Punica granatum Peel Extract Toxicity in Mice

Abstract

Background: Pomegranate (Punica granatum) is a significant source of bioactive compounds. However, its toxicity is not intensively studied. Objectives: The current study investigated the safety and tolerability of pomegranate peel extract (PPE) in BALB/c mice. Materials and Methods: A total of 25 female BALB/c mice were randomly grouped. Each experimental group consisted of five animals. Repeated doses including 0.5, 1.9 and 7.5 mg/kg body weight of PPE were gavaged to BALB/c mice, for 22 days and the single intra-dermal injection (224 mg/kg) was done in one dose. The control group administrated with distilled water was also included. In addition, intra dermal injection for skin allergy testing was also performed. Blood was collected to evaluate glucose, cholesterol, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as indicators of liver toxicity. Macroscopic and histopathological evaluation of tongue, trachea, and larynx tissues were also performed on 22 days post administration. Results: Toxicological potential of PPE studies revealed no toxic effects, clinical signs, histopathological effect in epithelial cells layer of tongue, larynx and trachea, behavioral alterations and adverse effects or mortality in BALB/c mice. Repeated administrations did not alter or cause local irritation of the oral mucosa. Skin allergy test was negative in the last group. Conclusions: The current study showed that PPE had no toxicity and its use is suggested with potential applications against diseases.

Protective effect of pomegranate (Punica granatum) extract against diabetic changes in adult male rat liver: Histological study

“…Bassiri Jahromi et al [18] found that administration of PEE (repeated doses of 0.5, 1.9, and 7.5 mg/kg body weight) to mice did not produce any irritation to oral mucosa or respiratory passage. Further, single intradermal injection (224 mg//kg) of the extract was found to be safe without any manifestation of skin allergy.…”

Bactericidal, protistocidal, nematodicidal properties and chemical composition of ethanol extract of Punica granatum peel

“…The effect of extracts from P. granatum on S. papillosus larvae is currently unstudied. Jahromi et al (2015) studied the safety of the extract of pomegranade peel for mice. Doses of 0.5, 1.9 and 7.5 mg/kg of body weight introduced over 22 days displayed no toxic effect according to clinical signs and histopreparations.…”

Pomegranate Peel Phytochemistry, Pharmacological Properties, Methods of Extraction, and Its Application: A Comprehensive Review

“…None of the animals developed any hematological or biochemical abnormalities, and all mice survived until the end of the study. Additionally, no allergic reaction was shown after 24, 48, and 72 h. 122 The study found that pomegranate peel, a natural extract with excellent anti-inflammatory and antioxidant activities, was potent against autoimmune hepatitis (AIH) in a ConAinduced AIH mouse model. The results showed that PPE had substantial anti-inflammatory and protective effects on the occurrence and development of AIH.…”