2008
DOI: 10.1007/s00427-007-0200-1
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Pumilio genes from the Platyhelminthes

Abstract: Pumilio proteins are proposed to have a conserved primordial function in the maintenance of proliferation in stem cells through post-transcriptional regulation. In this work, a search for pumilio homology domain (PUM-HD) sequences of pumilio genes from several Platyhelminthes species was performed, including representatives form Cestoda, Trematoda and Tricladida. Only one PUM-HD sequence was found in each triclad species; however, two PUM-HD homologues were found in all the parasitic species. These sequences f… Show more

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Cited by 6 publications
(5 citation statements)
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“…Unlike the Larvae, however, DE GOI in the Scolex-Neck also included five components of TGF-β/BMP signalling, including tgfb and bmp2 -like genes, an ortholog of Hedgehog, the primary ligand of the Hedgehog signalling pathway, and a tef - 5 -like transcript associated with Hippo signalling [ 90 , 91 ]. One of three paralogs of the stem cell regulator Pumilio [ 92 , 93 ] ( pum1 ) is up-regulated relative to the End sample (but not to the Mid), whereas the remaining paralogs, pum2 and pum3 , are independently up-regulated in the Mid and End samples, respectively (Table 2 ).…”
Section: Resultsmentioning
confidence: 99%
“…Unlike the Larvae, however, DE GOI in the Scolex-Neck also included five components of TGF-β/BMP signalling, including tgfb and bmp2 -like genes, an ortholog of Hedgehog, the primary ligand of the Hedgehog signalling pathway, and a tef - 5 -like transcript associated with Hippo signalling [ 90 , 91 ]. One of three paralogs of the stem cell regulator Pumilio [ 92 , 93 ] ( pum1 ) is up-regulated relative to the End sample (but not to the Mid), whereas the remaining paralogs, pum2 and pum3 , are independently up-regulated in the Mid and End samples, respectively (Table 2 ).…”
Section: Resultsmentioning
confidence: 99%
“…The intron neutral substitution rate was estimated from the average of K2P+G estimates of both genes using the formula r = s/2t, where r is the substitution rate, s the estimated substitutions per site, and t the time of divergence (set as 3 Myr for E. vogeli and E. oligarthrus ; Knapp et al 2011 ). For E. multilocularis , E. granulosus , and T. solium , divergence values were also estimated for other well-characterized genes ( emmpk2 , emsmadC , emmpk1 , emraf , emegfr , and emir1 from E. multilocularis [ Spiliotis et al 2003 , 2005 , 2006 ; Gelmedin et al 2008 ; Zavala-Gongora et al 2008 ; Hemer et al 2014 ], egpum1 from E. granulosus [ Koziol et al 2008 ], and their orthologs retrieved from the other species). When comparing T. solium and E. multilocularis , only the shortest introns showing trustworthy alignments were included, and therefore the divergence values between both species are conservative.…”
Section: Methodsmentioning
confidence: 99%
“…Two previously isolated M. corti pumilio genes [ McPum1 and McPum2 (Koziol et al ., 2008)], as well as the vasa homologue McpL10 were evaluated as putative markers of germinative cells in M. corti . First, the levels of mRNA expression for these marker genes were evaluated by quantitative reverse transcription polymerase chain reaction during strobilar development.…”
Section: Resultsmentioning
confidence: 99%
“…Digoxigenin-labelled probes were synthesized by in vitro transcription with SP6 polymerase (New England Biolabs), using the DIG RNA labelling mix (Roche) as described by the manufacturer. A fragment of the target genes was amplified using degenerate primers [FwVasa: 5´-ATGGCNTGYGCNCARACNGGN-3á nd RvVasa: 5´-NCCCATRTCNARCATNCKRTC-3´for McpL10 gene, and primers described in Koziol et al (2008) for pumilio genes] and cloned in pGEM-T Easy (Promega). The McpL10 gene was cloned using the primers indicated above, prior to the sequencing and publication of the M. corti genome (Coghlan et al, 2019).…”
Section: Probe Labellingmentioning
confidence: 99%