Pumilacidins from the Octocoral-Associated Bacillus sp. DT001 Display Anti-Proliferative Effects in Plasmodium falciparum

Abstract

Chemical examination of the octocoral-associated Bacillus species (sp.) DT001 led to the isolation of pumilacidins A (1) and C (2). We investigated the effect of these compounds on the viability of Plasmodium falciparum and the mechanism of pumilacidin-induced death. The use of inhibitors of protein kinase C (PKC) and phosphoinositide 3-kinase (PI3K) was able to prevent the effects of pumilacidins A and C. The results indicated also that pumilacidins inhibit parasite growth via mitochondrial dysfunction and decreased cytosolic Ca2+.

Pumilacidins A-E from Sediment-Derived Bacterium Bacillus sp. 4040 an Their Antimicrobial Activity Evaluation

“…for C 54 H 95 N 7 O 13 Na, 1072.6886). From these data, and comparison of NMR data (in DMSO-d 6 ) with those reported (in CD 3 OD), 20 compound 2 was identified as pumilacidin A.…”

“…16 Recently it was demonstrated that pumilacidins A and C inhibit the growth of malarial parasites in vitro. 20 In this work, the antimicrobial activity of all isolated compounds was evaluated against Pseudomonas aeruginosa, Escherichia coli, methicillin resistant Staphylococcus aureus, Bacillus subtilis and Candida albicans, but none of them showed antimicrobial activity at the maximum concentration tested (1000 μg mL -1 ).…”

Trends of antimalarial marine natural products: progresses, challenges and opportunities

“…The results also revealed that pumilacidins inhibited the growth of the parasite by mitochondrial dysfunction and decreased cytosolic Ca 2+ . 155,156 Companeramides A (323) and B (324) from a Panamanian cyanobacterium exhibited remarkable antiplasmodial activities (IC 50 ¼ 0.22-1.1 mM) against three strains of P. falciparum (D6, Dd2 and 7G8) without overt cytotoxicity to four human cancer cell lines at the doses tested (chloroquine, IC 50 ¼ 5 Â 10 À3 -0.08 mM). 157 Using a bioassay-guided and spectroscopic strategy, active dudawalamides A-D (325-328) with IC 50 values ranging from 3.5 to 10 mM were isolated from the collection of cyanobacterium Moorea producens.…”

Semisynthesis, Antiplasmodial Activity, and Mechanism of Action Studies of Isocoumarin Derivatives

“…Vero epithelial cells (African monkey kidney epithelial cells) were seeded in 96-well plates using RPMI-1640 medium (Sigma-Aldrich, USA) supplemented with 10% fetal bovine serum (Gibco, Invitrogen, Carlsbad, CA, USA) and 1% penicillin/streptomycin (stock mix at 10 000 units penicillin and 10 mg of streptomycin per mL in 0.9% NaCl). The assays were based on those described in Torres, Coronado, et al 25 The IC 50 was calculated using the Data Analysis Wizard complement of Excel 2000 (Microsoft, Seattle, WA, USA).…”