural gata2 expression

Penberthy, W. Todd; Zhao, Chengtian; Jessen, Jason R.; Yang, Zhongan; Bricaud, Olivier; Collazo, Andrés; Meng, Anming; Lin, Shuo

doi:10.1016/j.ydbio.2004.08.007

Cited by 25 publications

(29 citation statements)

References 45 publications

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“…2K, L; 5I-L). This is in agreement with those zebrafish studies that confine gata2 to hematopoietic and neuronal lineages (Meng et al, '97;Amatruda and Zon, '99;Penberthy et al, 2004).…”

Section: Hematopoietic Developmentsupporting

confidence: 92%

“…Some workers assign gata2 to a multiple role in hematopoiesis, angiogenesis and neurogenesis (Detrich et al, '95;Brown et al, 2000), whereas other studies consider it only as a marker of hematopoietic and neuronal development (Meng et al, '97;Amatruda and Zon, '99;Penberthy et al, 2004). The present ISH results tend to support this second view even though some of the findings may appear at first sight difficult to interpret.…”

Section: Hematopoietic Developmentsupporting

confidence: 45%

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Patterns of angiogenic and hematopoietic gene expression during brown trout embryogenesis

et al. 2008

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In this article, whole mount in situ hybridization is used to examine early blood vessel and blood cell development in the embryos of the brown trout Salmo trutta lacustris. cDNAs encoding for the angiogenic markers fli1 and flk1, and for the hematopoietic markers gata1 and gata2, were identified from an expressed sequence tag library of rainbow trout. Results show that fli1, flk1 and gata2 are activated in bilateral bands of the lateral trunk mesoderm before the onset of somitogenesis, shortly followed by gata1. These bands then converge toward the ventral midline to form the intermediate cell mass (ICM) (anterior ICM). Subsequent axial vasculogenesis and initial blood cell formation involve a clear spatial separation of fli1 and gata gene expression. Fli1 staining is most intense within the axial vessel (dorsal aorta, posterior cardinal vein) forming and lateral ICM cells, whereas binding of gata1 and gata2 probes becomes confined to the central portion of ICM cells beneath the dorsal aorta. This is followed by a first wave of angiogenesis, indicated by expression of fli1 and flk1. This gives rise to the intersegmental, dorsal longitudinal anastomotic and intestinal vessels. Further angiogenesis and hematopoiesis are activated in the "posterior ICM" of the tail. Here, the absence of gata1 indicates that hematopoiesis in this tissue generates myeloid rather than erythroid cells. The results supplement and validate previous, now historical morphological work in salmonids, thus aiding the elucidation of a comprehensive general scheme of angiogenic and hematopoietic development in the teleost embryo.

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“…2K, L; 5I-L). This is in agreement with those zebrafish studies that confine gata2 to hematopoietic and neuronal lineages (Meng et al, '97;Amatruda and Zon, '99;Penberthy et al, 2004).…”

Section: Hematopoietic Developmentsupporting

confidence: 92%

Section: Hematopoietic Developmentsupporting

confidence: 45%

Patterns of angiogenic and hematopoietic gene expression during brown trout embryogenesis

et al. 2008

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“…It will be interesting to determine whether there are shared targets of sptf-3 and Sp8. In support of this, disruption of Sp8 in zebrafish embryos has been shown to result in decreased expression of gata2, a GATA transcription factor, in the neuroepithelium (Penberthy et al 2004). The role of gata2 during neural tube closure is not clear because mutations in mice result in early embryonic lethality; however, evidence suggests that gata2 may affect early neuronal differentiation (Nardelli et al 1999;Craven et al 2004).…”

Section: Discussionmentioning

confidence: 98%

Identifying Regulators of Morphogenesis Common to Vertebrate Neural Tube Closure andCaenorhabditis elegansGastrulation

Sullivan-Brown

Tandon

et al. 2015

Genetics

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Neural tube defects including spina bifida are common and severe congenital disorders. In mice, mutations in more than 200 genes can result in neural tube defects. We hypothesized that this large gene set might include genes whose homologs contribute to morphogenesis in diverse animals. To test this hypothesis, we screened a set of Caenorhabditis elegans homologs for roles in gastrulation, a topologically similar process to vertebrate neural tube closure. Both C. elegans gastrulation and vertebrate neural tube closure involve the internalization of surface cells, requiring tissue-specific gene regulation, actomyosin-driven apical constriction, and establishment and maintenance of adhesions between specific cells. Our screen identified several neural tube defect gene homologs that are required for gastrulation in C. elegans, including the transcription factor sptf-3. Disruption of sptf-3 in C. elegans reduced the expression of early endodermally expressed genes as well as genes expressed in other early cell lineages, establishing sptf-3 as a key contributor to multiple well-studied C. elegans cell fate specification pathways. We also identified members of the actin regulatory WAVE complex (wve-1, gex-2, gex-3, abi-1, and nuo-3a). Disruption of WAVE complex members reduced the narrowing of endodermal cells' apical surfaces. Although WAVE complex members are expressed broadly in C. elegans, we found that expression of a vertebrate WAVE complex member, nckap1, is enriched in the developing neural tube of Xenopus. We show that nckap1 contributes to neural tube closure in Xenopus. This work identifies in vivo roles for homologs of mammalian neural tube defect genes in two manipulable genetic model systems.

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“…Sp8 and Sp9 are expressed during zebrafish somitogenesis within the developing central nervous system and fin buds. Elimination of Sp9 expression in early embryos results in major defects in neurogenesis and brain segmentation, whereas loss of Sp8 and Sp9 expression in 2-day-old embryos is correlated with defects in fin outgrowth (49,50). As for sp5 and sp5l, Sp8 and Sp9 are regulated by Wnt/ ␤-catenin and fibroblast growth factor signals released by the mesenchyme to induce fin development (49).…”

Section: Discussionmentioning

confidence: 99%

Sp2 Is a Maternally Inherited Transcription Factor Required for Embryonic Development

Xie

Yin

Nichols

et al. 2010

Journal of Biological Chemistry

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The Sp family of transcription factors is required for the expression of cell cycle-and developmentally regulated genes, and the deregulated expression of a handful of family members is associated with human tumorigenesis. Sp2 is a relatively poorly characterized member of the Sp family that, although widely expressed, exhibits little or no DNA binding or transcriptional activity in human and mouse cell lines. To begin to address the role(s) played by Sp2 in early metazoan development we have cloned and characterized Sp2 from zebrafish (Danio rerio). We report that 1) the intron/exon organization and amino acid sequence of zebrafish Sp2 is closely conserved with its mammalian orthologues, 2) zebrafish Sp2 weakly stimulates an Sp-dependent promoter in vitro and associates with the nuclear matrix in a DNA-independent fashion, 3) zebrafish Sp2 is inherited as a maternal transcript, is transcribed in zebrafish embryos and adult tissues, and is required for completion of gastrulation, and 4) zebrafish lines carrying transgenes regulated by the Sp2 promoter recapitulate patterns of endogenous Sp2 expression.Sp/XKLF proteins are sequence-specific DNA-binding proteins that share a highly conserved, carboxyl-terminal DNA binding domain featuring three "zinc fingers" of the Cys 2 -His 2 class (1-3). Four conserved nucleotides within each zinc finger specify the interaction of the DNA binding domain with a nonameric GC-rich sequence that is often located proximal to sites of transcriptional initiation. Sp proteins comprise one subfamily of Sp/XKLF proteins for which nine members (Sp1-Sp9) have been described in mammals. Each family member carries an amino-terminal trans-activation domain that is comprised of three subdomains, termed A, B, and C (4). The A and B subdomains feature alternating regions enriched in glutamine or serine/threonine residues, whereas the C subdomain carries an abundance of charged amino acids (5, 6). Each subdomain is sufficient to drive transcription when tethered individually to a DNA binding domain, albeit to levels that are less significant than elicited when multiple subdomains are examined together. trans-Activation of target genes by Sp proteins is dependent on post-translational modifications as well as their physical interaction with each other, additional sequence-specific DNA-binding proteins, and components of the basal transcription complex.The biochemical and functional properties of Sp1, Sp3, and Sp4 have been characterized most thoroughly, and each has been shown to regulate the expression of a constellation of genes involved in fundamental biological processes, including cell cycle control, differentiation, development, and oncogenesis (1, 7-9). Sp1, Sp2, and Sp3 are expressed in many, if not all, mammalian tissues, whereas the expression of Sp4 -9 is considerably more restricted. Sp-related proteins have been identified in a wide variety of species, including zebrafish and invertebrates, and mechanisms governing Sp-dependent transcription are evolutionarily conserved. For example,...

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Pur alpha and Sp8 as opposing regulators of neural gata2 expression

Cited by 25 publications

References 45 publications

Patterns of angiogenic and hematopoietic gene expression during brown trout embryogenesis

Patterns of angiogenic and hematopoietic gene expression during brown trout embryogenesis

Identifying Regulators of Morphogenesis Common to Vertebrate Neural Tube Closure andCaenorhabditis elegansGastrulation

Sp2 Is a Maternally Inherited Transcription Factor Required for Embryonic Development

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