PUMA gene transfection can enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells

Cg, Sun; Zhuang, Jing; Wj, Teng; Wang, Z; Ss, Du Plessis

doi:10.4238/2015.may.29.6

Cited by 3 publications

(4 citation statements)

References 5 publications

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Section: Methodsmentioning

confidence: 99%

“…MTT (Nanjing Jiancheng Inc., Nanjing, China) and DMSO (Sigma, St Louis, MI, USA) were used to detect the viability of the cells using a microplate spectrophotometer to measure the 490-nm OD (reference OD of 620 nm) in each well. 27 Flow cytometry BEAS-2B cells in the logarithmic growth phase were cultured; 1-3 Â 10 6 cells were collected, resuspended in 500 lL of apoptosis positive control solution, and incubated on ice for 30 min. Cold binding buffer (1Â; 1.0 mL) was added.…”

Section: Mtt Assaymentioning

confidence: 99%

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Grape seed proanthocyanidin extract alleviates arsenic-induced lung damage through NF-κB signaling

Wang

Niu

et al. 2019

Exp Biol Med (Maywood)

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Proanthocyanidin has beneficial features such as free radical scavenging, anti-inflammation, and anti-oxidation. There is no study on whether proanthocyanidin could protect against arsenic-induced respiratory inflammatory damage. The aim of the study is to examine the anti-inflammatory effects of proanthocyanidin and the molecular mechanisms in vivo and in vitro. BEAS-2B cells were treated with As2O3, grape seed proanthocyanidin extract (GSPE), and/or BAY 11–7082. Kunming mice were treated with As2O3 and/or GSPE. p-IκB-α, IκB-α, IKKα/β, NF-κBp65, and NF-κBp50 were assessed by Western blot and qRT-PCR. Lung histology was examined. Arsenic affected the histology of the mouse lungs, but GSPE attenuated those effects. As2O3 increased cell apoptosis, which was reversed by GSPE. In cells and mouse lung tissue, arsenic increased the expression of IL-1β, IL-6, tumor necrosis factor-α, and C-reactive protein, and these effects were attenuated by GSPE. In cells and mouse lung tissue, arsenic enhanced the mRNA and protein levels of IKKα, IKKβ, NF-κBp65, and NF-κBp50, while the IκB-α levels were decreased compared with controls. IKKα, IKKβ, NF-κBp65, and NF-κBp50 mRNA and protein levels in the As2O3+GSPE groups were lower and IκB-α levels were higher than that in the arsenic groups. Arsenic-activated NF-κB signaling induced inflammatory damage through the upregulation of pro-inflammatory cytokines and downregulation of anti-inflammatory cytokines. GSPE plays a beneficial role against arsenic-induced inflammatory damage through, at least in part, the suppression of the arsenic-induced NF-κB signaling pathway. Impact statement Arsenic-induced respiratory inflammatory damage is an important occupational hazard in many areas of the world, particularly in underdeveloped and developing countries. Effective treatments are lacking and expensive. Therefore, the aim of the study was to examine the anti-inflammatory effects of proanthocyanidin (PC) and the molecular mechanisms in vivo and in vitro. The present study showed that PC extracted from grape seed could attenuate the lung damage in a mouse model of arsenic poisoning. The effects were observed at the level of lung histology and inflammasome expression. This study suggests that a natural compound is effective in mitigating the toxic effects of arsenic in the lungs, providing an inexpensive and more readily accessible method for treating arsenic exposure in some parts of the world.

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Section: Methodsmentioning

confidence: 99%

Section: Mtt Assaymentioning

confidence: 99%

Grape seed proanthocyanidin extract alleviates arsenic-induced lung damage through NF-κB signaling

Wang

Niu

et al. 2019

Exp Biol Med (Maywood)

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“…These are frequently used for breast cancer patients, and the concentrations were determined by referring to previous reports . Three days after the incubation, relative cell viability was calculated as the ratio of treated cells to nontreatment cells the WST‐8 assay …”

Section: Methodsmentioning

confidence: 99%

“…(16)(17)(18) Three days after the incubation, relative cell viability was calculated as the ratio of treated cells to nontreatment cells the WST-8 assay. (19,20) Statistical analysis. CITED2 status and clinicopathological factors were evaluated by a Mann-Whitney U-test or a crosstable using the v 2 -test.…”

Section: Methodsmentioning

confidence: 99%

CITED2 in breast carcinoma as a potent prognostic predictor associated with proliferation, migration and chemoresistance

et al. 2016

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CITED2 (Cbp/p300‐interacting transactivator, with Glu/Asp‐rich carboxy‐terminal domain, 2) is a member of the CITED family and is involved in various cellular functions during development and differentiation. Mounting evidence suggests the importance of CITED in the progression of human malignancies, but the significance of CITED2 protein has not yet been examined in breast carcinoma. Therefore, in the present study, we examined the clinical significance and the biological functions of CITED2 in breast carcinoma by immunohistochemistry and in vitro study. CITED2 immunoreactivity was detected in breast carcinoma tissues, and it was significantly higher compared to those in morphologically normal mammary glands. CITED2 immunoreactivity was significantly associated with stage, pathological T factor, lymph node metastasis, histological grade, HER2 and Ki‐67, and inversely correlated with estrogen receptor. Moreover, the immunohistochemical CITED2 status was significantly associated with increased incidence of recurrence and breast cancer‐specific death of the breast cancer patients, and multivariate analyses demonstrated CITED2 status as an independent worse prognostic factor for disease‐free and breast cancer‐specific survival. Subsequent in vitro experiments showed that CITED2 expression significantly increased proliferation activity and migration property in MCF‐7and S KBR‐3 breast carcinoma cells. Moreover, CITED2 caused chemoresistance to epirubicin and 5‐fluorouracil, but not paclitaxel, in these cells, and it inhibited p53 accumulation after 5‐fluorouracil treatment in MCF‐7 cells. These results suggest that CITED2 plays important roles in the progression and chemoresistance of breast carcinoma and that CITED2 status is a potent prognostic factor in breast cancer patients.

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DNA/RNA-based formulations for treatment of breast cancer

Xie

Zeng

2017

Expert Opinion on Drug Delivery

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To develop a successful formulation for the gene therapy of breast cancer, an effective therapeutic nucleic acid and a proper delivery system are essential. Increased understanding of breast cancer, and developments in biotechnology, material science and nanotechnology have provided a major impetus in the development of effective formulations for the gene therapy of breast cancer. Areas covered: We discuss DNA/RNA-based formulations that can inhibit the growth of breast cancer cells and control the progress of breast cancer. Targets for the gene therapy of breast cancer, DNA/RNA-based therapeutics and delivery systems are summarized. And examples of successful DNA/RNA-based formulations for breast cancer gene therapy are reviewed. Expert opinion: Several challenges remain in developing effective DNA/RNA-based formulations for treatment of breast cancer. Firstly, most of the currently utilized targets are not effective enough as monotherapy for breast cancer. Secondly, the requirements for co-delivery system make the preparation of formulation more complicated. Thirdly, nanoparticles with the modification of tumor-targeting ligands could be more unstable in circulation and normal tissues. Lastly, immune responses against the viral vectors are unfavorable for the gene therapy of breast cancer because of the damage to the host and the impaired therapeutic ability.

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PUMA gene transfection can enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells

Cited by 3 publications

References 5 publications

Grape seed proanthocyanidin extract alleviates arsenic-induced lung damage through NF-κB signaling

Grape seed proanthocyanidin extract alleviates arsenic-induced lung damage through NF-κB signaling

CITED2 in breast carcinoma as a potent prognostic predictor associated with proliferation, migration and chemoresistance

DNA/RNA-based formulations for treatment of breast cancer

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