Pulsed laser deposition and in vitro characteristics of triphasic – HASi composition on titanium

Abstract

Pulsed laser deposition was used to deposit bioactive triphasic glass-ceramic composition (HASi) over titanium substrate using dense HASi target. Bioactive glass compositions are considered the most useful synthetic materials for immediate bone attachment because of its bioresorption, osteoconduction and osteointegration characteristics under in vivo conditions. The disadvantage of its brittleness associated with bioactive glass-ceramics has prompted its coating over metallic implants for the combination of duo mechanical and bioactive properties. The hard HASi target was able to undergo laser ablation under ambient gas pressure without bulk erosion of the target. Laser deposition was found to be efficient in depositing triphasic composition for immediate bone integration. The target and deposits were analyzed for the phase, composition and microstructural characteristics by means of X-ray diffraction, Fourier transform infrared spectroscopy, energy-dispersive X-ray analysis and scanning electron microscopy. Simultaneously, the adherent nature and mechanical behaviour of deposits were confirmed by scratch test and micro-indentation methods. Further, the in vitro dissolution and bioactivity were assessed by soaking in simulated body fluid followed by elemental analysis using inductively coupled plasma spectroscopy. The deposits were found to be cell-friendly, which was indicated by the phenomenology of stem cells under in vitro conditions.

3D‐bulk to nanoforms of modified hydroxyapatite: Characterization and osteogenic potency in an in vitro 3D bone model system

“…The peaks at 25.2, 31.1, 31.4° in SrHA matched the standard patterns of JCPDS No. 034‐0479 24,31 . HASi has a major phase of HA with an outer coating of HA, tricalcium phosphate and calcium silicate—a similar XRD pattern to that of HA.…”

“…The varied therapeutic scenario of non‐load bearing skull bone defects to finger joints demands the development of new nano‐bioceramic implants for stem cell loading and drug release‐based tissue regeneration. Our earlier studies have devised the development of novel in‐house synthesized biocomposite materials 22–26 for tissue regenerative applications ranging from small to large defects with successful outcome in pre‐clinical models. Hence this study was a comparative validation of four different 3D bulk bioactive ceramic materials HA, BCP, SrHA and HASi in an in vitro 3D bone model system incorporating RADMSCs.…”

Modification of titanium orthopedic implants with bioactive glass: a systematic review of in vivo and in vitro studies

“…Mesenchymal matrix or stem cells, which are derived from a variety of tissues such as the bone marrow and adipose tissue, are multipotent adult cells ( Zhang et al, 2016 ) and can differentiate into different cell lines. Adipose-derived mesenchymal stem cells (MSCs) are more frequently used because they are abundant and the collection process is simpler and does not cause great trauma ( Chen et al, 2014 ), and this leads to their wide application prospects in regenerative medicine ( Palangadan et al, 2014 ). Such MSCs can also be used to study the osteogenic differentiation potential of glass materials and to study the ability to synthesize certain specific proteins ( Rau et al, 2020 ).…”

“…Coatings formed by PLD have a dense surface, a better chemical composition ratio of BG ( Ledda et al, 2016 ), and a microsphere ( Palangadan et al, 2014 ). The nanostructure on the surface can help in implant osteointegration ( Rau et al, 2020 ).…”