Pulsed plasma deposition of allylamine on polysiloxane: a stable surface for neuronal cell adhesion

Harsch, Annette; Calderon, J.; Timmons, Richard B.; Gross, Guenter W.

doi:10.1016/s0165-0270(00)00196-5

Cited by 115 publications

(85 citation statements)

References 34 publications

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“…46,47 This technology has been successfully employed in variety of biomaterial applications. 44,[48][49][50][51] To investigate the influence of types and densities of surface functional groups on foreign body reactions, polypropylene microspheres were coated with varying densities of -OH and -COOH functionalities. After subcutaneous implantation in mice for two weeks, the extent of inflammatory and fibrotic responses to the implants was then evaluated histologically.…”

Section: Introductionmentioning

confidence: 99%

Species and Density of Implant Surface Chemistry Affect the Extent of Foreign Body Reactions

et al. 2008

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Implant-associated fibrotic capsule formation presents a major challenge for the development of long term drug release microspheres and implantable sensors. Since material properties have been shown to affect in vitro cellular responses and also to influence short term in vivo tissue responses, we have thus assumed that the type and density of surface chemical groups would affect the degree of tissue responses to microsphere implants. To test this hypothesis, polypropylene particles with different surface densities of -OH and -COOH groups, along with the polypropylene control (-CH 2 groups) were utilized. The influence of functional groups and their surface densities on tissue reactions were analyzed using a mice subcutaneous implantation model. Our comparative studies included determination and correlation of the extents of fibrotic capsule formation, cell infiltration into the particles and recruitment of CD11b+ inflammatory cells for all of the substrates employed. We have observed major differences among microspheres coated with different surface functionalities. Surfaces with -OH surface groups trigger the strongest responses while -COOH rich surfaces prompt the least tissue reactions. However, variation of the surface density of either functional group has a relatively minor influence on the extent of fibrotic tissue reactions. The present results show that surface functionality can be used as a powerful tool to alter implant-associated fibrotic reactions and, potentially, to improve the efficacy and function of drug delivery microspheres, implantable sensors and tissue engineering scaffolds.

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Section: Introductionmentioning

confidence: 99%

Species and Density of Implant Surface Chemistry Affect the Extent of Foreign Body Reactions

et al. 2008

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“…After 14 days of cultivation confluent cell growth was observed on the aminocontaining coatings, while on the TMOS-coated plastic surface the cells have detached. The supporting effect of nitrogen-rich plasma polymer films on cell cultivation is already described in the literature [10,29] and is most probably due to positively charged protonated amino groups which can interact with negatively charged biomolecules at physiological pH.…”

Section: Cell Cultivationmentioning

confidence: 80%

“…Successful cultivation of these cells requires very specific conditions with suitably functionalized surfaces providing fertile grounds for the cells. Such surfaces are currently fabricated using various methods including wet-chemical processes [1] modifying the surfaces by polyelectrolyte multilayer (PEM) [2,3] or self-assembly monolayer (SAM) [4,5] coatings, UV-ozone treatment [6][7][8] and plasma processes [9][10][11][12], respectively.…”

Section: Introductionmentioning

confidence: 99%

Surface modification of closed plastic bags for adherent cell cultivation

Lachmann

Dohse

Thomas

et al. 2011

Eur. Phys. J. Appl. Phys.

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To cite this version: Abstract:In modern medicine human mesenchymal stem cells are becoming increasingly important.However, a successful cultivation of this type of cells is only possible under very specific conditions. Of great importance, for instance, are the absence of contaminants such as foreign microbiological organisms, i.e. sterility, and the chemical functionalization of the ground on which the cells are grown. As cultivation of these cells makes high demands, a new procedure for cell cultivation has been developed in which closed plastic bags are used. For adherent cell growth chemical functional groups have to be introduced on the inner surface of the plastic bag. This can be achieved by a new, atmospheric-pressure plasma based method presented in this paper. The method which was developed jointly by the Fraunhofer IST and the Helmholtz HZI can be implemented in automated equipment as is also shown in this contribution.Plasma process gases used include helium or helium-based gas mixtures (He + N 2 + H 2 ) and vapours of suitable film-forming agents or precursors such as APTMS, DACH, and TMOS in helium. The effect of plasma treatment is investigated by FTIR-ATR spectroscopy as well as surface tension determination based on contact angle measurements and XPS spectroscopy.Plasma treatment in nominally pure helium increases the surface tension of the polymer foil due to the presence of oxygen traces in the gas and oxygen diffusing through the gaspermeable foil, respectively, reacting with surface radical centres formed during contact with the discharge. Primary amino groups are obtained on the inner surface by treatment in mixtures with nitrogen and hydrogen albeit their amount is comparably small due to diffusion of oxygen through the gas-permeable bag, interfering with the plasma-amination process.Surface modifications introducing amino groups on the inner surface turned out to be most efficient in the promotion of cell growth.3

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“…Pulsed mode here for the purpose of reducing total energy input to prevent possible damage and control the chemical properties by providing controllable gas phase reactions as well as gas-surface interactions [26]. N 2 alone and N 2 /H 2 (15%) were used as gas sources, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…To resolve the problem, pulsed mode is used to assist CW mode plasma. Pulsed plasma allows a high degree of control over the physical and chemical properties of the thin film deposited by providing control of the gas phase reactions and the gas-surface interactions [26]. Here, we investigated the use of continuous wave plus pulsed (CW+P) mode plasma to control wettability change as well as enhance the quantity of N-functional groups grafted onto BNNTs.…”

mentioning

confidence: 99%

Surface wetting processing on BNNT films by selective plasma modes

Liu

Dai

et al. 2013

Chin. Sci. Bull.

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The wettability of boron nitride nanotube (BNNT) films was modified using a combination of pulsed and continuous wave (CW) mode plasma. The combined mode effectively modified the wettability of BNNT films and kept the nanostructures intact. The BNNT films changed from superhydrophobic to superhydrophilic after combined mode treatment at 600 W min. In contrast, the contact angle controllable decreased linearly in a controllable way with increasing energy input before eventually becoming superhydrophilic after 1000 W min of CW mode treatment. A high concentration of graft functional groups formed, along with point defects. More point defects formed when using combined modes and higher energy input. Mainly amine functional groups were grafted by combined mode plasma, while the CW mode plasma led to more formation of amide and imine on the BNNTs. Research into controllable wettability and selection of grafted functional groups should enable promising applications of BNNTs in composites and biology in the future. boron nitride nanotube, wettability, plasma Citation:Li L, Liu X W, Dai X J J, et al. Surface wetting processing on BNNT films by selective plasma modes.

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Pulsed plasma deposition of allylamine on polysiloxane: a stable surface for neuronal cell adhesion

Cited by 115 publications

References 34 publications

Species and Density of Implant Surface Chemistry Affect the Extent of Foreign Body Reactions

Species and Density of Implant Surface Chemistry Affect the Extent of Foreign Body Reactions

Surface modification of closed plastic bags for adherent cell cultivation

Surface wetting processing on BNNT films by selective plasma modes

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