Pulsed-field gel electrophoresis-based subtyping of DNA degradation-sensitive Salmonella enterica subsp. enterica serovar Livingstone and serovar Cerro isolates obtained from a chicken layer farm

Murase, Toshiyuki; Nagato, Masashi; Shirota, Kinya; Katoh, Hiromitsu; Otsuki, Koichi

doi:10.1016/j.vetmic.2003.11.014

Cited by 39 publications

(23 citation statements)

References 15 publications

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“…Pulsed-field gel electrophoresis (PFGE) is a molecular typing method that was adapted to Salmonella in the 1990s (30,60,64,89). The technique is useful for fingerprinting strains in outbreak situations and is relatively inexpensive to perform.…”

Section: Molecular Typing Techniquesmentioning

confidence: 99%

Methodologies for Salmonella enterica subsp. enterica Subtyping: Gold Standards and Alternatives

Wattiau

Boland

Bertrand

2011

Appl Environ Microbiol

221

203

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2For more than 80 years, subtyping of Salmonella enterica has been routinely performed by serotyping, a method in which surface antigens are identified based on agglutination reactions with specific antibodies. The serotyping scheme, which is continuously updated as new serovars are discovered, has generated over time a data set of the utmost significance, allowing long-term epidemiological surveillance of Salmonella in the food chain and in public health control. Conceptually, serotyping provides no information regarding the phyletic relationships inside the different Salmonella enterica subspecies. In epidemiological investigations, identification and tracking of salmonellosis outbreaks require the use of methods that can fingerprint the causative strains at a taxonomic level far more specific than the one achieved by serotyping. During the last 2 decades, alternative methods that could successfully identify the serovar of a given strain by probing its DNA have emerged, and molecular biology-based methods have been made available to address phylogeny and fingerprinting issues. At the same time, accredited diagnostics have become increasingly generalized, imposing stringent methodological requirements in terms of traceability and measurability. In these new contexts, the hand-crafted character of classical serotyping is being challenged, although it is widely accepted that classification into serovars should be maintained. This review summarizes and discusses modern typing methods, with a particular focus on those having potential as alternatives for classical serotyping or for subtyping Salmonella strains at a deeper level.

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Section: Molecular Typing Techniquesmentioning

confidence: 99%

Methodologies for Salmonella enterica subsp. enterica Subtyping: Gold Standards and Alternatives

Wattiau

Boland

Bertrand

2011

Appl Environ Microbiol

221

203

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“…The digested genomic DNA of target bacteria is separated on an agarose gel and then hybridized with complementary sequences for identifying the banding patern. A database of ingerprint species, serovar, and strain identiications is used for comparison [21][22][23]. The ingerprinting methods include pulsed-ield gel electrophoresis (PFGE), ribotyping, and intergenic sequence (IGS) ribotyping.…”

Section: Culture-dependent Methodsmentioning

confidence: 99%

Current and Emerging Innovations for Detection of Food-Borne Salmonella

Wu¹,

Zeng²

2017

Current Topics in Salmonella and Salmonellosis

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Salmonella is one of the leading causes of food-borne illnesses worldwide, and one of the main contributors to salmonellosis is the consumption of contaminated egg, poultry, pork, beef, and milk products. Since deleterious efects of Salmonella on public health and the economy continue to occur, improving safety of food products by early detection of food-borne pathogens would be considered an important component for limiting exposure to Salmonella contamination. Therefore, there is an ongoing need to develop more advanced detection methods that can identify Salmonella accurately and rapidly in foods before they reach consumers. In the past three decades, there have been increasing eforts toward developing and improving rapid pathogen detection and characterization methodologies for application to food products. In this chapter, we discuss molecular methods for detection, identiication, and genetic characterization of Salmonella in food. In addition, the advantages and disadvantages of the established and emerging rapid detection methods are addressed here. The methods with potential application to the industry are highlighted in this chapter.

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“…Carril 4,6-7) no se diferenciaron utilizando PFGE, por la restricción total o degradación del ADN genómico probablemente debido a la presencia de ADNasas, señalando a S. enterica serovar Javiana como no tipificable por PFGE. Las cepas en las que los patrones o pulsotipos diferentes corresponden a los diferentes serotipos Salmonella Agona, Meleagridis, E1, Antigeno Flagelar Rugoso, Anatum, Sinstorf, Javiana, Urbana, Typhimurium, London, Give, Enteritidis, Montevideo, Braenderup y Braenderup H9812 respectivamente (Ribot et al, 2006;Wong et al, 2006;Murase et al, 2004). El análisis de macrorestricción en PFGE presentó un índice de discriminación mayor (D=0.95), superior al de la serotipificación (D=0.86).…”

Section: I I Ii Ii Ii Ii Ii Iii Iii Ii I Ii Iii I Iv V Vi Vii Viii unclassified

“…En otro caso, el serotipo S. Javiana no proporciono un pulsotipo visible o distinguible, probablemente por la degradación del ADN, debido a ADNasas; aparte de la degradación del ADN, la metilación de ADN genómico, es debido a que la actividad enzimática de Xba I, es bloqueada por Metilasas en las secuencias de reconocimiento. (Tenover, et al, 2006;Ribot, et al, 2006;Murase et al, 2004;Fendri et al, 2013;Wong et al, 2006).…”

Section: I I Ii Ii Ii Ii Ii Iii Iii Ii I Ii Iii I Iv V Vi Vii Viii unclassified

“…La secuenciación del genoma por su costo no puede ser utilizada en estudios epidemiológicos amplios, por lo tanto, PFGE ha sido considerado como el estándar de oro entre los métodos de caracterización molecular por poseer una alta reproducibilidad y un alto poder de discriminación. Además, es el primer método de elección en los laboratorios de PulseNet International, para la vigilancia e investigación de brotes epidemiológicos de bacterias patógenas clínicamente importantes (Lukinmaa et al, 2004;Murchan et al, 2003;Valdez et al, 2015;Sabat et al, 2013;Sandt et al, 2006;Murase et al 2004). La electroforesis de ADN convencional es una técnica extraordinariamente simple: un campo eléctrico constante es aplicado a través de una placa de gel de agarosa y las moléculas de ADN pequeñas son tamizadas y migran en una forma dependiente del tamaño, pero el efecto de tamizado y de separación falla cuando moléculas de ADN de mayor tamaño deben ser resueltas.…”

Section: Introductionunclassified

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Caracterización molecular de bacterias patógenas mediante electroforesis en gel de campos pulsados (PFGE)

et al. 2016

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The growing need for surveillance of re-emerging infectious diseases necessitates the application of molecular characterization methods such as Pulsed-Field Gel Electrophoresis -PFGE. In the first collection, analysis macrorestriction S. aureus reveals a medium frequency (0.54) of pulsetype II, compared to the second collection where increased genetic heterogeneity exists. S. enterica presented some identical pulsotypes and other pulsotypes presented a genetic diversity (DI = 0.95), which suggests that there is epidemiological unrelated, with pulsetypes to particles serotypes. In addition, this study will help us create our database according to the PulseNet International Center for Disease Control and Prevention (CDC, USA).Keywords: infections, Electrophoresis, Epidemiology, Pulse-types. RESUMENLa creciente necesidad de vigilancia de las enfermedades infecciosas re-emergentes hace necesario la aplicación de métodos moleculares de caracterización como la Electroforesis en Gel de Campos Pulsados-PFGE. En la primera colecta, el análisis de macrorrestricción de S. aureus revela una frecuencia media (0.54) del pulsotipo II, en comparación a la segunda colecta donde se observa una mayor heterogeneidad genética. S. enterica presentó algunos pulsotipos idénticos y otros pulsotipos presentaron una diversidad genética (ID=0.95), lo que nos sugiere, que no existe una relación epidemiológica con pulsotipos o serotipos particulares. Además, este estudio nos ayudara a crear nuestra base de datos de acuerdo a la PulseNet International del Centro de Control y Prevención de Enfermedades (CDC, USA).

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Pulsed-field gel electrophoresis-based subtyping of DNA degradation-sensitive Salmonella enterica subsp. enterica serovar Livingstone and serovar Cerro isolates obtained from a chicken layer farm

Cited by 39 publications

References 15 publications

Methodologies for Salmonella enterica subsp. enterica Subtyping: Gold Standards and Alternatives

Methodologies for Salmonella enterica subsp. enterica Subtyping: Gold Standards and Alternatives

Current and Emerging Innovations for Detection of Food-Borne Salmonella

Caracterización molecular de bacterias patógenas mediante electroforesis en gel de campos pulsados (PFGE)

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