Pulsed Laser Diode-Based Desktop Photoacoustic Tomography for Monitoring Wash-In and Wash-Out of Dye in Rat Cortical Vasculature

Abstract

Pulsed laser diode based desktop photoacoustic tomography for monitoring wash-in and wash-out of dye in rat cortical vasculature

Recent advances in photoacoustic contrast agents for in vivo imaging

“…In the NIR region (780–2,500 nm; Lu & Fei, ) the tissue attenuation and scattering is low; hence these wavelengths are ideal for deep‐tissue PAI (He, Song, Qu, & Cheng, ; Smith, Mancini, & Nie, ). The NIR‐I window (780–1,100 nm) was extensively investigated for deep‐tissue and small animal imaging (Kalva, Upputuri, & Pramanik, ; Kalva, Upputuri, Rajendran, Dienzo, & Pramanik, ; K. H. Song, Kim, Cobley, Xia, & Wang, ; P. K. Upputuri & Pramanik, ; Z. Wu et al, ; X. Yang, Skrabalak, Li, Xia, & Wang, ; Y. Zhou, Yao, & Wang, ). Due to the reduced blood absorption in the NIR region, the imaging depth is improved with a small sacrifice in contrast.…”

“…In the NIR region (780-2,500 nm; Lu & Fei, 2014) the tissue attenuation and scattering is low; hence these wavelengths are ideal for deep-tissue PAI (He, Song, Qu, & Cheng, 2018;Smith, Mancini, & Nie, 2009). The NIR-I window (780-1,100 nm) was extensively investigated for deep-tissue and small animal imaging Kalva, Upputuri, Rajendran, Dienzo, & Pramanik, 2019;K. H. Song, Kim, Cobley, Xia, & Wang, 2009; P. K. Upputuri & Pramanik, 2015;Z.…”

In vivo detection of venous sinus distension due to intracranial hypotension in small animal using pulsed‐laser‐diode photoacoustic tomography

“…It is due to its lower cost, compact size and high repetition rate in comparison with the traditional Nd:YAG‐based laser excitation sources. PLD‐based PAT system for various applications such as brain imaging, monitoring wash in and wash out of contrast agents, diagnosis of cardiovascular diseases, and diagnosis of rheumatological disorders have been demonstrated already. Use of acoustic reflector‐based single‐element UTR (SUTR) instead of the traditionally used horizontal UST's makes the PLD‐PAT system even more compact by reducing the scanning radius.…”

Monte Carlo simulations and photoacoustic experiments to compare imaging depth at 532 nm, 800 nm, and 1064 nm

“…Being a hybrid imaging modality, PAI provides the advantages of good optical contrast and high ultrasound resolution making it a promising tool for pre-clinical and clinical applications [5][6][7]. This imaging modality has therefore been employed for various applications such as sentinel lymph node (SLN) imaging [8], small animal whole body imaging [9], breast imaging [10,11], brain imaging [12,13], monitoring of hemoglobin concentration [14] etc. PAI can be broadly classified as photoacoustic microscopy (PAM) and photoacoustic tomography/photoacoustic computed tomography (PAT/PACT) [15,16].…”