Pumping iron to keep fit: modulation of siderophore secretion helps efficient aromatic utilization in Pseudomonas putida KT2440

Joshi, Hiren; Dave, Rachna; Venugopalan, V.P.

doi:10.1099/mic.0.079277-0

Cited by 15 publications

(13 citation statements)

References 38 publications

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“…Some species of fungi such as Fusarium sp. and bacteria such as P. aeruginosa were able to produce element-specific ligands (siderophores) that are able to change pH and enhance the chelation of some elements such as Uranium (U 6+ ) and Thorium (Th 4+ ) (Joshi et al 2014).…”

Section: Environmental Applicationsmentioning

confidence: 99%

“…Stimulated siderophore secretion might be a key factor in successful integration and proliferation of this organism as a bioaugmentation agent for aromatic degradation. It not only facilitates efficient aro-matic utilisation but also provides better opportunities for iron assimilation amongst diverse microbial communities, thereby ensuring better survival and proliferation (Joshi et al 2014).…”

Section: Environmental Applicationsmentioning

confidence: 99%

See 1 more Smart Citation

Siderophore: Structural And Functional Characterisation – A Comprehensive Review

Sah¹,

Singh²

2015

Agriculture (Polnohospodárstvo)

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Plants and microbes have enormous importance in our daily life. Iron is said to be the fourth most abundant element in the earth's crust from soil, still many plants face problem in uptaking iron because it is found in insoluble form, which severely restricts the bioavailability of this metal. In response to this, microorganisms present in soil such as Pseudomonas sp., Enterobacter genera, Bacillus and Rhodococcus produce special iron carriers or iron-binding compounds called as ‘siderphores’ or ‘siderochromes’. This paper is an attempt to review the importance of siderphores in enhancing plants’ iron utilisation strategies, the mode of transport of siderophores along with iron across the memberane and depending on the difference in their chemical structure, functional moiety and their source of isolation of four different types of siderophore (hydroxamates, catecholates, carboxylates and siderophore with mixed ligand). Siderophore and their derivative have large application in agriculture as to increase soil fertility and as biocontrol for fungal pathogen. This review unlike other reviews includes (1) types of siderophore, (2) the structural difference amongst them, (3) siderophore biosynthesis, (4) transport mechanism, (5) the genetics of siderophore and (6) their efficacy in human life.

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Section: Environmental Applicationsmentioning

confidence: 99%

Section: Environmental Applicationsmentioning

confidence: 99%

Siderophore: Structural And Functional Characterisation – A Comprehensive Review

Sah¹,

Singh²

2015

Agriculture (Polnohospodárstvo)

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“…Siderophore has been demonstrated to be an important factor influencing biofilm development, especially under ironlimiting conditions (Visca et al 2007). It has also been shown to play an important role in biodegradation of aromatic compounds by P. putida (Joshi et al 2014). An increased secretion of siderophore was observed when cells were exposed to benzyl alcohol, which would facilitate degradation process and boost bacterial growth because oxygenases for degradation are iron-dependent (Joshi et al 2014).…”

Section: Biosynthesis Of Extracellular Polysaccharidesmentioning

confidence: 99%

“…It has also been shown to play an important role in biodegradation of aromatic compounds by P. putida (Joshi et al 2014). An increased secretion of siderophore was observed when cells were exposed to benzyl alcohol, which would facilitate degradation process and boost bacterial growth because oxygenases for degradation are iron-dependent (Joshi et al 2014). The capability of producing and uptaking siderophores by C. testosteroni strain I2 renders this strain more competitive, especially under ironlimiting conditions, and this capability may also benefit its catabolic activities.…”

Section: Biosynthesis Of Extracellular Polysaccharidesmentioning

confidence: 99%

Comparative genome analysis reveals genetic adaptation to versatile environmental conditions and importance of biofilm lifestyle in Comamonas testosteroni

Arumugam

Tay

et al. 2015

Appl Microbiol Biotechnol

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Comamonas testosteroni is an important environmental bacterium capable of degrading a variety of toxic aromatic pollutants and has been demonstrated to be a promising biocatalyst for environmental decontamination. This organism is often found to be among the primary surface colonizers in various natural and engineered ecosystems, suggesting an extraordinary capability of this organism in environmental adaptation and biofilm formation. The goal of this study was to gain genetic insights into the adaption of C. testosteroni to versatile environments and the importance of a biofilm lifestyle. Specifically, a draft genome of C. testosteroni I2 was obtained. The draft genome is 5,778,710 bp in length and comprises 110 contigs. The average G+C content was 61.88 %. A total of 5365 genes with 5263 protein-coding genes were predicted, whereas 4324 (80.60 % of total genes) protein-encoding genes were associated with predicted functions. The catabolic genes responsible for biodegradation of steroid and other aromatic compounds on draft genome were identified. Plasmid pI2 was found to encode a complete pathway for aniline degradation and a partial catabolic pathway for chloroaniline. This organism was found to be equipped with a sophisticated signaling system which helps it find ideal niches and switch between planktonic and biofilm lifestyles. A large number of putative multi-drug-resistant genes coding for abundant outer membrane transporters, chaperones, and heat shock proteins for the protection of cellular function were identified in the genome of strain I2. In addition, the genome of strain I2 was predicted to encode several proteins involved in producing, secreting, and uptaking siderophores under iron-limiting conditions. The genome of strain I2 contains a number of genes responsible for the synthesis and secretion of exopolysaccharides, an extracellular component essential for biofilm formation. Overall, our results reveal the genomic features underlying the adaption of C. testosteroni to versatile environments and highlighting the importance of its biofilm lifestyle.

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“…Pyoverdine-mediated iron uptake is essential for surface motility of P. putida KT2440 (25) and has been described as a key factor involved in growth inhibition of the plant pathogen Xanthomonas fragariae by KT2440 (26). Increased siderophore production has been reported to take place during growth on the aromatic compound benzyl alcohol as a carbon and energy source (27). Besides an increase in pyoverdine production, iron limitation also results in changes in the metabolic flux and increased gluconate secretion during growth of P. putida KT2440 on glucose (28).…”

mentioning

confidence: 99%

Arginine Biosynthesis Modulates Pyoverdine Production and Release in Pseudomonas putida as Part of the Mechanism of Adaptation to Oxidative Stress

et al. 2019

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Iron is essential for most life forms. Under iron-limiting conditions, many bacteria produce and release siderophores—molecules with high affinity for iron—which are then transported into the cell in their iron-bound form, allowing incorporation of the metal into a wide range of cellular processes. However, free iron can also be a source of reactive oxygen species that cause DNA, protein, and lipid damage. Not surprisingly, iron capture is finely regulated and linked to oxidative-stress responses. Here, we provide evidence indicating that in the plant-beneficial bacterium Pseudomonas putida KT2440, the amino acid l-arginine is a metabolic connector between iron capture and oxidative stress. Mutants defective in arginine biosynthesis show reduced production and release of the siderophore pyoverdine and altered expression of certain pyoverdine-related genes, resulting in higher sensitivity to iron limitation. Although the amino acid is not part of the siderophore side chain, addition of exogenous l-arginine restores pyoverdine release in the mutants, and increased pyoverdine production is observed in the presence of polyamines (agmatine and spermidine), of which arginine is a precursor. Spermidine also has a protective role against hydrogen peroxide in P. putida, whereas defects in arginine and pyoverdine synthesis result in increased production of reactive oxygen species. IMPORTANCE The results of this study show a previously unidentified connection between arginine metabolism, siderophore turnover, and oxidative stress in Pseudomonas putida. Although the precise molecular mechanisms involved have yet to be characterized in full detail, our data are consistent with a model in which arginine biosynthesis and the derived pathway leading to polyamine production function as a homeostasis mechanism that helps maintain the balance between iron uptake and oxidative-stress response systems.

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Pumping iron to keep fit: modulation of siderophore secretion helps efficient aromatic utilization in Pseudomonas putida KT2440

Cited by 15 publications

References 38 publications

Siderophore: Structural And Functional Characterisation – A Comprehensive Review

Siderophore: Structural And Functional Characterisation – A Comprehensive Review

Comparative genome analysis reveals genetic adaptation to versatile environmental conditions and importance of biofilm lifestyle in Comamonas testosteroni

Arginine Biosynthesis Modulates Pyoverdine Production and Release in Pseudomonas putida as Part of the Mechanism of Adaptation to Oxidative Stress

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