Pulsed electric field inactivation of diarrhoeagenic Bacillus cereus through irreversible electroporation

Rowan, Neil J.; MacGregor, S.J.; Anderson, John G.; Fouracre, R.A.; Farish, O.

doi:10.1046/j.1365-2672.2000.00772.x

Cited by 72 publications

(39 citation statements)

References 8 publications

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“…E LECTROPORATION, as a method for increasing cell membrane permeability to molecules that are otherwise poorly membrane permeant, is used in various biotechnological and biomedical applications, such as the introduction of molecules into cells [1], [2], cell fusion [3], [4], tissue ablation [5]- [7], and sterilization of water and liquid food [8]- [10]. In experimental settings, electroporation is normally performed by placing a biological sample (e.g., cell suspension or a small part of a tissue) between the electrodes and delivering a single electric pulse or a train of such pulses to the electrodes, thus creating the electric field between them.…”

Section: Introductionmentioning

confidence: 99%

Equivalent Pulse Parameters for Electroporation

Pucihar

Krmelj

Reberšek

et al. 2011

IEEE Trans. Biomed. Eng.

188

109

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Abstract-Electroporation-based applications require the use of specific pulse parameters for a successful outcome. When recommended values of pulse parameters cannot be set, similar outcomes can be obtained by using equivalent pulse parameters. We determined the relations between the amplitude and duration/number of pulses resulting in the same fraction of electroporated cells. Pulse duration was varied from 150 ns to 100 ms, and the number of pulses from 1 to 128. Fura 2-AM was used to determine electroporation of cells to Ca 2+ . With longer pulses or higher number of pulses, lower amplitudes are needed for the same fraction of electroporated cells. The expression derived from the model of electroporation could describe the measured data on the whole interval of pulse durations. In a narrower range (0.1-100 ms), less complex, logarithmic or power functions could be used instead. The relation between amplitude and number of pulses could best be described with a power function or an exponential function. We show that relatively simple two-parameter power or logarithmic functions are useful when equivalent pulse parameters for electroporation are sought. Such mathematical relations between pulse parameters can be important in planning of electroporationbased treatments, such as electrochemotherapy and nonthermal irreversible electroporation.

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Section: Introductionmentioning

confidence: 99%

Equivalent Pulse Parameters for Electroporation

Pucihar

Krmelj

Reberšek

et al. 2011

IEEE Trans. Biomed. Eng.

188

109

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show abstract

“…The advantages of such an electrotechnology include the potential retention of fresh-food characteristics and organoleptic qualities such as flavor, aroma, and texture (1). Previous research suggests that the application of PEF (with a magnitude usually greater than 20 kV cm Ϫ1 for short durations, such as 500 ns to 4 s) to liquids can inactivate susceptible microorganisms through irreversible electroporation of the cell membrane (7,10,12). The present studies were conducted to determine whether the application of PEF at different treatment temperatures (5, 20, and 50°C) effectively kills M. paratuberculosis cells in 0.1% (wt/vol) peptone water.…”

mentioning

confidence: 99%

Inactivation of Mycobacterium paratuberculosis by Pulsed Electric Fields

Rowan

MacGregor

Anderson

et al. 2001

Appl Environ Microbiol

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The influence of treatment temperature and pulsed electric fields (PEF) on the viability of Mycobacterium paratuberculosis cells suspended in 0.1% (wt/vol) peptone water and in sterilized cow's milk was assessed by direct viable counts and by transmission electron microscopy (TEM). PEF treatment at 50°C (2,500 pulses at 30 kV/cm) reduced the level of viable M. paratuberculosis cells by approximately 5.3 and 5.9 log 10 CFU/ml in 0.1% peptone water and in cow's milk, respectively, while PEF treatment of M. paratuberculosis at lower temperatures resulted in less lethality. Heating alone at 50°C for 25 min or at 72°C for 25 s (extended high-temperature, short-time pasteurization) resulted in reductions of M. paratuberculosis of approximately 0.01 and 2.4 log 10 CFU/ml, respectively. TEM studies revealed that exposure to PEF treatment resulted in substantial damage at the cellular level to M. paratuberculosis.

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“…Electroporation results in a significant increase in the membrane rupture and permeability which is termed as electro permeabilization. Electropermeabilization can be reversible or irreversible, depending on the degree of membrane organizational changes that results in cell death [13]. Literature explains that membrane permeability is increased in a considerable manner by increasing the strength of electric field.…”

Section: Microbial Inactivation By Pefmentioning

confidence: 99%

Pulsed Electric Field Technology in Food Preservation: A Review

Syed¹

2017

JNHFE

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Thermal inputs for food preservation generally dominate the food processing industry. However such treatments may lead to several undesirable changes in quality attributes and nutritional value of food. On the contrary, non-thermal preservation methods have a minimal impact on the sensory quality and nutritional status of food. Several non-thermal techniques have been involved in food preservation with an objective to maintain the nutritional properties and physic-chemical characteristics of food products. In this regard, pulsed electric field provides an alternative choice for various food products, particularly for liquid foods, for better preservation and maintenance of fresh-like quality aspects of food. Correspondingly, this memoir highlights the potential of pulsed electric field technology as an effective tool to preserve various foods with special reference to microbial inactivation. Additionally, principles, associated mechanistic approaches, effect of this treatment on food quality and problems linked with the consumer acceptance are also discussed in this script.

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Pulsed electric field inactivation of diarrhoeagenic Bacillus cereus through irreversible electroporation

Cited by 72 publications

References 8 publications

Equivalent Pulse Parameters for Electroporation

Equivalent Pulse Parameters for Electroporation

Inactivation of Mycobacterium paratuberculosis by Pulsed Electric Fields

Pulsed Electric Field Technology in Food Preservation: A Review

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