Pumpkin seeds (Cucurbita pepo subsp. ovifera) decoction promotes Trichinella spiralis expulsion during intestinal phase via “Weep and Sweep” mechanism
Aml S. Saleh,
Samah A. El-Newary,
Walaa A. Mohamed
et al.
Abstract:Trichinosis is a zoonotic disease of communal health concern as it instigated human outbreaks in several countries. Besides, the development of resistance, traditional therapy has numerous antagonistic effects. Thereby, finding efficient natural alternatives is required. In comparison to albendazole, this study evaluated the impact of pumpkin decoction on Trichinella spiralis in experimentally infected mice. The anthelmintic action of pumpkin decoction (500 mg/kg) was determined using T. spiralis infected mice… Show more
Purpose: This study aims to explore the anthelmintic potential of the ethanolic extract derived from the discarded stem of Schumannianthus dichotomus on the cestode Raillietina spp. Additionally, phytochemical screening of the extracts seeks to elucidate the presence of bioactive compounds responsible for the observed anthelmintic activity.
Methods: Raillietina spp., the model parasite, was collected from the intestine of freshly slaughtered fowl and treated with different doses of ethanolic extract and fractions of Schumannianthus dichotomus for motility assays to determine the most efficacious dose. Changes in the ultrastructure of the worms were investigated through TEM and SEM. Qualitative and quantitative analysis of phytochemicals in the crude extract as well as GCMS analysis of the ethyl acetate fraction were also done.
Results: The worms showed dose dependent reduction in motility and survival. The most efficacious dose and fraction were determined to be 20 mg/ml and ethyl acetate fraction respectively. Changes in tegument and internal structures were evidenced by SEM and TEM observations. The crude extract was found rich in alkaloids, flavonoids and terpenoids. GCMS analysis of the ethyl acetate fraction identified five major compounds out of which Phthalic acid, di(2-propylpentyl) ester may be the major bioactive component responsible for the anthelmintic activity.
Conclusion: Our study firmly establishes the anthelmintic potential of the waste part of Schumannianthus dichotomusand prospects its valorisation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.