Pulsed Nano-Electrospray Ionization: Characterization of Temporal Response and Implementation with a Flared Inlet Capillary

Bushey, Jared M.; Kaplan, Desmond; Danell, Ryan M.; Glish, Gary L.

doi:10.1080/10739140902831313

Cited by 32 publications

(32 citation statements)

References 26 publications

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“…The standard glass transfer capillary was replaced with a glass transfer capillary with a custom flared end [25]. The DIMS assembly can be threaded onto the Apollo I source of the Bruker Esquire 3000 ion trap mass spectrometer in place of the spray shield.…”

Section: Methodsmentioning

confidence: 99%

Improved Differential Ion Mobility Separations Using Linked Scans of Carrier Gas Composition and Compensation Field

Santiago

Harris

Isenberg

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. Differential ion mobility spectrometry (DIMS) separates ions based on differences in their mobilities in low and high electric fields. When coupled to mass spectrometric analyses, DIMS has the ability to improve signal-to-background by eliminating isobaric and isomeric compounds for analytes in complex mixtures. DIMS separation power, often measured by resolution and peak capacity, can be improved through increasing the fraction of helium in the nitrogen carrier gas. However, because the mobility of ions is higher in helium, a greater number of ions collide with the DIMS electrodes or housing, yielding losses in signal intensity. To take advantage of the benefits of helium addition on DIMS separations and reduce ion losses, linked scans were developed. In a linked scan the helium content of the carrier gas is reduced as the compensation field is increased. Linked scans were compared with conventional compensation field scans with constant helium content for the protein ubiquitin and a tryptic digest of bovine serum albumin (BSA). Linked scans yield better separation of ubiquitin charge states and enhanced peak capacities for the analysis of BSA compared with compensation field scans with constant helium carrier gas percentages. Linked scans also offer improved signal intensity retention in comparison to compensation field scans with constant helium percentages in the carrier gas.

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Section: Methodsmentioning

confidence: 99%

Improved Differential Ion Mobility Separations Using Linked Scans of Carrier Gas Composition and Compensation Field

Santiago

Harris

Isenberg

et al. 2015

J. Am. Soc. Mass Spectrom.

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“…The ratio of helium to nitrogen in the carrier gas was varied using two MKS model 1179 mass flow controllers regulated by a LabVIEW program and routed into the desolvation gas line. The standard glass transfer capillary of the source was replaced with a custom flared glass transfer capillary [20]. …”

Section: Methodsmentioning

confidence: 99%

Optimization of Peptide Separations by Differential Ion Mobility Spectrometry

Isenberg

Armistead

Glish

2014

J. Am. Soc. Mass Spectrom.

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Differential ion mobility spectrometry (DIMS) has the ability to separate gas phase ions based on their difference in ion mobility in low and high electric fields. DIMS can be used to separate mixtures of isobaric and isomeric species indistinguishable by mass spectrometry (MS). DIMS can also be used as a filter to improve the signal-to-background of analytes in complex samples. The resolving power of DIMS separations can be improved several ways, including increasing the dispersion field and increasing the amount of helium in the nitrogen carrier gas. It has been previously demonstrated that the addition of helium to the DIMS carrier gas provides improves separations when the dispersion field is the kept constant as helium content is varied. However, helium has a lower breakdown voltage than nitrogen. Therefore, as the percent helium content in the nitrogen carrier gas is increased, the highest dispersion field accessible decreases. This work presents the trade-offs between increasing dispersion fields and using helium in the carrier gas by comparing the separation of a mixture of isobaric peptides. The maximum resolution for a separation of a mixture of three peptides with the same nominal molar mass was achieved by using a high dispersion field (~72 kV/cm) with pure nitrogen as the carrier gas within the DIMS assembly. The conditions used to achieve the maximum resolution also exhibit the lowest ion transmission through the assembly, suggesting that it is necessary to consider the trade-off between sensitivity and resolution when optimizing DIMS conditions for a given application.

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“…145 Dedicated high-voltage switches and transformers can be used to drive finer electrospray ionization (ESI) modes through high-voltage pulses, dielectric barrier polarization, or capacitive induction. [146][147][148][149][150] Based on the fine and accurate characteristics of the charge pulse, the ionization process that triggers the more durable ESI device can be repetitive and simple to operate. 151 Nano-ESI due to the high voltage of TENG can bring higher sensitivity under the charge of lower ion pulse, so as to maximize the utilization of the sample; this is a matter of improving efficiency on both sides.…”

Section: Microactuators Triggered By Tengsmentioning

confidence: 99%

High-voltage applications of the triboelectric nanogenerator—Opportunities brought by the unique energy technology

Wang

et al. 2019

MRS Energy & Sustainability

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Pulsed Nano-Electrospray Ionization: Characterization of Temporal Response and Implementation with a Flared Inlet Capillary

Cited by 32 publications

References 26 publications

Improved Differential Ion Mobility Separations Using Linked Scans of Carrier Gas Composition and Compensation Field

Improved Differential Ion Mobility Separations Using Linked Scans of Carrier Gas Composition and Compensation Field

Optimization of Peptide Separations by Differential Ion Mobility Spectrometry

High-voltage applications of the triboelectric nanogenerator—Opportunities brought by the unique energy technology

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