Pulsed Radiofrequency Neuromodulation Contributes to Activation of Platelet-Rich Plasma in In Vitro Conditions

Michno, Anna; Kirkor, Zbigniew; Gojtowska, Ewelina; Suchorzewski, Marek; Śmietańska, Irmina; Baścik, Bartosz

doi:10.1111/ner.13105

Cited by 6 publications

(5 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, they demonstrated in in vitro samples that the application of low electric field pulses results in a lower generation of platelet microparticles but with overall activation levels similar to those obtained with thrombin [ 29 ]. Recently, Michno et al investigated whether PRF may potentiate the activation of platelets in PRP samples when both these techniques are combined together in in vitro conditions [ 11 ]. They concluded that a combination of both PRF and PRP methods might provide a more effective opportunity for tissue regeneration in several clinical fields.…”

Section: Discussionmentioning

confidence: 99%

“…According to current knowledge, the combination of PRF and PRP can be used in the treatment of nociceptive pain due to peripheral tissue damage [ 10 ]. Michno et al showed that the activation of blood platelets in PRP by PRF potentiated their secretion of alpha and dense granules in in vitro studies [ 11 ]. PRF does not affect platelet integrity, metabolism, and function [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pulsed Radiofrequency and Platelet Rich Plasma in Degenerative Joint Arthritis: Two Case Reports and Literature Analyses

Giaccari

Coppolino

Aurilio

et al. 2023

Life

View full text Add to dashboard Cite

(1) Background: Osteoarthritis (OA) is a debilitating joint disease. The are several therapies available for OA. According to current knowledge, the combination of Platelet-Rich Plasma (PRP) and Pulsed Radiofrequency (PRF) can be applied in the treatment of pain of nociceptive origin due to peripheral tissue damage. (2) Methods: We performed a narrative review identifying the articles by searching electronic databases. A retrospective analysis of patients with OA treated with PRF and PRP in “Vito Fazzi” Hospital (Lecce, Italy) was performed. (3) Results: A total of four publications on the use of PRP and PRF in degenerative joint arthritis were included in our review. In our experience, two patients with OA were treated with PRP and PRF after unsuccessful conservative treatment. Patient pain score, daily activity ability, active range of activity, and muscle strength improved after treatment. Patients reported a higher level of satisfaction. No major adverse events were reported. (4) Conclusions: The goal of the combined application of the two treatments is to make full use of the analgesic effect of PRF and the repairing effect of PRP. At present, the therapeutic potential of PRP and PRF in OA remains unmet.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pulsed Radiofrequency and Platelet Rich Plasma in Degenerative Joint Arthritis: Two Case Reports and Literature Analyses

Giaccari

Coppolino

Aurilio

et al. 2023

Life

View full text Add to dashboard Cite

show abstract

“…Michno et al published an in vitro study using leukocyte-rich PRP (L-PRP) in combination with PRF, and it was observed that PRF was able to activate L-PRP in a similar way that thrombin does. Additionally, the PRF settings used (PRF energy at 500 kHz, at a pulse rate of 5 ms/5 Hz, for a period of 120 s, with an adjusted voltage of 40 V to permit a maximum temperature of 42 °C) had no impact on the functional integrity of platelets and leukocytes present in PRP, suggesting the safety of PRF but only for cellular components of PRP and in vitro conditions [ 103 ]. Aside from pain relief, some in vitro studies have demonstrated that low-frequency pulsed electromagnetic fields could enhance the healing of bone, cartilage, and tendon tissue [ 103 , 104 , 105 ].…”

Section: Radiofrequency Combined With Orthobiologics—current Evidencementioning

confidence: 99%

“…Additionally, the PRF settings used (PRF energy at 500 kHz, at a pulse rate of 5 ms/5 Hz, for a period of 120 s, with an adjusted voltage of 40 V to permit a maximum temperature of 42 °C) had no impact on the functional integrity of platelets and leukocytes present in PRP, suggesting the safety of PRF but only for cellular components of PRP and in vitro conditions [ 103 ]. Aside from pain relief, some in vitro studies have demonstrated that low-frequency pulsed electromagnetic fields could enhance the healing of bone, cartilage, and tendon tissue [ 103 , 104 , 105 ]. Through these studies, it was possible to verify that external physical stimulations could enhance cells to produce extracellular matrix and cytokines for cell proliferation.…”

Section: Radiofrequency Combined With Orthobiologics—current Evidencementioning

confidence: 99%

The Mechanism of Action between Pulsed Radiofrequency and Orthobiologics: Is There a Synergistic Effect?

Jorge¹,

Huber²,

Rodrigues³

et al. 2022

IJMS

View full text Add to dashboard Cite

Radiofrequency energy is a common treatment modality for chronic pain. While there are different forms of radiofrequency-based therapeutics, the common concept is the generation of an electromagnetic field in the applied area, that can result in neuromodulation (pulsed radiofrequency—PRF) or ablation. Our specific focus relates to PRF due to the possibility of modulation that is in accordance with the mechanisms of action of orthobiologics. The proposed mechanism of action of PRF pertaining to pain relief relies on a decrease in pro-inflammatory cytokines, an increase in cytosolic calcium concentration, a general effect on the immune system, and a reduction in the formation of free radical molecules. The primary known properties of orthobiologics constitute the release of growth factors, a stimulus for endogenous repair, analgesia, and improvement of the function of the injured area. In this review, we described the mechanism of action of both treatments and pertinent scientific references to the use of the combination of PRF and orthobiologics. Our hypothesis is a synergic effect with the combination of both techniques which could benefit patients and improve the life quality.

show abstract

“…In the eld of regenerative medicine, there has been increasing interest in utilizing stem cells, PRP injections in various specialties, including dermatology, dentistry, surgery and pain management 9 . PRP, a concentrated solution rich in growth factors secreted by platelets, has been found to contain seven major protein growth factors, such as platelet-derived growth factors (PDGFaa, PDGFab and PDGFbb), transforming growth factors 1 and 2 and vascular endothelial growth factors and epithelial growth factor.…”

Section: Introductionmentioning

confidence: 99%

A new combination of pulsed radiofrequency and platelet-rich plasma injections for management of grade I lumbosacral spondylolisthesis: A prospective study

Le,

Bui,

2024

Preprint

View full text Add to dashboard Cite

Our study aimed to investigate the clinical benefits of combining pulsed radiofrequency (PRF) and platelet-rich plasma (PRP) techniques for treating chronic lumbosacral radicular (CLSR) pain resulting from grade I spondylolisthesis (G1SL). Ninety-six patients with CLSR pain between March 2021 and March 2023 were included in this study. Severity of pain was assessed using the Visual Analog Scale (VAS) score, which measured 6.75 ± 0.98 before procedure and decreased to 3.35 ± 0.78, 3.17 ± 0.56 and 3.03 ± 0.57 at 1, 3 and 6 months after procedure, respectively. Disability-related radicular pain was evaluated using the Oswestry Disability Index (ODI) score, which was 75.84 ± 8.49 before procedure and reduced to 30.19 ± 5.19, 26.01 ± 3.91 and 24.27 ± 3.77 at 1, 3 and 6 months after procedure, respectively. These improvements were statistically significant compared to before procedure (all p < 0.05). The effect was assessed according to the modified MacNab criteria, with 67 cases classified as having an excellent treatment effect. Based on these findings, a new treatment combining PRF and PRP applications appears beneficial in managing pain in regenerative medicine. So, further clinical studies are necessary to support and validate our preliminary findings.

show abstract

Pulsed Radiofrequency Neuromodulation Contributes to Activation of Platelet-Rich Plasma in In Vitro Conditions

Cited by 6 publications

References 40 publications

Pulsed Radiofrequency and Platelet Rich Plasma in Degenerative Joint Arthritis: Two Case Reports and Literature Analyses

Pulsed Radiofrequency and Platelet Rich Plasma in Degenerative Joint Arthritis: Two Case Reports and Literature Analyses

The Mechanism of Action between Pulsed Radiofrequency and Orthobiologics: Is There a Synergistic Effect?

A new combination of pulsed radiofrequency and platelet-rich plasma injections for management of grade I lumbosacral spondylolisthesis: A prospective study

Contact Info

Product

Resources

About