Pumice particle interface: a case study for immunoglobulin G purification

Alacabey, İhsan; Acet, Ömür; Önal, Burcu; Dikici, Emrah; Karakoç, Veyis; Gürbüz, Fatma; Alkan, Hüseyin; Odabaşı, Mehmet

doi:10.1007/s00289-020-03392-0

Cited by 15 publications

(6 citation statements)

References 42 publications

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“…Correlation between the equilibrium data and the Langmuir-1 isotherm model was found to be high (R 2 ) (Table 3). The Langmuir model is based on the assumption of homogeneous adsorption energies to the surface and the absence of adsorbate transition in the surface plane [40,[43][44][45][46][47][48][49][50].…”

Section: Isotherm Studiesmentioning

confidence: 99%

Antibiotic Removal from the Aquatic Environment with Activated Carbon Produced from Pumpkin Seeds

Alacabey

2022

Molecules

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Antibiotics are among the most critical environmental pollutant drug groups. Adsorption is one of the methods used to eliminate these pollutants. In this study, activated carbon was produced from pumpkin seed shells and subsequently modified with KOH. The adsorbent obtained through this procedure was used to remove ciprofloxacin from aqueous systems. Fourier Transform-Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), elemental, X-ray Photoelectron Spectroscopy (XPS), Brunauer–Emmett–Teller (BET) and Zeta analyses were used to characterize the adsorbent. The surface area, in particular, was found to be a very remarkable value of 2730 m2/g. The conditions of the adsorption experiments were optimized based on interaction time, adsorbent amount, pH and temperature. Over 99% success was achieved in removal operations carried out under the most optimal conditions, with an absorption capacity of 884.9 mg·g−1. In addition, the Langmuir isotherm was determined to be the most suitable model for the adsorption interaction.

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Section: Isotherm Studiesmentioning

confidence: 99%

Antibiotic Removal from the Aquatic Environment with Activated Carbon Produced from Pumpkin Seeds

Alacabey

2022

Molecules

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“…The equilibrium data and the Langmuir-1 isotherm model showed a high correlation (R 2 ) (Table 2). The Langmuir model assumes homogeneous adsorption energies to the surface, and there is no adsorbate transition on the surface plane [34,[37][38][39][40][41][42][43][44]. The Langmuir equation can be written as:…”

Section: Isotherm and Kineticsmentioning

confidence: 99%

Antibiotic Removal from Aquatic Environment with Activated Carbon Produced from the Pumpkin Seeds

Alacabey¹

2021

Preprint

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Antibiotics are among the most critical environmental pollutant drug groups. One of the methods used to remove this pollution is adsorption. In this study, activated carbon was produced from the pumpkin seed shell and then modified with KOH. This adsorbent obtained was used in the re-moval of ciprofloxacin from aqueous systems. Fourier Transform-Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), elemental, X-ray Photoelectron Spectroscopy (XPS), Brunauer-Emmett-Teller (BET) and Zeta analyzes were used for the characterization of the ad-sorbent. In particular, the surface area was found to be a very remarkable value of 2730 m2/g. The conditions of the adsorption experiments were optimized based on interaction time, adsorbent amount, pH and temperature. Over 99% success has been achieved in removal works carried out under the most optimized conditions. In addition, it was determined that the Langmuir isotherm is the most suitable model for the adsorption interaction.

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“…Cryogels have 3D elastic interconnected macropores, spongy morphologies and good mechanical robustness [3]. They are extremely versatile tools when converted to desired purposes [4] such as purification/adsorption of biomolecules [5][6][7][8], and removal of some pollutants [9][10][11]. Increasing the existing properties while designing cryogels will further increase their potential use in different fields [4].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of xylanase enzyme on poly-(HEMA-co-GMA) cryogel

Noma

2022

MANAS Journal of Engineering

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In this study, a polyethyleneimine (PEI) coated poly-(HEMA-co-GMA), hybrid cryogel column (HCC), was designed. HCC was synthesized via polymerization of gel-former factors at minus temperatures. The characterization experiments of the HCC were conducted through SEM, and FTIR experiments. At the end of the experimental periods, there was no significant decrease in the performance of the HCC. Then the HCC was used as a novel support for xylanase immobilization for the first time. the successful immobilization of xylanase was confirmed by FT-IR, while the biochemical properties and stability of the PHG/PI-Xyl were evaluated in terms of optimum pH, optimum temperature, thermostability, storage stability, reusability, and kinetic parameters, The optimum activities for both free and immobilized enzyme were recorded at pH 6.0, while optimum temperature for free was 55 °C and for PHG/PI-Xyl 60 °C. The PHG/PI-Xyl displayed remarkable thermal stability for 180 min at 60 °C, with 53.55%, and for free Xyl 32.05%. Meanwhile, it retained up to 49% and 69 % for free and immobilized xylanase of original activities after 4 weeks of storage at room temperature. The PHG/PI-Xyl retained about 58% of its original activity after 10 consecutive reuses, while Km for the free Xyl and PHG/PI-Xyl were calculated at 4.05 mg/mL and 2.62 mg/mL, whereas Vmax133.33 U/mL and 188.68 U/mL, respectively. As envisioned, this study suggests a promising way to solve the problems of high price and poor operational stability of the enzyme during biocatalytic.

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Pumice particle interface: a case study for immunoglobulin G purification

Cited by 15 publications

References 42 publications

Antibiotic Removal from the Aquatic Environment with Activated Carbon Produced from Pumpkin Seeds

Antibiotic Removal from the Aquatic Environment with Activated Carbon Produced from Pumpkin Seeds

Antibiotic Removal from Aquatic Environment with Activated Carbon Produced from the Pumpkin Seeds

Immobilization of xylanase enzyme on poly-(HEMA-co-GMA) cryogel

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