Pulsed growth of fungal hyphal tips.

López-Franco, Rosamarı́a; Bartnicki-García, S.; Bracker, Charles E.

doi:10.1073/pnas.91.25.12228

Cited by 140 publications

(128 citation statements)

References 27 publications

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“…Both the temporal resolution (1 s) and length of time series (~25-100 period lengths) in our data are more than adequate to reveal previously reported pulsatile growth. Furthermore, our best spatial resolution of about 1 pixel s 21 equals 1?8 mm min 21 , less than the reported amplitudes and the same as the pixel size used previously (Ló pez-Franco et al, 1994). Alternatively, with a mean growth rate of 15 mm min 21 , a typical rate (Table 1), the growth increment is 7 pixels s 21 , well above the repeatability of 1 pixel determined for the location of the tip at each measurement point.…”

Section: Discussion Technical Considerationsmentioning

confidence: 83%

“…As previously observed (Ló pez-Franco et al, 1994), many older reports of hyphal tip growth simply lacked sufficient temporal or spatial resolution to detect pulsatile growth. In this regard, the report by Lopez-Franco et al (1994) served a very useful purpose, emphasizing that relatively fast changes in hyphal growth rate can occur.…”

Section: Possible Explanations Of Previous Datamentioning

confidence: 95%

“…Previous reports (Introduction) have defined the range of period lengths and amplitudes (trough to peak) found in tip-growing systems as being about 4-60 s and 1?6-15 mm min 21 , respectively. More specifically, the reported values for N. crassa and S. ferax, the hyphae examined in the present work, are periods of 4?7 and 5?1 s and growth rates of 5?6 and 2?2 mm min 21 , respectively (Ló pez-Franco et al, 1994). We examined these two organisms as both are used routinely in our laboratories.…”

Section: Discussion Technical Considerationsmentioning

confidence: 99%

“…More recently, pulsed growth was reported for diverse fungi and oomycetes (Ló pez-Franco et al, 1994), described as fluctuating ''continuously with alternating periods of fast and slow growth at more or less regular intervals''. The published datasets were limited to short time durations and not analysed statistically.…”

Section: Introductionmentioning

confidence: 99%

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Time series analysis demonstrates the absence of pulsatile hyphal growth

2003

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Hyphal tip growth has been previously reported as pulsatile, defined as regularly alternating fast and slow rates of extension. The growth of pollen tubes, and hyphae of Neurospora crassa and Saprolegnia ferax were analysed using high spatial and temporal resolution. By using long (100-500 s) records of growth rate, sampled every second, it was possible to apply rigorous statistical analysis of the time series. As previously demonstrated, pollen tubes can show pulsatile growth, detectable with this system. In contrast, hyphal growth rates do not show any evidence of pulsatile growth; instead, growth rates appear to fluctuate randomly. It is concluded that pulsatile growth is not a common feature of hyphal tip growth.

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Section: Discussion Technical Considerationsmentioning

confidence: 83%

Section: Possible Explanations Of Previous Datamentioning

confidence: 95%

Section: Discussion Technical Considerationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Time series analysis demonstrates the absence of pulsatile hyphal growth

2003

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“…Подовження гіф у грибів відбувається внаслідок росту їх кінчиків, тобто для них характерний апікальний характер росту [3,7,16,18]. Міцелій покриває твер де середовище та засвоює з нього поживні речовини значно ефективніше, ніж, наприклад, колонії бактерій чи дріжджів.…”

Section: к л ю ч о в і с л о в а: швидкість радіального росту мікромunclassified

Growth of Micromycetes from Different Ecological Niches on Agar Nutrient Media

Kurchenko¹,

Yurieva²,

Voychuk³

2015

Mikrobiol. Z.

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Imaging Spitzenkörper, pH and calcium dynamics in growing fungal hyphae

1998

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Plant diseases caused by Ðlamentous fungi result in the loss of crops worth billions of pounds per year. Agrochemical companies are therefore continually trying to discover and develop new and e †ective antifungal compounds which prevent or inhibit the growth of fungal pathogens in order to improve crop quality and yield.1 The mechanistic basis of the growth of Ðlamentous fungi is currently the focus of much research. A major challenge in the future will be to discover novel antifungal targets within the multifactorial process of fungal growth.Tip growth is the main way by which fungi grow, involving the polarized extension of a hypha by means of localized secretion and cell-wall synthesis at the hyphal apex.2,3 In Edinburgh we are using confocal microscopy with a range of vital Ñuorescent probes to analyse hyphal tip growth and its regulation in living cells.4,5 Three aspects being concentrated on are : (1) Spitzenko rper behaviour ; (2) intracellular pH ; and (3) intracellular Ca2`.Growing hyphal tips contain a multi-component organelle assemblage called the Spitzenko rper which is assumed to contain the secretory vesicles responsible for tip growth. Spitzenko rper behaviour is associated intimately with the dynamic growth pattern and morphogenesis of the hyphal tip.5h11 We have developed a Ñuorescent staining procedure which allows the dynamic behaviour of the Spitzenko rper to be visualised (in growing hyphae) for the Ðrst time.5The Spitzenko rper-selective dye we use is FM4-64. This styryl dye stains components of the endocytotic pathway and also, after membrane recycling, exocytotic vesicles. FM4-64 (and related dyes especially FM1-43) has been extensively used to image and analyse membrane trafficking during endocytosis and exocytosis in animal cells,12 and more recently has been used to study endocytosis in yeast.13,14 Using this dye we have stained the Spitzenko rper in growing hyphae of a wide range of Ðlamentous fungi including Aspergillus niger. v.

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Pulsed growth of fungal hyphal tips.

Cited by 140 publications

References 27 publications

Time series analysis demonstrates the absence of pulsatile hyphal growth

Time series analysis demonstrates the absence of pulsatile hyphal growth

Growth of Micromycetes from Different Ecological Niches on Agar Nutrient Media

Imaging Spitzenkörper, pH and calcium dynamics in growing fungal hyphae

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