Pulsed electromagnetic field stimulates osteoprotegerin and reduces RANKL expression in ovariectomized rats

Zhou, Jun; Chen, Shiju; Guo, Hua; Xia, Lu; Liu, Huifang; Qin, Youwen; He, Chengqi

doi:10.1007/s00296-012-2499-9

Cited by 43 publications

(53 citation statements)

References 39 publications

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“…DEXA scanning also provides high precision and accuracy for evaluating the BMD of rats in vivo and in vitro [47]. Zhou et al reported that PEMF could increase serum E 2 levels and BMD in OVX rats [48]. The result in our study showed that BMD in the SHAM group was significantly increased above the BMD of the OVX group.…”

Section: Figuresupporting

confidence: 76%

Study on Pulsed Electromagnetic Fields Regulate the mRNA Expression of V-ATPase, CAII, NFAT2 and RANK in an Ovariectomized Rat Model of Osteoporosis Mechanism Analysis

Huang¹,

Chen²,

He³

et al. 2017

J Bioengineer & Biomedical Sci

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We investigated the mRNA expression of V-ATPase CA II, NFAT2, and RANK as well as bone mineral density (BMD) in bilaterally ovariectomized (OVX) rats treated with pulsed electromagnetic fields (PEMF), to explore the mechanism by which PEMF alter bone at the molecular level in vivo. 24 Sprague-Dawley female rats were randomly divided into three different groups: SHAM, OVX, ovariectomy with PEMF stimulation (PEMF+OVX). The PEMF frequency was 3.8 mT, 8 Hz, for 40 min per day. Eight rats in each group were treated for 28 days. At the end of the intervention, the body weight and BMD were measured. The mRNA expression of V-ATPase, CA II, NFAT2 and RANK were analyzed by real-time fluorescent-nested quantitative polymerase chain reaction (RT-PCR). The V-ATPase, CAII, NFAT2 and RANK genes show strong expression in the OVX group as compared with the SHAM group (P<0.001, respectively). The mRNAs expression of V-ATPase, CAII, NFAT2 and RANK in PEMF treatment group were significantly lower than that in OVX group, respectively (p=0.001, p=0.000, p=0.013, P=0.04, respectively). The V-ATPase, CAII and RANK mRNA expression in the PEMF+OVX group were higher than SHAM group (p=0.04, p=0.001, p=0.000, respectively). However, the NFAT2 mRNA expression did not show a statistically significant difference between the SHAM and the PEMF+OVX group (P=0.08). This study indicated that exposure to PEMF restrained bone resorption in order to elevate BMD, and this outcome occurred in part through RANK-signaling by the CN/NFAT pathway to regulate bone metabolism. Alternatively, PEMF may promote the BMD through regulating the expression of NFAT2 genes. It may have certain significance in the treatment target of osteoporosis.

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Section: Figuresupporting

confidence: 76%

Study on Pulsed Electromagnetic Fields Regulate the mRNA Expression of V-ATPase, CAII, NFAT2 and RANK in an Ovariectomized Rat Model of Osteoporosis Mechanism Analysis

Huang¹,

Chen²,

He³

et al. 2017

J Bioengineer & Biomedical Sci

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“…Many studies have shown the favorable effects of PEMFs on osteoporosis in OVX or hindlimb-suspended animals [6][7][8][9]. However, few studies have evaluated the effects of PEMFs on bone mass, bone microarchitecture, and bone strength together with their related signaling pathway mechanisms in animal models of diabetic osteopenia.…”

Section: Discussionmentioning

confidence: 99%

“…A previous study has reported that PEMFs can regulate OPG, RANKL, and macrophage colony-stimulating factor (M-CSF) concentrations in marrow culture system, thus controlling osteoclastogenesis and bone resorption [12]. We have also reported that PEMFs affect RANK and OPG expression in ovariectomized (OVX) rats [6]. Evidences have shown that Wnt/b-catenin signaling pathway plays an important role in bone metabolism.…”

Section: Introductionmentioning

confidence: 92%

“…The total RNA was reverse transcribed with a first-strand cDNA synthesis kit (Fermentas, USA), and real-time PCR was carried out in a FTC-2000 RealTime PCR machine (Funglyn, CA) according to our previous description [6]. Gene expression levels were normalized to the housekeeping gene b-actin.…”

Section: Real-time Polymerase Chain Reaction (Real-time Pcr)mentioning

confidence: 99%

“…As a safe and noninvasive physiotherapy, pulsed electromagnetic fields (PEMFs) have been suggested as a promising treatment for diabetic osteopenia. It has been reported that PEMFs can increase bone mineral density (BMD) in osteoporosis patients [5], prevent bone loss in ovariectomy-induced osteoporosis rats [6][7][8] and hindlimb-suspended rats [9], improve bone formation [10,11], and inhibit bone resorption [12] in vitro. It has reported that the cellular functions of bone marrow stromal cell [13], osteoblast [14,15], and osteoclast [16,17] are abnormal under diabetic conditions.…”

Section: Introductionmentioning

confidence: 99%

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Pulsed electromagnetic fields inhibit bone loss in streptozotocin-induced diabetic rats

Zhou

Liao

et al. 2014

Endocrine

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Evidences have shown that pulsed electromagnetic fields (PEMFs) can partially prevent bone loss in streptozotocin (STZ)-induced diabetic rats. However, the precise mechanisms accounting for these favorable effects are unclear. This study aimed to investigate the effects of PEMFs on bone mass and receptor activator of nuclear factor κB ligand (RANKL)/osteoprotegerin (OPG) and Wnt/β-catenin signaling pathway in STZ rats. Thirty 3-month-old Sprague Dawley rats were randomly divided into the following three groups (n = 10): control group (injection of saline vehicle), DM group (injection of STZ), and PEMFs group (injection of STZ + PEMFs exposure). One week following injection of STZ, rats in the PEMFs group were subject to PEMFs stimulus for 40 min/day, 5 days/week, and lasted for 12 weeks. After 12 week intervention, the results showed that PEMFs increased serum bone-specific alkaline phosphatase level and bone mineral density, and inhibited deterioration of bone microarchitecture and strength in STZ rats. Furthermore, PEMFs up-regulated the mRNA expressions of low-density lipoprotein receptor-related protein 5, β-catenin and runt-related gene 2 (Runx2), and down-regulated dickkopf1 in STZ rats. However, mRNA expressions of RANKL and OPG were not affected by PEMFs. PEMFs can prevent the diabetes-induced bone loss and reverse the deterioration of bone microarchitecture and strength by restoring Runx2 expression through regulation of Wnt/β-catenin signaling, regardless of its no glucose lowering effect.

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Effects of combined treatment with ibandronate and pulsed electromagnetic field on ovariectomy‐induced osteoporosis in rats

Zhou

Liao

Xie

et al. 2016

Bioelectromagnetics

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Ibandronate (IBN) and pulsed electromagnetic field (PEMF) have each shown positive effects for treating osteoporosis, but no study has evaluated the relative effects of these treatments combined. This study investigated the effects of IBN þ PEMF on bone turnover, mineral density, microarchitecture, and biomechanical properties in an ovariectomized (OVX) rat model of osteoporosis. Fifty 3-month-old rats were randomly apportioned to receive a sham-operation (n ¼ 10), or ovariectomy (n ¼ 40). The latter group was equally divided as the model (OVX control) or to receive IBN, PEMF, or IBN þ PEMF. Beginning the day after surgery, the IBN and IBN þ PEMF groups received weekly subcutaneous IBN; the PEMF and IBN þ PEMF groups were given daily PEMF during the same 12 weeks. After 12 weeks of treatments, biochemical parameters, bone mineral density (BMD), microarchitecture parameters, biomechanical properties, and some metabolic modulators that are involved in bone resorption were compared. The L5 lumbar vertebral body BMDs of the IBN, PEMF, and IBN þ PEMF groups were 121.6%, 119.5%, and 139.6%; maximum loads were 111.4%, 112.7%, and 121.9%; and energy to failure was 130.8%, 129.2%, and 154.9% of the OVX model, respectively. The IBN þ PEMF group had significantly lower levels of serum tartrate-resistant acid phosphatase 5b, and greater improvement in BMD, bone microarchitecture, and strength of the lumbar spine compared with monotherapy groups. Results showed that IBN þ PEMF had a more favorable effect on the lumbar spine in this osteoporosis model than did either monotherapy. Bioelectromagnetics. 38:31-40, 2017.

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Pulsed electromagnetic field stimulates osteoprotegerin and reduces RANKL expression in ovariectomized rats

Cited by 43 publications

References 39 publications

Study on Pulsed Electromagnetic Fields Regulate the mRNA Expression of V-ATPase, CAII, NFAT2 and RANK in an Ovariectomized Rat Model of Osteoporosis Mechanism Analysis

Study on Pulsed Electromagnetic Fields Regulate the mRNA Expression of V-ATPase, CAII, NFAT2 and RANK in an Ovariectomized Rat Model of Osteoporosis Mechanism Analysis

Pulsed electromagnetic fields inhibit bone loss in streptozotocin-induced diabetic rats

Effects of combined treatment with ibandronate and pulsed electromagnetic field on ovariectomy‐induced osteoporosis in rats

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