Pumilicin 4, A Novel Bacteriocin with Anti-MRSA and Anti-VRE Activity Produced by Newly Isolated Bacteria Bacillus pumilus Strain WAPB4

Aunpad, Ratchaneewan; Na-Bangchang, K.

doi:10.1007/s00284-006-0632-2

Cited by 91 publications

(56 citation statements)

References 27 publications

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“…The antibacterial activity was reduced to 25% and 50% at pH 2 and 3, respectively, and was severely altered at pH higher than 10 ( Table 2). The heat-stable property was also observed in other antimicrobial metabolites produced by Bacillus species [14,15,38,39]. The stability of this antibacterial activity over a wide range of pH and after heat treatment might be useful in several industrial applications.…”

Section: Antimicrobial Activity Spectrummentioning

confidence: 82%

“…Only few antimicrobial compounds produced by the genus Bacillus exhibit inhibitory activity against methicillin-resistant Staphylococcus species [15]. The antimicrobial activity against methicillin-resistant S. aureus species has only been described for Bacillus subtilis producing macrolactin antibiotic [3] and Bacillus pumilis strain producing pumilicin 4 [15].…”

Section: Introductionmentioning

confidence: 99%

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Production of Anti-Methicillin-Resistant Staphylococcus Activity from Bacillus subtilis sp. Strain B38 Newly Isolated from Soil

Tabbene

Slimene

Bouabdallah

et al. 2008

Appl Biochem Biotechnol

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B38 bacterial strain, isolated from Tunisian soil showed a strong antimicrobial activity. Based on biochemical characterization and 16S rDNA sequence analysis, B38 strain was identified as Bacillus subtilis. Cell culture supernatant showed antibacterial activity against clinical isolates of methicillin-resistant Staphylococcus species and several Gram-positive and Gram-negative bacteria. Antifungal activity against phytopathogenic fungi was also observed. Antibacterial activity production started at early exponential growth phase, and maximum activity was reached at the stationary phase. This antibacterial activity was neither affected by proteases, lipase, and organic solvents, nor by surfactants. It was stable over a wide pH range and still active after autoclaving at 121 degrees C during 20 min. Thin layer chromatography followed by bioautography assay allowed the detection of four active spots with R(f) values of 0.30, 0.47, 0.70, and 0.82. The single spot with R (f) 0.30 showed antifungal activity, whereas the spots with R(f) values of 0.47, 0.70, and 0.82 exhibited antibacterial activity.

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Section: Antimicrobial Activity Spectrummentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Production of Anti-Methicillin-Resistant Staphylococcus Activity from Bacillus subtilis sp. Strain B38 Newly Isolated from Soil

Tabbene

Slimene

Bouabdallah

et al. 2008

Appl Biochem Biotechnol

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“…This bacterium is highly resistant to extreme environmental conditions such as low or no nutrient availability, desiccation, irradiation, H 2 O 2 and chemical disinfections (19). The ecological role of B. pumilus is emphasized by the fact that they do produce compounds antagonist to fungal and bacterial pathogens (4,6). Thus, B. pumilus is of considerable research interest to understand its physiological diversity, genetic relatedness with other Bacillus spp.…”

Section: Introductionmentioning

confidence: 99%

Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India

Parvathi¹,

Krishna²,

Jose³

et al. 2009

Braz. J. Microbiol.

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Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase activity and uniformly sensitive to antibiotics tested in this study. One strain of B. pumilus harboured cereulide synthetase gene cesB of B. cereus which was indistinguishable from rest of the isolates biochemically and by AP-PCR. This study reports, for the first time, the presence of the emetic toxin gene cesB in B. pumilus.

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“…Some B. pumilus strains show fungicidal activity and have been used as biological control agents against phytopathogenic fungi (9,42). Other B. pumilus strains have been reported to be plant growth-promoting rhizobacteria (25,36,57,77), while some others showed potent antibacterial activity (6). Still others have been used as probiotics (15,20,28).…”

Section: Discussionmentioning

confidence: 99%

Selection of a Bacillus pumilus Strain Highly Active against Ceratitis capitata (Wiedemann) Larvae

Molina

Caña-Roca

Osuna

et al. 2010

Appl Environ Microbiol

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Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), the Mediterranean fruit fly (medfly), is one of the most important fruit pests worldwide. The medfly is a polyphagous species that causes losses in many crops, which leads to huge economic losses. Entomopathogenic bacteria belonging to the genus Bacillus have been proven to be safe, environmentally friendly, and cost-effective tools to control pest populations. As no control method for C. capitata based on these bacteria has been developed, isolation of novel strains is needed. Here, we report the isolation of 115 bacterial strains and the results of toxicity screening with adults and larvae of C. capitata. As a result of this analysis, we obtained a novel Bacillus pumilus strain, strain 15.1, that is highly toxic to C. capitata larvae. The toxicity of this strain for C. capitata was related to the sporulation process and was observed only when cultures were incubated at low temperatures before they were used in a bioassay. The mortality rate for C. capitata larvae ranged from 68 to 94% depending on the conditions under which the culture was kept before the bioassay. Toxicity was proven to be a special characteristic of the newly isolated strain, since other B. pumilus strains did not have a toxic effect on C. capitata larvae. The results of the present study suggest that B. pumilus 15.1 could be considered a strong candidate for developing strategies for biological control of C. capitata.

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Pumilicin 4, A Novel Bacteriocin with Anti-MRSA and Anti-VRE Activity Produced by Newly Isolated Bacteria Bacillus pumilus Strain WAPB4

Cited by 91 publications

References 27 publications

Production of Anti-Methicillin-Resistant Staphylococcus Activity from Bacillus subtilis sp. Strain B38 Newly Isolated from Soil

Production of Anti-Methicillin-Resistant Staphylococcus Activity from Bacillus subtilis sp. Strain B38 Newly Isolated from Soil

Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India

Selection of a Bacillus pumilus Strain Highly Active against Ceratitis capitata (Wiedemann) Larvae

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