Pulsed-field gel electrophoresis (PFGE) as an epidemiological marker for typing of methicillin-resistant Staphylococcus aureus recovered from King Saudi Arabia (KSA)

Abstract

In this study, pulsed field gel electrophoresis (PFGE) was used for genomic DNA finger printings of methicillin-resistant Staphylococcus aureus (MRSA) isolates. Thirty strains of S. aureus collected from major hospital laboratories and public health centers, Riyadh, King Saudi Arabia were tested phenotypically by conventional methods and genotypically by multiplex-PCR for direct detection of S. aureus 16S rRNA and mecA genes. The chromosomal DNA of the isolates was examined by using pulsed-field gel electrophoresis (PFGE) using SmaI. SmaI cut the chromosomal DNA of the examined MRSA into 9 to 13 fragments, moreover, 16 chromosomal digestion patterns were observed out of the 30 examined isolates. The first pulsed field gel electrophoresis (PFGE1) contains 9 strains recovered from soft tissue infections and surgical wound infections. The second one (PFGE 2) contains 4 MRSA isolates, 3 of which were recovered from skin and soft tissue infections, while one was recovered from wound infection. Moreover, there are 3 chromosomal digestion patterns (PFGE 3, 4 and 5), each pattern involved two strains of MRSA which were recovered from surgical wound infections. A dendrogram of percent similarity, revealed three major clusters, the first cluster containing four groups (17 strains). The second cluster contains one group (12 strains), while the third cluster contains only one strain recovered from deep abscess.

3D‐printed chip for detection of methicillin‐resistant Staphylococcus aureus labeled with gold nanoparticles

“…D). Moussa and Shibl also used 16S rRNA and mecA genes for identification of MRSA . Their study was expanded to include the presence of Panton‐Valentine Leucocidin, which is a cytotoxin produced by community‐associated MRSA bacterial strain , and are in good agreement with those found in our study.…”

Methicillin-Resistant Staphylococcus Aureus in Saudi Arabia: Genotypes distribution review

“…The first, which included 17 strains were subdivided into four groups, and the second consisted of 12 strains, while the third cluster contained only one strain. [25]…”

Characterization of Staphylococcus aureus isolated from human dental infection

“…The propensity of S. aureus to acquire antibiotic resistance has prompted worldwide dissemination of clone expressing various antimicrobial resistances. Several hospital and nonhospital bacterial diseases are caused due to MRSA strains and sometimes lead to death (Bannerman and Peacock, 2007;Moussa et al, 2011;Peters et al, 2013;Faden, 2018).…”