Pulsed electric field treatment for bacteria reduction and its impact on hospital wastewater

Gusbeth, Christian; Frey, Wolfgang; Volkmann, Holger; Schwartz, Thomas; Bluhm, H.

doi:10.1016/j.chemosphere.2008.11.066

Cited by 121 publications

(68 citation statements)

References 17 publications

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“…The phenomenon is considered to be a universal method and platform technology because all types of cells (animal, plant and microorganisms) are affected by electroporation (Miklavčič 2012). In fact, many electroporation-based applications have already been identified and developed, such as electrochemotherapy (ECT) of tumors , nonthermal irreversible ablation of tumors Maor et al 2009), gene therapy (Heller and Heller 2010), food preservation (Toepfl et al 2007) and others (Daugimont et al 2010;Gusbeth et al 2009;Ušaj et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Planning of Electroporation-Based Treatments Using Web-Based Treatment-Planning Software

et al. 2013

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Electroporation-based treatment combining high-voltage electric pulses and poorly permanent cytotoxic drugs, i.e., electrochemotherapy (ECT), is currently used for treating superficial tumor nodules by following standard operating procedures. Besides ECT, another electroporation-based treatment, nonthermal irreversible electroporation (N-TIRE), is also efficient at ablating deepseated tumors. To perform ECT or N-TIRE of deep-seated tumors, following standard operating procedures is not sufficient and patient-specific treatment planning is required for successful treatment. Treatment planning is required because of the use of individual long-needle electrodes and the diverse shape, size and location of deepseated tumors. Many institutions that already perform ECT of superficial metastases could benefit from treatmentplanning software that would enable the preparation of patient-specific treatment plans. To this end, we have developed a Web-based treatment-planning software for planning electroporation-based treatments that does not require prior engineering knowledge from the user (e.g., the clinician). The software includes algorithms for automatic tissue segmentation and, after segmentation, generation of a 3D model of the tissue. The procedure allows the user to define how the electrodes will be inserted. Finally, electric field distribution is computed, the position of electrodes and the voltage to be applied are optimized using the 3D model and a downloadable treatment plan is made available to the user.

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Section: Introductionmentioning

confidence: 99%

Planning of Electroporation-Based Treatments Using Web-Based Treatment-Planning Software

et al. 2013

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“…Nowadays, this effect is commonly known as electroporation [3] and is applied for gene and drug delivery to cells [4,5], for bacterial decontamination [6,7], food processing [8] and for cell ingredients extraction [9,10].…”

Section: Introductionmentioning

confidence: 99%

Effects of nanosecond pulsed electric field exposure on arabidopsis thaliana

Eing

Bonnet

Pacher

et al. 2009

IEEE Trans. Dielect. Electr. Insul.

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Seven days old seedlings of Arabidopsis thaliana, suspended in a 0.4 S/m buffer solution were exposed to nanosecond pulsed electric fields (nsPEF) with a duration of 10 ns, 25 ns and 100 ns. The electric field was varied from 5 kV/cm up to 50 kV/cm. The specific treatment energy ranged between 100 J/kg and 10 kJ/kg. Due to electroporation of the plasmamembrane of the plant cells, the seedlings completely died off, when 100 ns pulses and high electric field pulses were applied. But even at the highest specific treatment energies, 10 ns pulses had no lethal effect on the seedlings. An evaluation of the leaf area 5 and 7 days after pulsed electric field treatment revealed values twice the area of sham treated seedlings up to a specific treatment energy of 4 kJ/kg, when the applied field amplitude was low or the pulse duration 10 ns. A growth stimulating effect after short pulse exposition clearly could be detected. Contrary to the growth inhibiting effect of plasmamembrane electroporation on the seedlings, a growth stimulation by nsPEF treatment does not scale with the treatment energy within the applied parameter range.

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“…Nevertheless, intensive use of disinfectants may be associated with a change in pH value between different ranges. Therefore, different levels of pH change in hospital wastewater have been observed in different studies: 6.2 -7.1 [19], 5.1 -10.4 [20], and 7 -8 [21]. The present study on clinical laboratory wastewater also observed alkaline nature of the wastewater.…”

Section: Physiochemical Analysesmentioning

confidence: 65%

Contaminant Properties of Hospital Clinical Laboratory Wastewater: A Physiochemical and Microbiological Assessment

Akın¹

2016

JEP

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Hospital laboratory wastewater has been considered to significantly change the degree of contamination of especially the hospital wastewater. The present study investigated the hospital clinical laboratory wastewater and the pollution loads were assessed for pathogens, heavy metals, and organic materials. Composite samples were collected from clinical laboratory wastewater of a 350-bed hospital for a six-month period. Analyses for pH, TSS (Total Suspended Solid), BOD (Biochemical Oxygen Demand), COD (Chemical Oxygen Demand), PO4-P, and Cl as well as heavy metals (Cd, Pb, Zn, Cu, Cr, Co, Ni, Al, and Mn) were made in order to physiochemical properties of the samples. Bacterial isolation (Pseudomonas aeruginosa, Escherichia coli, Acinetobaumanii, CNS-CoagulaseNegative Staphylococcus) and antigen-antibody analyses were conducted in order to find the microbiological pollution load of the wastewater. As a result of the study it was found that the hospital clinical laboratory wastewater was alkaline and COD/BOD ratio reached to a range of 10 -12 in the wastewater. It was concluded that although the heavy metal concentrations were within the sewage discharge limits the said levels could pose health risk. It was also found that the wastewater entailed health risk due to pathogens.

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Pulsed electric field treatment for bacteria reduction and its impact on hospital wastewater

Cited by 121 publications

References 17 publications

Planning of Electroporation-Based Treatments Using Web-Based Treatment-Planning Software

Planning of Electroporation-Based Treatments Using Web-Based Treatment-Planning Software

Effects of nanosecond pulsed electric field exposure on arabidopsis thaliana

Contaminant Properties of Hospital Clinical Laboratory Wastewater: A Physiochemical and Microbiological Assessment

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