Pulsed laser deposition of polyhydroxybutyrate biodegradable polymer thin films using ArF excimer laser

Kecskeméti, Gabriella; Smausz, Tomi; Kresz, N.; Tóth, Z.; Hopp, B.; Chrisey, Douglas B.; Berkesi, Ottó

doi:10.1016/j.apsusc.2006.01.084

Cited by 22 publications

(14 citation statements)

References 18 publications

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“…Production of biosensors often requires immobilization of different active biomaterials in form of thin films on the appropriate transducer devices. Pulsed Laser Deposition (PLD) found to be effective in preparation of inorganic thin layers was proven to be hardly applicable for deposition of complex organic materials, especially the sensitive biomaterials, where -if applicable -low laser fluences, near to ablation threshold have to be applied resulting in very low deposition rates [1][2][3][4]. To avoid the photonic and thermal damages of these materials during the thin film deposition the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique was developed, where the target material is embedded in an absorptive volatile matrix.…”

Section: Introductionmentioning

confidence: 99%

Comparative study on Pulsed Laser Deposition and Matrix Assisted Pulsed Laser Evaporation of urease thin films

et al. 2009

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Section: Introductionmentioning

confidence: 99%

Comparative study on Pulsed Laser Deposition and Matrix Assisted Pulsed Laser Evaporation of urease thin films

et al. 2009

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“…The mean value of the background (BG) signal was subtracted from the mean value of the corresponding PA signal measured in aerosol-containing gas. Taking the BG-corrected PA data and the determined mass concentration value obtained by weighing the aerosols accumulated on the filter, optical absorption and mass specific absorption cross section were derived at the four applied wavelengths according to (1). The measured optical absorptions, σ abs , and their wavelength dependences at the investigated fluences are shown in Fig.…”

Section: Size Distributionmentioning

confidence: 99%

“…The pulsed laser deposition (PLD) technique is a promising procedure for realization of such layers. Previous experiments demonstrated that under appropriate laser parameters the procedure was successfully applied for various biomaterials such as biodegradable polymers [1,2], enzymes [3][4][5][6][7], proteins [8][9][10], collagen [11], etc. where the deposited layers retain the biological properties and functionality of the target material.…”

Section: Introductionmentioning

confidence: 99%

Characterization of excimer laser ablation generated pepsin particles using multi-wavelength photoacoustic instrument

et al. 2012

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Preparation of organic thin layers on various special substrates using the pulsed laser deposition (PLD) technique is an important task from the point of view of bioengineering and biosensor technologies. Earlier studies demonstrated that particle ejection starts during the ablating laser pulse resulting in significant shielding effects which can influence the real fluence on the target surface and consequently the efficiency of layer preparation. In this study, we introduce a photoacoustic absorption measurement technique for in-situ characterization of ablated particles during PLD experiments. A KrF excimer laser beam (λ = 248 nm, FWHM = 18 ns) was focused onto pepsin targets in a PLD chamber; the applied laser fluences were 440 and 660 mJ/cm 2 . We determined the wavelength dependence of optical absorption and mass specific absorption coefficient of laser ablation generated pepsin aerosols in the UV-VIS-NIR range. On the basis of our measurements, we calculated the absorbance at the ablating laser wavelength, too. We demonstrated that when the laser ablation generated pepsin aerosols spread through the whole PLD chamber the effect of absorptivity is negligible for the subsequent pulses. However, the interaction of the laser pulse and the just formed particle cloud generated by the same pulse is more significant.B

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“…A number of experiments have shown that the nanosecond PLD technique is suitable for deposition of different types of biocompatible polymers [5][6][7][8][9][10][11] and for materials found in the mammalian organism, such as hydroxyapatite, dentine and pepsin [12][13][14]. The main drawback of the nanosecond PLD is the possible thermal or photochemical damage of the sensitive biological samples.…”

Section: Introductionmentioning

confidence: 99%

Directed Cell Growth on Laser-Transferred 2D Biomaterial Matrices

Nógrádi¹,

Hopp²,

Smausz³

et al. 2008

TOTERMJ

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Patterned arrangement of living cells to form tissues on a given surface is the prerequisite of successful bioengineering, tissue building and biosensor technology. The aim of the present study was to deposit various biomaterials onto a given substrate to establish a patterned cellular matrix using laser-based technologies. We used the Pulsed Laser Deposition and the Absorbing Film-Assisted Laser-Induced Forward Transfer methods to deposit various biomaterials, such as fibronectin, collagen and endothelial cell growth supplement. Subpicosecond KrF excimer laser was used for irradiation of the target materials. When cultured neuroectodermal stem cells, astrocytes, endothelial and neuroblastoma cells were layered on the deposited biomaterial patterns a guided growth of these cells was induced along the patterned thin film. Some cell types showed various interactions when approached each other. The above methods are suitable to build an architecture of substrates which supports and guides the growth of cells and may enable the cells to induce directed and rapid repair of injured tissues.

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Pulsed laser deposition of polyhydroxybutyrate biodegradable polymer thin films using ArF excimer laser

Cited by 22 publications

References 18 publications

Comparative study on Pulsed Laser Deposition and Matrix Assisted Pulsed Laser Evaporation of urease thin films

Comparative study on Pulsed Laser Deposition and Matrix Assisted Pulsed Laser Evaporation of urease thin films

Characterization of excimer laser ablation generated pepsin particles using multi-wavelength photoacoustic instrument

Directed Cell Growth on Laser-Transferred 2D Biomaterial Matrices

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