Pulsed electromagnetic fields rapidly modulate intracellular signaling events in osteoblastic cells: Comparison to parathyroid hormone and insulin

Schnoke, Matthew; Midura, Ronald J.

doi:10.1002/jor.20373

Cited by 52 publications

(35 citation statements)

References 39 publications

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“…Further, recent evidence shows that the signal transduction networks induced by PEMF include phosphorilation of the insulin receptor substrate-1 (IRS-1), which mediates IGF signaling [Schnoke and Midura, 2007]. In this light, a potential mechanism to explain our results concerning the analogous effect of PEMF and IGF-I on PG synthesis might include the IRS-1 phosphorylation.…”

Section: Discussionmentioning

confidence: 72%

Chondroprotective effects of pulsed electromagnetic fields on human cartilage explants

Ongaro

Pellati

Masieri

et al. 2011

Bioelectromagnetics

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This study investigated the effects of pulsed electromagnetic fields (PEMFs) on proteoglycan (PG) metabolism of human articular cartilage explants from patients with osteoarthritis (OA). Human cartilage explants, recovered from lateral and medial femoral condyles, were classified according to the International Cartilage Repair Society (ICRS) and graded based on Outerbridge scores. Explants cultured in the absence and presence of IL-1β were treated with PEMF (1.5 mT, 75 Hz) or IGF-I alone or in combination for 1 and 7 days. PG synthesis and release were determined. Results showed that explants derived from lateral and medial condyles scored OA grades I and III, respectively. In OA grade I explants, after 7 days exposure, PEMF and IGF-I significantly increased (35) S-sulfate incorporation 49% and 53%, respectively, compared to control, and counteracted the inhibitory effect of IL 1β (0.01 ng/ml). The combined exposure to PEMF and IGF-I was additive in all conditions. Similar results were obtained in OA grade III cartilage explants. In conclusion, PEMF and IGF-I augment cartilage explant anabolic activities, increase PG synthesis, and counteract the catabolic activity of IL-1β in OA grades I and III. We hypothesize that both IGF-I and PEMF have chondroprotective effects on human articular cartilage, particularly in early stages of OA.

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Section: Discussionmentioning

confidence: 72%

Chondroprotective effects of pulsed electromagnetic fields on human cartilage explants

Ongaro

Pellati

Masieri

et al. 2011

Bioelectromagnetics

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“…In animal models, PEMFs show enhanced early fracture healing, chondrogenesis, and reactive bone formation [Spadaro, 1997;Aaron et al, 2002;Midura et al, 2005;Fini et al, 2006]. In vitro studies with bone cells have generally supported the idea that PEMFs can modulate osteoblast proliferation, differentiation, and matrix formation [Lohmann et al, 2000;Aaron et al, 2004;Schnoke and Midura, 2007]. Mechanistic evidence for electromagnetic effects on specific osteoblast membrane signaling pathways has been accumulating, but there is no consensus yet on the primary biophysical events [Lohmann et al, 2000;Brighton et al, 2001;Aaron et al, 2002;Haddad et al, 2007;Kolomytkin et al, 2007;Schnoke and Midura, 2007;Fitzsimmons et al, 2008].…”

Section: Introductionmentioning

confidence: 93%

Electromagnetic effects on forearm disuse osteopenia: A randomized, double-blind, sham-controlled study

Spadaro

Short

Sheehe

et al. 2010

Bioelectromagnetics

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A randomized, double-blind, sham-controlled, feasibility and dosing study was undertaken to determine if a common pulsing electromagnetic field (PEMF) treatment could moderate the substantial osteopenia that occurs after forearm disuse. Ninety-nine subjects were randomized into four groups after a distal radius fracture, or carpal surgery requiring immobilization in a cast. Active or identical sham PEMF transducers were worn on the distal forearm for 1, 2, or 4 h/day for 8 weeks starting after cast removal ("baseline") when bone density continues to decline. Bone mineral density (BMD) and bone geometry were measured in the distal forearm by dual energy X-ray absorptiometry (DXA) and peripheral quantitative computed tomography (pQCT) at entry ("baseline") and 8, 16, and 24 weeks later. Significant average BMD losses after baseline were observed in the affected forearm at all time points (5-7% distally and 3-4% for the radius/ulna shaft). However, after adjusting for age, gender, and baseline BMD there was no evidence of a positive effect of active versus sham PEMF treatment on bone loss by DXA or pQCT for subjects completing all visits (n = 82, ∼20 per group) and for an intent-to-treat analysis (n = 99). Regardless of PEMF exposure, serum bone-specific alkaline phosphatase (BSAP) was normal at baseline and 8 weeks, while serum c-terminal collagen teleopeptide (CTX-1) was markedly elevated at baseline and less so at 8 weeks. Although there was substantial variability in disuse osteopenia, these results suggested that the particular PEMF waveform and durations applied did not affect the continuing substantial disuse bone loss in these subjects.

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“…PTH treatment resulted in CREB phosphorylation on the activating serine 133 residue [31]. The effects of PTH are primarily mediated by activating the cAMP/PKA signaling pathway through binding to G-protein-coupled receptors in target-cell membranes [32].…”

Section: Discussionmentioning

confidence: 99%

Endogenous Parathyroid Hormone Promotes Fracture Healing by Increasing Expression of BMPR2 through cAMP/PKA/CREB Pathway in Mice

Zhou

Jin

et al. 2017

Cell Physiol Biochem

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Background/Aims: Endogenous parathyroid hormone (PTH) plays an important role in fracture healing. This study investigated whether endogenous PTH regulates fracture healing by bone morphogenetic protein (BMP) and/or the transforming growth factor-β (TGF-β) signaling pathway. Methods: Eight-week-old wild-type (WT) and PTH-knockout (PTH KO) male mice were selected, and models of open right-femoral fracture were constructed. Fracture healing and callus characteristics of mice in the two groups were compared by X-ray, micro-computed tomography, histological, and immunohistochemical examinations. Bone marrow mesenchymal stem cells (BMMSCs) of 8-week-old WT and PTHKO male mice were obtained and induced into osteoblasts and chondrocytes. Results: We found that expression levels of Runt-related transcription factor (RUNX2), bone morphogenetic protein-receptor-type Ⅱ (BMPR2), phosphorylated Smad 1/5/8, and phosphorylated cyclic adenosine monophosphate-responsive element binding protein (CREB) in the callus of PTHKO mice were significantly decreased, whereas no significant difference in expression of SOX9, TGF-βR2,or pSMAD2/3 was observed between PTHKO and WT mice. Additionally, the activity of osteoblast alkaline phosphatase was low at 7 days post-induction, and was upregulated by addition of PTH or dibutyryl cyclic adenosine monophosphate (dbcAMP) to the cell culture. Furthermore, H89 (protein kinase A inhibitor)eliminated the simulating effects of PTH and dbcAMP, and a low concentration of cyclic adenosine monophosphate (cAMP) was observed in PTHKO mouse BMMSCs. Conclusion: These results suggested that endogenous PTH enhanced BMPR2 expression by a cAMP/PKA/CREB pathway in osteoblasts, and increased RUNX2 expression through transduction of the BMP/pSMAD1/5/8 signaling pathway.

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Pulsed electromagnetic fields rapidly modulate intracellular signaling events in osteoblastic cells: Comparison to parathyroid hormone and insulin

Cited by 52 publications

References 39 publications

Chondroprotective effects of pulsed electromagnetic fields on human cartilage explants

Chondroprotective effects of pulsed electromagnetic fields on human cartilage explants

Electromagnetic effects on forearm disuse osteopenia: A randomized, double-blind, sham-controlled study

Endogenous Parathyroid Hormone Promotes Fracture Healing by Increasing Expression of BMPR2 through cAMP/PKA/CREB Pathway in Mice

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